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In vivo Measurements of Sulcal Plaque pH After Topical Applications of Sorbitol and Sucrose in Rats Fed Sorbitol or Sucrose

Institute of Dental Research, University of Alabama School of Dentistry, and School of Public Health, University of Alabama at Birmingham, University Station, Birmingham, Alabama 35294

J.M. Navia

Institute of Dental Research, University of Alabama School of Dentistry, and School of Public Health, University of Alabama at Birmingham, University Station, Birmingham, Alabama 35294

To test whether adaptation to sorbitol could be observed in rat plaque, we made pH measurements of rat sulcal plaque in vivo, following topical application of 10% sorbitol solution. Rat pups were inoculated orally with S. mutans 6715 and fed diet MIT 305 (5% sucrose) for 16 days. Baseline sulcal plaque pH response of these rats to topical application of 10% sorbitol solution was measured. One group of 16 rats was then fed 20% sucrose in the diet, and a second group 20% sorbitol. After 13 days' feeding of the experimental diets (four days were used for accommodation to dose, and nine days at the 20% level for sorbitol), there was a significantly greater (p<0.01) drop in pH following topical application of 10% sorbitol in this group than in the sucrose-fed group. There was no difference in the pH response of the two groups to topical application of a 10% sucrose solution when tested six days later. The sulcal enamel caries score was significantly higher (p<0.001) in the sucrose group, but buccal enamel scores were similar in both groups.

Adaptation in rat plaque took place, and could be measured in vivo as an increased drop in sulcal plaque pH following topical application of sorbitol. It is not clear whether this adaptation was primarily due to selection of sorbitol-fermenting micro-organisms, or, more likely, by induction of sorbitol-specific enzymes. Relative to the sucrose-containing diet, the sorbitol diet was hypocariogenic, even under experimental conditions.

REFERENCES

• Birkhed, D.; Edwardsson, S.; Ahlden, M.-L.; and Frostell, G. (1979): Effects of 3 Months Frequent Consumption of Hydrogenated Starch Hydrolysate (Lycasin®), Maltitol, Sorbitol, and Xylitol on Human Dental Plaque, Acta Odontol Scand 37:103-115.
• Birkhed, D.; Edwardsson, S.; Svensson, B.; Mosko-Vitz, F.; and Frostell, G. (1978): Acid Production from Sorbitol in Human Dental Plaque, Arch Oral Biol 23:971-975.[Medline] [Order article via Infotrieve]
• Bohmer, U.; Kater, H.-J.; Schneider, P.-Th.; Welz-Bacher, H.; and Buttner, W. (1971): Comparative Cariogenicity of Sucrose Substitutes (Sorbitol, Lycasin, Calcium Sucrose Phosphate) in the Rat, J Dent Res 50:752, Abstr. No. 1.
• Brown, A.T. and Wittenberger, C.L. (1973): Mannitol and Sorbitol Catabolism in Streptococcus mutans, Arch Oral Biol 18:117-126.[CrossRef][Medline] [Order article via Infotrieve]
• Carlsson, J. (1968): A Numerical Taxonomic Study of Human Oral Streptococci, Odont Revy 19:137-160.
• Clark, R.; Hay, D.I.; Schram, C.J.; and Wagg, B.J. (1961): Removal of Carbohydrate Debris From the Teeth by Salivary Stimulation, Br Dent J 111:244-248.
• Cornick, D.E.R. and Bowen, W.H. (1972): The Effect of Sorbitol on the Microbiology of the Dental Plaque in Monkeys (Macaca irus), Arch Oral Biol 17:1637-1648.[CrossRef][Medline] [Order article via Infotrieve]
• Crowley, M.C.; Harner, V.; Bennett, A.S.; and Jay, P. (1956): Comparative Fermentability of Sorbitol, Glucose and Glycerol by Common Oral Microorganisms, J Am Dent Assoc 52:148-154.[Medline] [Order article via Infotrieve]
• Dallmeier, E.; Bestmann, H.-J.; and Kroncke, A. (1970): Uber den Abbau Von Glukose und Sorbit durch Plaque-Streptokokken, Dtsch Zahnärztl Z 25:887-898.[Medline] [Order article via Infotrieve]
• Edwardsson, S. (1968): Characteristics of Caries-inducing Human Streptococci Resembling Streptococcus mutans, Arch Oral Biol 13:637-646.[CrossRef][Medline] [Order article via Infotrieve]
• Finn, S.B. and Jamison, H.C. (1967): The Effect of a Dicalcium Phosphate Chewing Gum on Caries Incidence in Children: 30-Month Results, J Am Dent Assoc 74:987-995.[Medline] [Order article via Infotrieve]
• Firestone, A.R. and Navia, J.M. (1986): In vivo Measurements of Sulcal Plaque pH in Rats After Topical Applications of Xylitol, Sorbitol, Glucose, Sucrose, and Sucrose Plus 53 mM Sodium Fluoride, J Dent Res 65:44-48.[Abstract/Free Full Text]
• Fosdick, L.S.; Englander, H.R.; Hoerman, K.C.; and Kesel, R.G. (1957): A Comparison of pH Values of in vivo Dental Plaque After Sucrose and Sorbitol Mouth Rinses, J Am Dent Assoc 55:191-195.[Medline] [Order article via Infotrieve]
• Frostell, G. (1964): Quantitative Determination of the Acid Production from Different Carbohydrates in Suspensions of Dental Plaque Material, Acta Odontol Scand 22:457-475.[CrossRef][Medline] [Order article via Infotrieve]
• Frostell, G. (1965): Substitution of Fermentable Sugars in Sweets. In: Nutrition and Caries Prevention, G. Blix, Ed., Uppsala: Almquiat & Wiksells, pp. 60-66.
• Frostell, G.; Keyes, P.H.; and Larson, R.H. (1967): Effect of Various Sugars and Sugar Substitutes on Dental Caries in Hamsters and Rats, J Nutr 93:65-76.[Abstract/Free Full Text]
• Glass, R.L. (1983): A Two-Year Clinical Trial of Sorbitol Chewing Gum, Caries Res 17:365-368.[Medline] [Order article via Infotrieve]
• Graf, H. (1969): Telemetrie des pH der Interdentalplaque, Schweiz Mschr Zahnheilk 79:146-178.
• Grubb, T.C. (1945): Studies on the Fermentation of Sorbitol by Oral Microorganisms, J Dent Res 24:31-44.[Free Full Text]
• Guggenheim, B. (1968): Streptococci of Dental Plaques, Caries Res 2:147-163.[Medline] [Order article via Infotrieve]
• Gülzow, H.-J. (1971): Sorbitabbau im Speichel und in den Zahn. belagen des Menschen, Dtsch Zahnärztl Z 26:1121-1128.[Medline] [Order article via Infotrieve]
• Havenaar, R.; Drost, J.S.; de Stoppelaar, J.D.; Huis in't Veld, J.H.J.; and Backer Dirks, O. (1984): Potential Cariogenicity of Lycasin® 80/55 in Comparison to Starch, Sucrose, Xylitol, Sorbitol and L-Sorbose in Rats, Caries Res 18:375-384.[Medline] [Order article via Infotrieve]
• Havenaar, R.; Huis in't Veld, J.H.J.; Backer Dirks, O.; and de Stoppelaar, J.D. (1978): Some Bacteriological Aspects of Sugar Substitutes. In: Health and Sugar Substitutes, B. Guggenheim, Ed., Basel: S. Karger AG, pp. 192-198.
• Imfeld, T. (1977): Evaluation of the Cariogenicity of Confectionery by Intra-Oral Wire-Telemetry, Helv Odont Acta 21:1-28 (in) Schweiz Mschr Zahnheilk 87:437-464.
• Johansen, E. (1952): A New Technique for Oral Examination of Rodents, J Dent Res 31:361-365.[Free Full Text]
• Karle, E. and Buttner, W. (1971): Kariesbefall im Tierversuch nach Verabreichung von Sorbit, Xylit, Lycasin® und Calciumsaccharosephosphat, Dtsch Zahnärztl Z 26:1097-1108.
• Keyes, P.H. (1958): Dental Caries in the Molar Teeth of Rats. II. A Method for Diagnosing and Scoring Several Types of Lesions Simultaneously, J Dent Res 37:1088-1099.[Free Full Text]
• Leach, S.A. and Greene, R.M. (1981): Reversal of Fissure Caries in the Albino Rat by Sweetening Agents, Caries Res 15:508-511.[Medline] [Order article via Infotrieve]
• Miles, A.A. and Misra, S.S. (1938): The Estimation of the Bactericidal Power of the Blood, J Hygiene 38:732-748.
• Moller, I.J. and Poulsen, S. (1973): The Effect of Sorbitol-Containing Chewing Gum on the Incidence of Dental Caries, Plaque and Gingivitis in Danish School-Children, Community Dent Oral Epidemiol 1:58-67.[Medline] [Order article via Infotrieve]
• Mühlemann, H.R.; Regolati, B.; and Marthaler, T.M. (1970): The Effect on Rat Fissure Caries of Xylitol and Sorbitol, Helv Odont Acta 14:48-50.[Medline] [Order article via Infotrieve]
• Muhlemann, H.R.; Schmid, R.; Noguchi, T.; Imfeld, T.; and Hirsch, R.S. (1977): Some Dental Effects of Xylitol Under Laboratory and In Vivo Conditions, Caries Res 11:263-276.[Medline] [Order article via Infotrieve]
• Navia, J.M. (1977): Animal Models in Dental Research. University, Alabama The University of Alabama Press.
• Rosen, S. (1963): A Simple and Accurate Method for Determining Mean pH, Arch Oral Biol 8:779.[CrossRef]
• Shaw, J.H. and Griffiths, D. (1960): Partial Substitution of Hexitols for Sucrose and Dextrin in Caries-producing Diets, J Dent Res 39:377-384.[Free Full Text]
• Shockley, T.E.; Randles, C.I.; and Dodd, M.C. (1956): The Fermentation of Sorbitol by Certain Acidogenic Oral Microorganisms, J Dent Res 35:233-240.[Free Full Text]
• Slee, A.M. and Tanzer, J.M. (1983): The Repressible Metabolism of Sorbitol (D-Glucitol) by Intact Cells of the Oral Plaque-Forming Bacterium Streptococcus mutans, Arch Oral Biol 9:839-845.
• Westergren, G. and Krasse, B. (1978): Evaluation of a Micromethod for Determination of Streptococcus mutans and Lactobacillus Infection, J Clin Microbiol 7:82-83.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 65, No. 7, 1020-1023 (1986)
DOI: 10.1177/00220345860650070501


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