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Antigenic Variation of Indigenous Streptococci

Department of Basic Dental Sciences, College of Dentistry, Department of Microbiology and Cell Science, Institute of Food and Agricultural Science, Periodontal Disease Research Center, University of Florida, Gainesville, Florida 32610

W.B. Clark

Department of Basic Dental Sciences, College of Dentistry, Department of Microbiology and Cell Science, Institute of Food and Agricultural Science, Periodontal Disease Research Center, University of Florida, Gainesville, Florida 32610

A.S. Bleiweis

Department of Microbiology and Cell Science, Institute of Food and Agricultural Science, Periodontal Disease Research Center, University of Florida, Gainesville, Florida 32610

Isolates of Group D streptococci indigenous to the murine oral cavity were studied to detect the occurrence of antigenic variation. Group D streptococci cultured from molar homogenates of Balb/c mice were randomly selected for study on the basis of distinctive colony morphology. Isolates obtained over a 12-week period were biotyped using the API 20S system, and subjected to Lancefield extraction and rocket immunoelectrophoresis for serotyping. All isolates were compared with an arbitrarily selected standard test strain (W1S-1) isolated the first week of the first experimental series. Four biotypes were encountered during the first week of two experimental series. Two very unusual biotypes detected during the first experimental series persisted throughout that series, as did two more common biotypes throughout the second experimental series. Anti-W1S-1 serum produced three precipitin bands (antigens O, D, and K) against WIS-1 Lancefield extract and against the respective biotypes detected during the first week of the two series. Of the three antigens detected, only the group antigen (D) did not vary during either experimental series. Antigenic variants lacking the O or K antigen and bearing these distinctive phenotypes were repeatedly isolated in subsequent weeks. Ultimately, 16% of 190 strains isolated during the first series and 26% of 167 strains isolated during the second series proved to be antigenic variants of the predominant biotypes detected in both series.

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Journal of Dental Research, Vol. 64, No. 8, 1039-1045 (1985)
DOI: 10.1177/00220345850640080301


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Beem, J.E. Articles by Bleiweis, A.S. Search for Related Content PubMed PubMed Citation Articles by Beem, J.E. Articles by Bleiweis, A.S. Social Bookmarking                         What's this?