This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Pharoah, M.J. Articles by Heersche, J.N.M. Search for Related Content PubMed PubMed Citation Articles by Pharoah, M.J. Articles by Heersche, J.N.M. Social Bookmarking                         What's this?

Dexamethasone Inhibits Formation of Osteoclast-like Cells in Bone-marrow Cultures

Medical Research Council Group in Periodontal Physiology, 4384 Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada M5S 1A8

J.N.M. Heersche

Medical Research Council Group in Periodontal Physiology, 4384 Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada M5S 1A8

Our observations confirm the previously reported observation that multinucleated cells develop within a two-week period in cat bone-marrow cultures. Calcitonin induces cytoplasmic contraction in these multinucleated cells, which indicates that they resemble osteoclasts. After seven days of culture, cat bone-marrow cultures contain both non-attached mononuclear cells and attached mononuclear and polynuclear cells. We have cultured the attached and non-attached cell populations separately from day 7 onward and have observed the appearance of multinucleated cells in both culture systems. In both types of cultures, dexamethasone (10 ^-9-10^-7 mol/L) reduced the number of multinucleated cells. This effect of dexamethasone was more rapid in the cultures derived from non-attached cells (seven days) than in cultures derived from attached cells (14 days), suggesting that two different mechanisms are involved. Dexamethasone had no effect on the survival of multinucleated cells already formed.

REFERENCES

• Atkin, I.; Soskolne, W.A.; and Ornoy, A. (1981): Transplacental Effects of Cortisone Acetate on Calcification and Ossification of Long Bones in Mice, Metab Bone Dis Rel Res 3:199-207.
• Bressot, C.; Meunier, P.J.; Chapuy, M.C.: Lejeune, E.; Edouard, C.; and Darby, A. (1979): Histomorphometric Profile, Pathophysiology and Reversibility of Corticosteroid-induced Osteoporosis, Metab Bone Dis Rel Res 1:303-311.[CrossRef]
• Burger, E.H.; Van Der Meer, J.W.M.; and Nyweide, P. (1984): Osteoclast Formation from Mononuclear Phagocytes: Role of Bone Forming Cells, J Cell Biol 99:1901-1906.[Abstract/Free Full Text]
• Chambers, T.J. and Magnus, C.I. (1982): Calcitonin Alters Behaviour of Isolated Osteoclasts, J Pathol 136:27-39.[CrossRef][Medline] [Order article via Infotrieve]
• Chambers, T.J.; Revell, P.A.; Fuller, K.; and Athana-Sou, N.A. (1984): Resorption of Bone by Isolated Rabbit Osteoclasts, J Cell Sci 66:383-399.[Abstract]
• Evans, R.A.; Dunstan, C.R.; and Baylink, D.J. (1979): Histochemical-identification of Osteoclasts in Undecalcified Sections of Human Bone, Min Electrolyte Metab 2:179-185.
• Farrell, P.M.; Rikkers, H.; and Moel, D. (1976): Cortisol-Dihydrotachysterol Antagonism in a Patient with Hypothyroidism and Adrenal Insufficiency: Apparent Inhibition of Bone Resorption, J Clin Endo 42:953-957.
• Favini, P.; Ortolani, S.; Corghi, E.; Colombo, B.; Uigo, P.Z.; and Polli, E. (1982): Bone Histomorphometry in Corticosteroid-induced Osteopenia, Calcif Tissue Int 34: Absts. 32, 582.
• Hefley, T. and Stern, P.H. (1982): Isolation of Osteoclasts from Fetal Rat Long Bones, Calcif Tissue Int 34:480-487.[Medline] [Order article via Infotrieve]
• Hogg, N.; Shapiro, J.M.; Jones, S.J.; Slusarenko, M.; and Boyde, A. (1980): Lack of Fc Receptors on Osteoclasts, Cell Tissue Res 212:509-516.[Medline] [Order article via Infotrieve]
• Holtrop, M.E.; Cox, K.A.; and Glowacki, J. (1982): Cells of the Mononuclear Phagocytic System Resorb Implanted Bone Matrix: A Histologic and Ultrastructural Study, Calif Tissue Int 34:488-494.[CrossRef][Medline] [Order article via Infotrieve]
• Holtrop, M.E. and King, C.J. (1977): The Ultrastructure of the Osteoclast and Its Functional Implications, Clin Orthop Rel Res 123:177-196.
• Jaffe, H.L. (1975): Metabolic, Degenerative, and Inflammatory Diseases of Bones and Joints. Philadelphia: Lea & Febiger, pp. 633-639.
• Jee, W.S.S.; Park, H.Y.; Roberto, W.E.; and Kenner, G.H. (1970): Corticosteroid and Bone, Am J Anat 129:477-480.[CrossRef][Medline] [Order article via Infotrieve]
• Jowsey, J. and Riggs, B.Z. (1970): Bone Formation in Hypercorticism, Acta Endocrinol 63:21-30.[Abstract/Free Full Text]
• Krukowski, M. and Kahn, A.J. (1982): Induction Specificity of Mineralized Bone Matrix in Ectopic Osteoclast Differentiation, Calcif Tissue Int 34:474-479.[Medline] [Order article via Infotrieve]
• Liskova-Kiar, M. (1979): Mode of Action of Cortisol on Bone Resorption in Fetal Rat Fibula Cultured in vitro, Am J Anat 156:63-76.[CrossRef][Medline] [Order article via Infotrieve]
• Nelson, R.L. and Bauer, G.E. (1977): Isolation of Osteoclasts by Velocity Sedimentation at Unit Gravity, CalcifTissue Res 22:303-313.
• Osdoby, P.; Martini, M.C.; and Caplan, A.I. (1982): Isolated Osteoclasts and Their Presumed Progenitor Cells, J Exp Zool 224:331-344.[CrossRef][Medline] [Order article via Infotrieve]
• Owen, M. (1985): Lineage of Osteogenic Cells and Their Relationship to the Stromal System. In: Bone and Mineral Research, Vol. 13, W.A. Peck, Ed., Amsterdam: Elsevier, pp. 5-7.
• Raisz, L.G.; Trummel, C.L.; Wener, J.A.; and Simmons, H. (1972): Effect of Glucocorticoids on Bone Resorption in Tissue Culture, Endocrinology 90:961-967.[Abstract/Free Full Text]
• Suda, T.; Testa, N.G.; Allen, T.D.; Onions, D.; and Jarrett, O. (1983): Effect of Hydrocortisone on Osteoclasts Generated in Cat Bone Marrow Cultures, Calcif Tissue Int 35:82-86.[Medline] [Order article via Infotrieve]
• Testa, N.G.; Allen, T.D.; Laytha, L.G.; Onions, D.; and Jarrett, O. (1981): Generation of Osteoclasts in vitro, J Cell Sci 47:127-137.[Medline] [Order article via Infotrieve]
• Velasquez, M.J.; Haak-Frendscho, M.; and Osdoby, P. (1983): Calcitonin Receptors on Isolated Osteoclasts and Monocyte Derived Multinucleated Giant Cells. American Society for Bone and Mineral Research, 5th Annual Scientific Meeting, A20.
• Warshafsky, B.; Aubin, J.E.; and Heersche, J.N.M. (1985): Cytoskeleton Rearrangements During Calcitonin-induced Changes in Osteoclast Motility in vitro, Bone 6:179-185.

Journal of Dental Research, Vol. 65, No. 7, 1006-1009 (1986)
DOI: 10.1177/00220345860650070201


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. C. ADORNATO and K. A. PATICOFF Intralesional corticosteroid injection for treatment of central giant-cell granuloma J Am Dent Assoc, February 1, 2001; 132(2): 186 - 190. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Pharoah, M.J. Articles by Heersche, J.N.M. Search for Related Content PubMed PubMed Citation Articles by Pharoah, M.J. Articles by Heersche, J.N.M. Social Bookmarking                         What's this?