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Journal of Dental Research
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Effects of Extracellular Plaque Components on the Chlorhexidine Sensitivity of Strains of Streptococcus mutans and Human Dental Plaque

L, E. Wolinsky

Section of Oral Biology, University of California School of Dentistry, Los Angeles, California 90024, USA

W.R. Hume

Department of Dentistry, The University of Adelaide, Adelaide 5000, Australia

An in vitro study was undertaken to determine the effects of sucrose-derived extracellular plaque components on the sensitivity of selected oral bacteria to chlorhexidine (CX). Cultures of Streptococcus mutans HS-6, OMZ-176, Ingbritt C, 6715-wtl3, and pooled human plaque were grown in trypticase soy media with or without 1 % sucrose. The sensitivity to CX of bacteria grown in each medium was determined by fixed-time exposure to CX and subsequent measurement of 3H-thymidine uptake. One-hour exposure to CX at concentrations of 10-4 M (0.01% w/v) or greater substantially inhibited subsequent cellular division among all the S. mutans strains and human plaque samples tested. An IC50 (the CX concentration which depressed 3H-thymidine incorporation to 50% of control level) of close to 10-4 M was noted for S. mutans strains HS- 6, OMZ-176, and 6715-wt13 when grown in the presence of sucrose. The same strains grown in cultures without added sucrose showed about a ten-fold greater sensitivity to CX (IC50 close to 10 -5 M). A three-fold difference was noted for S. mutans Ingbritt C. Only a slight increase in the IC50 was noted for the plaque samples cultured in sucrose-containing media, but their threshold for depression of 3H-thymidine uptake by CX was lower than that for the sucrose-free plaque samples. The study showed that extracellular products confer some protection against CX to the bacteria examined, and provided an explanation for the disparity between clinically-recommended concentrations for plaque suppression and data on in vitro susceptibility. Also, when compared with similarly-derived indices of susceptibility of mammalian cells to CX, the data obtained give rise to new possibilities for therapeutic use of CX or other bisguanides in dentistry.

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Journal of Dental Research, Vol. 64, No. 8, 1051-1054 (1985)
DOI: 10.1177/00220345850640080501
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This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
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Citing Articles
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Right arrow Articles by Wolinsky, L, E.
Right arrow Articles by Hume, W.R.
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PubMed
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Right arrow Articles by Wolinsky, L, E.
Right arrow Articles by Hume, W.R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?