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A Theoretical Analysis of the Effects of Plaque Thickness and Initial Salivary Sucrose Concentration on Diffusion of Sucrose into Dental Plaque and its Conversion to Acid During Salivary Clearance
C. Dawes
Department of Oral Biology, Faculty of Dentistry, University of Manitoba, 780 Bannatyne Ave., Winnipeg, Canada R3E OW3
G.H. Dibdin
MRC Dental Unit, The Dental School, Lower Maudlin Street, Bristol, England BS1 2LY
A mathematical model, written in FORTRAN, has been developed to simulate the interrelated processes of salivary sucrose clearance from the mouth, diffusion of sucrose into dental plaque, and conversion of sucrose to acid and glucan. Reaction of acid with enamel is not included in the model. A total of 28 parameters can be varied by the user, and the relative importance of the different factors affecting acid formation can be assessed. The output of the program gives sucrose and acid concentrations and pH at different depths within the plaque. The initial variables studied were plaque thickness, the salivary sucrose concentration, and the duration of exposure of the plaque to sucrose.
Stephan curves typical of those recorded in vivo were generated by the model. With any particular salivary sucrose concentration, there was an optimum plaque thickness at which a minimum pH was achieved at the enamel surface, with very thin or thick plaque samples producing a smaller pH fall. With thick plaque, the minimum pH was often not achieved at the inner surface but at some intermediate depth, which may explain the location of early caries lesions in fissures. The extent of the pH fall at the inner surface and the duration of the pHminimum region of the Stephan curve were directly related to the initial salivary sucrose concentration and to the duration of exposure to sucrose prior to normal salivary clearance. Simulation of a water rinse at as short a time as two min after the beginning of normal salivary sugar clearance showed that this procedure had only a very small effect on the shape of the Stephan curve. Thus, the maximum salivary sucrose concentration achieved prior to normal salivary clearance appears to be a major factor which determines the extent of the pH fall at the enamel surface of the plaque.
REFERENCES
- Ashley, F.P. and Wilson, R.F. (1983): Comparability of the Biochemical Composition of Dental Plaque Samples Collected from 14- to 15-Year-Old Males Using Plastic and Metal Instruments, Caries Res 17:92-95. Birkhed, D. (1978): Automatic Titration Method for Determination of Acid Production from Sugars and Sugar Alcohols in Small Samples of Dental Plaque Material, Caries Res 12:128-136.[Medline]
[Order article via Infotrieve]
- Carlsson, J.; Newbrun, E.; and Krasse, B. (1969): Purification and Properties of Dextran-sucrase from Streplococcus sanguis, Arch Oral Biol 14:469-478.[CrossRef][Medline]
[Order article via Infotrieve]
- Crank, J. (1975): The Mathematics of Diffusion, 2nd ed., Oxford: Clarendon Press, Ch. 8.
- Dawes, C. (1983): A Mathematical Model of Salivary Clearance of Sugar from the Oral Cavity, Caries Res 17:321-334.[Medline]
[Order article via Infotrieve]
- Dawes, C. and Jenkins, G.N. (1962): Some Inorganic Constituents of Dental Plaque and their Relationship to Early Calculus Formation and Caries, Arch Oral Biol 7:161-172.[CrossRef][Medline]
[Order article via Infotrieve]
- Dibdin, G.H. (1981): Diffusion of Sugars and Carboxylic Acids through Human Dental Plaque in vitro. Arch Oral Biol 26:515-523.[CrossRef][Medline]
[Order article via Infotrieve]
- Dibdin, G.H. (1984): A Brief Survey of Recent in vitro Work on Diffusion of Small Ions and Molecules in Dental Plaque. In: Cariology Today, B. Guggenheim, Ed., Basel: S. Karger, pp. 191-198.
- Dibdin, G.H. and Reece, G.L. (1984): Computer Simulation of Diffusion with Reaction in Dental Plaque, Caries Res 18:181 (Abstr).
- Edgar, W.M. and Tatevossian, A. (1971): The Aqueous Phase of Plaque. In: Tooth Enamel II, R.W. Fearnhead and M.V. Stack, Eds., Bristol: John Wright and Sons Ltd., pp. 229-232.
- English, A.C. and Dole, M. (1950): Diffusion of Sucrose in Supersaturated Solutions, J Am Chem Soc 72:3261-3267.[CrossRef]
- Firestone, A.R. (1982): Effect of Increasing Contact Time of Sucrose Solution or Powdered Sucrose on Plaque pH in vivo, J Dent Res 61:1243-1244.[Abstract/Free Full Text]
- Frostell, G. (1964): Quantitative Determination of the Acid Production from Different Carbohydrates in Suspensions of Dental Plaque Material, Acta Odontol Scand 22:457-475.[CrossRef][Medline]
[Order article via Infotrieve]
- Frostell, G. (1969): Dental Plaque pH in Relation to Intake of Carbohydrate Products, Acta Odontol Scand 27:3-29.[CrossRef][Medline]
[Order article via Infotrieve]
- Geddes, D.A.M. (1975): Acids Produced by Human Dental Plaque Metabolism in situ, Caries Res 9:98-109.[Medline]
[Order article via Infotrieve]
- Goulet, D. and Brudevold, F. (1984): Salivary Glucose Clearance after
- Rinsing with Solutions of Different Concentrations of Glucose, Caries Res 18:481-487.
- Gray, J.A. (1966): Kinetics of Enamel Dissolution During Formation of Incipient Caries-like Lesions, Arch Oral Biol 11:397-421.[CrossRef][Medline]
[Order article via Infotrieve]
- Hamilton, I.R. and Ng, S.C.K. (1983): Stimulation of Glycolysis through Lactate Consumption in a Resting Cell Mixture of Streptococcus salivarius and Veillonella parvula, FEMS Microbiol Lett 20:61-65.[CrossRef]
- Hamilton, I.R.; Phipps, P.J.; and Ellwood, D.C. (1979): Effect of Growth Rate and Glucose Concentration on the Biochemical Properties of Streptococcus mutans Ingbritt in Continuous Culture, Infect Immun 26:861-869.[Abstract/Free Full Text]
- Harper, D.S. and Loesche, W.J. (1983): Effect of pH upon Sucrose and Glucose Catabolism by the Various Genogroups of Streptococcus mutans, J Dent Res 62:526-531.[Abstract/Free Full Text]
- Hayes, M.L. and Roberts, K.R. (1978): The Breakdown of Glucose, Xylitol and Other Sugar Alcohols by Human Dental Plaque Bacteria, Arch Oral Biol 23:445-451.[CrossRef][Medline]
[Order article via Infotrieve]
- Higuchi, W.I.; Young, F.; Lastra, J.L.; and Koulourides, T. (1970): Physical Model for Plaque Action in the Tooth-Plaque-Saliva System, J Dent Res 49:47-60.[Abstract/Free Full Text]
- Imfeld, T.N. (1983): Identification of Low Caries Risk Dietary Components, Monographs in Oral Science, Vol. 11, H.M. Myers, Ed., Basel: Karger, p. 67.
- James, M.L.; Smith, G.M., and Wolford, J.C. (1977): Applied Numerical Methods for Digital Computation with FORTRAN and CSMP, 2nd ed., New York, NY: IEP - A Dun-Donnelly Publisher, pp. 540-548. Kleinberg, I. (1961): Studies on Dental Plaque. I. The Effect of Different Concentrations of Glucose on the pH of Dental Plaque in vivo, J Dent Res 40:1087-1111.[Free Full Text]
- Kleinberg, I.; Kanapka, J.A.; Chatterjee, R.; Craw, D.; D'An-Gelo, N.; and Sandham, H.J. (1979): Metabolism of Nitrogen by the Oral Mixed Bacteria. In: Saliva and Dental Caries, I. Kleinberg, S.A. Ellison, and I.D. Mandel, Eds., Sp. Suppl., Microbiology Abstracts, New York: Information Retrieval, Inc., pp. 357-377.
- Lagerlof, F. and Dawes, C. (1984): The Volume of Saliva in the Mouth Before and After Swallowing, J Dent Res 63:618-621.[Abstract/Free Full Text]
- Lagerlof, F. and Dawes, C. (1985): Effect of Sucrose as a Gustatory Stimulus on the Flow Rates of Parotid and Whole Saliva, Caries Res 19:206-211.[Medline]
[Order article via Infotrieve]
- Lagerlof, F.; Dawes, R.; and Dawes, C. (1985): The Effects of Different Concentrations of Sucrose, Fructose, and Glucose on pH Changes by Streptococcus mitior in an Artificial Mouth, J Dent Res 64:405-410. Minah, G.E. and Loesche, W.J. (1977): Sucrose Metabolism in Resting-cell Suspensions of Caries-Associated and Non-Caries-Associated Dental Plaque, Infect Immun 17:43-54.[Abstract/Free Full Text]
- Newbrun, E. (1977): Polysaccharide Synthesis in Plaque. In: Proceedings, Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Sp. Supp., Microbiology Abstracts, Vol. III, pp. 649-664. Roberts, K.R. and Hayes, M.L. (1980): Effects of 2-deoxy D-glucose and other Sugar Analogues on Acid Production from Sugars by Human Dental Plaque Bacteria, Scand J Dent Res 88:201-209.[Medline]
[Order article via Infotrieve]
- Schachtele, C.F. and Harlander, S.K. (1984): Will the Diets of the Future be Less Cariogenic? J Can Dent Assoc 50:213-219. Silverstone, L.M.; Johnson, N.W.; Hardie, J.M.; and Williams, R.A.D. (1981): Dental Caries Aetiology, Pathology and Prevention, London: Macmillan Press, p. 135.
- Sreebny, L.M.; Chatterjee, R.; and Kleinberg, I. (1985): Clearance of Glucose and Sucrose from the Saliva of Human Subjects, Arch Oral Biol 30:269-274.[CrossRef][Medline]
[Order article via Infotrieve]
- Stephan, R.M. and Miller, B.F. (1943): A Quantitative Method for Evaluating Physical and Chemical Agents which Modify Production of Acid in Bacterial Plaques on Human Teeth, J Dent Res 22:45-51.[Free Full Text]
- Strålfors, A. (1950): The Bacterial Chemistry of Dental Plaques, Odont Tidsk 58:155-341.
- Tatevossian, A. (1981): The Chemistry of Plaque Fluid. In: Frontiers of Oral Physiology, Vol. 3, The Environment of the Teeth, D.B. Ferguson and Y. Kawamura, Eds., Basel: Karger, pp. 66-77.
- Zengo, A.N. and Mandel, I.D. (1972): Sucrose Tasting and Dental Caries in Man, Arch Oral Biol 17:605-607.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 65, No. 2,
89-94 (1986)
DOI: 10.1177/00220345860650021701
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