Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Leggott, P.J.
Right arrow Articles by Jacob, R.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leggott, P.J.
Right arrow Articles by Jacob, R.A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Response of Lingual Ascorbic Acid Test and Salivary Ascorbate Levels to Changes in Ascorbic Acid Intake

P.J. Leggott

School of Dentistry, University of California-San Francisco, San Francisco, California 94143

P.B. Robertson

School of Dentistry, University of California-San Francisco, San Francisco, California 94143

D.L. Rothman

School of Dentistry, University of California-San Francisco, San Francisco, California 94143

P.A. Murray

School of Dentistry, University of California-San Francisco, San Francisco, California 94143

R.A. Jacob

USDA-ARS, Western Human Nutrition Research Center, San Francisco, California 94129

This study sought to determine whether the lingual ascorbic acid test (LAAT) and measurement of salivary ascorbate refXect plasma and leukocyte ascorbate levels during controlled periods of ascorbic acid depletion and supplementation.

Eleven healthy non-smoking men, aged 19-28 years, ate a diet that was repeated every seven days and was adequate in all nutrients except ascorbic acid (AA). This basal diet, which provided less than 5 mg of AA per day, was supplemented with 60 mg of AA per day for two weeks, 0 mg (placebo) per day for four weeks, 600 mg per day for three weeks, and 0 mg per day for four weeks. Oral examinations, the lingual ascorbic acid test, and measurement of salivary, plasma, and leukocyte ascorbate concentrations were conducted throughout the study.

Ascorbic acid concentrations in plasma and leukocytes responded rapidly to changes in vitamin C intake. LAAT-derived ascorbate values were unrelated to ascorbic acid intake and plasma and leukocyte ascorbate concentrations. Salivary ascorbate levels approached the lower limits of detection of the assay and remained constant throughout the investigation. Oral hygiene was consistently excellent, and no severe mucosal or periodontal changes were observed. It was concluded that lingual ascorbic acid test values and salivary ascorbate levels are not related to changes in ascorbic acid intake and are not consistent with plasma or leukocyte ascorbate concentrations.

REFERENCES

  • Bates, J.F.; Hughes, R.E.; and Hurley, R.J. (1972): Ascorbic Acid Status in Man: Measurement of Salivary, Plasma and White Blood Cell Concentration, Arch Oral Biol 17:1017-1020.[CrossRef][Medline] [Order article via Infotrieve]
  • Cheraskin, E. and Ringsdorf, W.M., Jr. (1968a): A Lingual Vitamin C Test I. Reproducibility, Int Z Vitam Erndhrungsforsch 38:114-117.
  • Cheraskin, E. and Ringsdorf, W.M., Jr. (1968b): A Lingual Vitamin C Test: III. Relationship to Plasma Ascorbic Acid Level, Int Z Vitam Ernährungsforsch 38:120-122.
  • Cheraskin, E. and Ringsdorf, W.M., Jr. (1968c): A Lingual Vitamin C Test: V. A Study in Dietary Relationships, Int Z Vitam Ernährungsforsch 38:254-256.
  • Cheraskin, E. and Ringsdorf, W.M., Jr. (1970): A Lingual Vitamin C Test: XX. Effect of Multivitamin-Mineral Supplementation upon Vitamin C State, J Oral Med 25:118-120.[Medline] [Order article via Infotrieve]
  • Cheraskin, E.; Ringsdorf, W.M., Jr.; and El-Ashiry, G. (1964): A. Lingual Vitamin C Test, Int Z Vitam Erndhrungsforsch 34:31-38.
  • Clark, J.W.; Cheraskin, E.; and Ringsdorf, W.M., Jr. (1970): Diet and the Periodontal Patient, Springfield, IL: C.C. Thomas.
  • Daniels, T.; Powell, M.; Sylvester, R.; and Talal, N. (1979): An Evaluation of Salivary Scintigraphy in Sjogren's Syndrome, Arthritis Rheum 22:809-814.[Medline] [Order article via Infotrieve]
  • Dreizen, S.; Gilley, E.J.; Mosney, J.J.; and Spies, T.D. (1955): The Distribution of Selected Vitamins in Human Whole Saliva, Int Z Vitam Ernährungsforsch 26:257-262.
  • Feller, R.P.; Black, H.S.; and Shannon, I.L. (1975): Evidence for Absence of Ascorbic Acid in Human Saliva, Arch Oral Biol 20:563-566.[CrossRef][Medline] [Order article via Infotrieve]
  • Freeman, J.T. and Hafkesbring, R. (1951): Comparative Study of Ascorbic Acid Levels in Gastric Secretion, Blood, Urine and Saliva, Gastroenterol 18:225-229.
  • Giza, T. and Weclawowicz, J. (1960): Perlingual Method for Evaluating the Vitamin C Content of the Body: A Rapid Diagnostic Test for Vitamin C Undernutrition, Int Z Vitam Erndhrungsforsch 30:327-332.
  • Glavind, J.; Granados, H.; Hansen, L.A.; Schilling, K.; Kruse, I.; and Dali, H. (1948): The Presence of Vitamins in the Saliva, Int Z Vitam Ernährungsforsch 20:234-238.
  • Graves, C.E. and Fotos, P.G. (1982): Ascorbic Acid Nutritional Screening, Dent Hygiene, October:29-47.
  • Hauffman, H.T.; Levering, R.P.; and De Vriss, K. (1983): A Single Centrifugation Step Method for the Isolation of Erythrocytes, Granulocytes and Mononuclear Cells on Continuous Density Gradients of Percoll, J Immunol Meth 57:1-7.[CrossRef][Medline] [Order article via Infotrieve]
  • Hess, W.C. and Smith, B.T. (1949): The Ascorbic Acid Content of the Saliva of Carious and Noncarious Individuals, J Dent Res 28:507-511.[Free Full Text]
  • Kien, L.J. and McDermott, F.T. (1972): Screening Ascorbic Acid Deficiency by a Lingual Test, Med J Aust 2:420-423.[Medline] [Order article via Infotrieve]
  • King, D.R. and Little, J.W. (1970): Lingual Ascorbic Acid Test, J Oral Med 25:107-111.[Medline] [Order article via Infotrieve]
  • Kutnink, M.A.; Skala, J.H.; Sauberlich, H.E.; and Omaye, S.T. (1985): Simultaneous Determination of Ascorbic Acid, Isoascorbic Acid (Erythorbic Acid) and Uric Acid in Human Plasma by High Performance Liquid Chromatography with Amperometric Detection, J Liq Chromatog 8:31-46.[CrossRef]
  • Little, J.W. and Owsley, C.L. (1965): The Lingual Ascorbic Acid Test, Odontol Rev 16:91-96.
  • Loë, H. and Silness, J. (1963): Periodontal Disease in Pregnancy. I. Prevalence and Severity, Acta Odontol Scand 21:533-551.[Medline] [Order article via Infotrieve]
  • Makila, E. and Kirveskari, P. (1969): A Study of Ascorbic Acid in Human Saliva, Arch Oral Biol 14:1285-1292.[CrossRef][Medline] [Order article via Infotrieve]
  • Omaye, S.T.; Tillotson, J.A.; and Sauberlich, H.E. (1982): Metabolism of L-Ascorbic Acid in the Monkey. In: Ascorbic Acid: Chemistry, Metabolism and Uses, P.A. Seib and B.M. Tolbert, Eds., Washington, DC: American Chemical Society, pp. 317-334.
  • Omaye, S.T.; Turnbull, J.D.; and Sauberlich, H.E. (1979): Selected Methods for the Determination of Ascorbic Acid in Animal Cells, Tissues, and Fluids. Methods Enzymol 62:3-11.[Medline] [Order article via Infotrieve]
  • Randolph, P.; Wilson, T.J.; Roth, G.D.; and Young, R. (1974): Evaluation of Lingual Ascorbic Acid Test, J Oral Med 29:8-12.[Medline] [Order article via Infotrieve]
  • Ringsdorf, W.M., Jr. and Cheraskin, E. (1978): The Lingual Ascorbic Acid Test, Quint Int 12:81-85.
  • Sauberlich, H.E.; Dowdy, R.P.; and Skala, J.H. (1974): Laboratory Tests for Assessment of Nutritional Status, Cleveland: CRC Critical Reviews in Clinical Laboratory Sciences.
  • Segal, A.W.; Fortunato, A.; and Herd, T. (1980): A Rapid Single Centrifugation Step Method for the Separation of Erythrocytes, Granulocytes and Mononuclear Cells on Continuous Density Gradients of Percoll, J Immunol Methods 32:203-214.
  • Silness, J. and Loë, H. (1964): Periodontal Disease in Pregnancy. II. Correlation between Oral Hygiene and Periodontal Condition. Acta Odontol Scand 22:121-135.[Medline] [Order article via Infotrieve]
  • Sproles, A. and Shaeffer, L. (1974): An Advanced Design of the Carlson-Crittenden Cup for Collection of Parotid Fluid, Bio-mater Med Devices Artif Organs 2:95-101.[Medline] [Order article via Infotrieve]
  • Tillotson, J.A. and O'Connor, R.J. (1980): Ascorbic Requirements in the Trained Monkey as Determined by Blood Ascorbic Levels. Int J Vitam Nutr Res 50:171-180.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 65, No. 2, 131-134 (1986)
DOI: 10.1177/00220345860650020801
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
P.J. Leggott, P.B. Robertson, R.A. Jacob, J.J. Zambon, M. Walsh, and G.C. Armitage
Effects of Ascorbic Acid Depletion and Supplementation on Periodontal Health and Subgingival Microflora in Humans
Journal of Dental Research, December 1, 1991; 70(12): 1531 - 1536.
[Abstract] [PDF]

Home page
 Arch DermatolHome page
E. F. Sherertz
Why Sadie Licks Her Wounds
Arch Dermatol, October 1, 1988; 124(10): 1499 - 1500.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Leggott, P.J.
Right arrow Articles by Jacob, R.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leggott, P.J.
Right arrow Articles by Jacob, R.A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?