Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Bertolami, C.N.
Right arrow Articles by Ellis, D.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertolami, C.N.
Right arrow Articles by Ellis, D.G.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Identification and Characterization of Rabbit Buccal Mucosal Hyaluronidase

C.N. Bertolami

Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Shriners Burns Institute, Boston, Massachusetts 02114

D.G. Ellis

Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Shriners Burns Institute, Boston, Massachusetts 02114

This study establishes the existence of a mammalian buccal mucosal hyaluronidase having properties typical of a lysosomal enzyme. Mucosal hyaluronidase exhibited a distinct substrate preference for hyaluronic acid, generated saturated de-polymerization products by endoglycosidic hydrolysis, and possessed a pH optimum of 3.5 and an isoelectric point of 5.9.

REFERENCES

  • Alexander, S.A.; Swerdloff, M.; Ceen, R.; and Bertolami, C.N. (1980): Hyaluronidase Activity in Human Premolar and Third Molar Dental Sacs, Arch Oral Biol 25:207-209. Attstrom, R. (1970): Presence of Leukocytes in Crevices of Healthy and Inflamed Gingiva, J Periodont Res 5:42-47. Bertolami, C.N. and Donoff, R.B. (1982): Identification, Characterization, and Partial Purification of Skin Wound Hyaluronidase, J Invest Derm 79:417-421.[CrossRef][Medline] [Order article via Infotrieve]
  • Bitter, T. and Muir, H.M. (1962): A Modified Uronic Acid Carbazole Reaction, Anal Biochem 4:330-334.[CrossRef][Medline] [Order article via Infotrieve]
  • Bollet, A.J.; Bonner, W.M.; and Nance, J.L. (1963): The Presence of Hyaluronidase in Various Mammalian Tissues, J Biol Chem 238:3522-3527.[Free Full Text]
  • Dallaird, F.J.; Ciancio, S.G.; and Hausmann, E. (1969): Hyaluronidase Activity of the Gingival Environment, J Periodont 40:17-19.[Medline] [Order article via Infotrieve]
  • Donoff, R.B.; Swann, D.A.; and Schweidt, S.H. (1984): Glycosaminoglycans of Normal and Hypertrophic Human Scar, Exp Molec Pathol 40:13-20.[CrossRef][Medline] [Order article via Infotrieve]
  • Fairbanks, G.; Steck, T.L.; and Wallach, D.F.H. (1971): Electrophoretic Analysis of the Major Polypeptides of the Human Erythrocyte Membrane, Biochem 10:2606-2617. Fenger, M. (1982): Purification of Human Serum Hyaluronidase Using Chromatofocusing, J Chromatog 240:173-179. Goggins, J.F.; Fullmer, H.M.; and Steffek, A.J. (1968): Hyaluronidase Activity of Human Gingiva, Arch Pathol 85:272-274.[Medline] [Order article via Infotrieve]
  • Gold, E.W. (1982): Purification and Properties of Hyaluronidase from Human Liver, Biochem J 205:69-74.[Medline] [Order article via Infotrieve]
  • Gupta, G.S. and Goldberg, E. (1981): Isolation, Properties, Immunological Specificity and Localization of Mouse Testicular Hyaluronidase, Biochem Biophys Acta 657:364-373.[Medline] [Order article via Infotrieve]
  • Hatae, Y. and Makita, A. (1975): Colorimetric Determination of Hyaluronate Degraded by Streptomyces hyaluronidase, Anal Biochem 64:30-36.[CrossRef][Medline] [Order article via Infotrieve]
  • Hopps, R.M. and Prout, R.E.S. (1972): Identification of Tissue and Bacterial Hyaluronidase in Human Saliva, J Periodont Res 7:236-241.[CrossRef][Medline] [Order article via Infotrieve]
  • Ivaska, K. (1981): Effect of Extracellular Glycosaminoglycans on the Synthesis of Collagen and Proteoglycans by Granulation Tissue Cells, Acta Physiol Scand (Suppl) 494:1-53. Klinkhamer, J.M. (1968): Quantitative Evaluation of Gingivitis and Periodontal Disease. I - The Orogranulocytic Migratory Rate, Periodontics 6:207-211.[Medline] [Order article via Infotrieve]
  • Lewy, G.A. and Marsh, C.A. (1959): Preparation and Properties of BETA-Glucuronidase, Adv Carbohyd Chem 14:381-428. Lowry, 0.; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
  • Mahler, I.R. and Lisanti, V.F. (1952): Hyaluronidase-Producing Microorganisms from Human Saliva, Oral Surg 5:1235-1240. Nord, C.E. (1970): Studies on Oral Hyaluronidases, Acta Odontol Scand 28 (Suppl. 56):1-12.[Medline] [Order article via Infotrieve]
  • Orkin, R.W.; Jackson, G.; and Toole, B.P. (1977): Hyaluronidase Activity in Cultured Chick Embryo Skin Fibroblasts, Biochem Biophys Res Commun 77:132-138.[CrossRef][Medline] [Order article via Infotrieve]
  • Orkin, R.W. and Toole, B.P. (1978): Hyaluronidase Activity and Hyaluronate Content of the Developing Chick Embryo Heart, Dev Biol 66:308-320.[CrossRef][Medline] [Order article via Infotrieve]
  • Orkin, R.W. and Toole, B.P. (1980): Chick Embryo Fibroblasts Produce Two Forms of Hyaluronidase, J Cell Biol 85:248-257. Partridge, S.M. (1948): Filter-paper Partition Chromatography of Sugars, Biochem 42:238-253.[CrossRef]
  • Polansky, J.R.; Toole, B.P.; and Gross, J. (1974): Brain Hyaluronidase: Changes in Activity During Chick Development, Science 183:862-864.[Abstract/Free Full Text]
  • Polansky, J.R. and Toole, B.P. (1976): Hyaluronidase Activity During Thyoxine-induced Tadpole Metamorphosis, Dev Biol 53:30-35.[CrossRef][Medline] [Order article via Infotrieve]
  • Rautela, G.S. and Abramson, C. (1973): Crystallization and Partial Characterization of Staphylococcus aureus Hyaluronate Lyase, Arch Biochem Biophys 158:687-694.[CrossRef][Medline] [Order article via Infotrieve]
  • Reissig, J.L.; Strominger, J.L.; and Leloir, L.F. (1955): A Modified Colorimetric Method for the Estimation of N-acetylamino Sugars, J Biol Chem 217:959-966.[Free Full Text]
  • Righetti, D.G. and Drysdale, J.W. (1976): Isoelectric Focusing. In: Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 5, S. Work and E. Work, Eds., Amsterdam: North Holland, pp. 337-590.
  • Rovelstad, G.H.; Geller, J.H.; and Cohen, A.H. (1958): The Hyaluronidase Activity of Saliva. II. The Relationship of Hyaluronidase Activity to Dental Caries Experience, Gingivitis and Oral Hygiene in Young Male Adults, J Dent Res 57:104-118. Sahu, S.C. (1981): Hyaluronidase Activity in Alveolar Secretions of Human Lung, Biochem Med 25:56-61.[CrossRef][Medline] [Order article via Infotrieve]
  • Sakamoto, N.;Okamoto, H.; and Okuda, K. (1978): Qualitative and Quantitative Analyses of Bovine Gingiva Glycosaminoglycans, Arch Oral Biol 23:983-987.[CrossRef][Medline] [Order article via Infotrieve]
  • Sakamoto, N.; Okamoto, H.;and Okuda, K. (1979): Qualitative and Quantitative Analyses of Bovine, Rabbit, and Human Dental Pulp Glycosaminoglycans, JDent Res 58:646-655. Sakamoto, N.; Nakajima, T.; Ikunaga, K.; Shidahara, H.; Okamoto, H.; and Okuda, K. (1981): Identification of Hyaluronidase Activity in Rabbit Dental Pulp, J Dent Res 60: 850-854.[Abstract/Free Full Text]
  • Silbert, J.E. (1982): Structure and Metabolism of Proteoglycans and Glycosaminoglycans, J Invest Derm 79:315-375.
  • Soru, E. and Ionescu-Stoian, F. (1965): The Purification of Testicular Hyaluronidase by Chromatography on a Mixed Column, J Chromatog 17:538-541.[CrossRef][Medline] [Order article via Infotrieve]
  • Swann, D.A. and Caulfield, J.B. (1975): Studies on Hyaluronic Acid. V. Relationship between the Protein Content and Viscosity of Rooster Comb Dermis Hyaluronic Acid, Connect Tissue Res 4:31-39.[CrossRef][Medline] [Order article via Infotrieve]
  • Tam, Y.C.; Harvey, R.F.; and Chan, E.C.S. (1982): Chondroitin Sulfatase-producing and Hyaluronidase-producing Oral Bacteria Associated with Periodontal Disease, J Can Dent Assoc 48:115-120.[Medline] [Order article via Infotrieve]
  • Tam, Y.C. and Chan, E.C.S. (1983): Phase Variation of Hyaluronidase-producing Peptostreptococci Associated with Periodontal Disease, J Dent Res 62:1009-1012.[Abstract/Free Full Text]
  • Toole, B.P. (1972): Hyaluronate Turnover during Chondrogenesis in the Developing Chick Limb and Axial Skeleton, Dev Biol 29: 321-329.[CrossRef][Medline] [Order article via Infotrieve]
  • Toole, B.P. and Gross, J. (1971): The Extracellular Matrix of the Regenerating Newt Limb: Synthesis and Removal of Hyaluronate Prior to Differentiation, Dev Biol 25:57-77.[Medline] [Order article via Infotrieve]
  • Toole, B.P. and Trelstad, R.L. (1971): Hyaluronate Production and Removal During Cornea Development in the Chick, Dev Biol 26:28-35.[CrossRef][Medline] [Order article via Infotrieve]
  • Tynelius-Bratthall, G. (1972): Hyaluronidase Activity in Gingival Crevice Material and in Peritoneal Exudate Leukocytes in Dogs, J Periodont Res 7:3-7.[CrossRef][Medline] [Order article via Infotrieve]
  • Van Palenstein Helderman, W.H. and Hoogeveen, L.C.M. (1976): Bacterial Enzymes and Viable Counts in Crevices of Noninflamed and Inflamed Gingiva, J Periodont Res 11: 25-34.[CrossRef][Medline] [Order article via Infotrieve]
  • Yamada, M.; Hasegawa, E.; and Kanamori, M. (1977): Purification of Hyaluronidase from Human Placenta, J Biochem 81:485-494.[Abstract/Free Full Text]
  • Yang, C.H. and Srivastava, P.N. (1974): Separation and Properties of Hyaluronidase from Rat Sperm Acrosomes, J Reprod Fert 37:17-25.[Abstract/Free Full Text]
  • Yang, C.H. and Srivastava, P.N. (1975): Purification of Bull Sperm Hyaluronidase by Concanavalin-A Affinity Chromatography, Biochem Biophys Acta 391:382-287.[Medline] [Order article via Infotrieve]
  • Zaneveld, L.J.D.; Polakoski, K.L.; and Schumacher, G.F.B. (1973): Properties of Acrosomal Hyaluronidase from Bull Spermatozoa: Evidence for its Similarity to Testicular Hyaluronidase, J Biol Chem 248:564-570.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 64, No. 6, 913-918 (1985)
DOI: 10.1177/00220345850640061101


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
JDRHome page
C.N. Bertolami, R.H. Day, and D.G. Ellis
Separation and Properties of Rabbit Buccal Mucosal Wound Hyaluronidase
Journal of Dental Research, June 1, 1986; 65(6): 939 - 944.
[Abstract] [PDF]


This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Bertolami, C.N.
Right arrow Articles by Ellis, D.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertolami, C.N.
Right arrow Articles by Ellis, D.G.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?