Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Baker, J.J.
Right arrow Articles by Tondreau, S.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baker, J.J.
Right arrow Articles by Tondreau, S.P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

The Stimulation of Human Peripheral Blood Lymphocytes by Oral Bacteria: Macrophage and T-Cell Dependence

J.J. Baker

Department of Microbiology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

S.P. Tondreau

Department of Cell Biology, Litton Bionetics, Kensington, Maryland 20795

Human peripheral blood mononuclear lymphocytes from individuals with moderate periodontitis were separated into purified subpopulations of T lymphocytes and B lymphocytes by rosetting with sheep red blood cells (E). All three lymphocyte subpopulations were compared for proliferative responses to cell walls from seven oral bacteria, phytohemagglutinin (PHA), pokeweed mitogen (PWM), lipopolysaccharide (LPS), and streptolysin-O (SLO). Mononuclear cells and are-combined subpopulation consisting of four parts purified T lymphocytes and one part B lymphocytes responded significantly to all of the stimulants. Purified T lymphocytes by themselves responded significantly to PHA and PWM, but were unresponsive to oral bacteria and SLO; however, T lymphocytes cultured with 2% autologous macrophages responded significantly to all seven oral bacterial cell walls and to SLO, which indicates that T-cell responses to oral bacteria are macrophage-dependent. T-cell-depleted non-E-rosette-forming B cells by themselves were poorly responsive to all of the tested stimulants; however, the responses of these cells to oral bacteria, PWM, LPS, and SLO increased significantly in the presence of 10% mitomycin-C-treated T cells, demonstrating that B cell proliferation to these stimulants is T-cell dependent.

REFERENCES

  • Baker, J.J.; Chan, S.P.; Socransky, S.S.; Oppenheim, J.J.; and Mergenhagen, S.E. (1976); Importance of Actinomyces and Certain Gram-Negative Anaerobic Organisms in the Transformation of Lymphocytes from Patients with Periodontal Disease, Infect Immun 13:1363-1368.[Abstract/Free Full Text]
  • Bick, P.H.; Carpenter, A.B.; Holdeman, L.V.; Miller, G.A.; Ranney, R.R.; Palcanis, K.G.; and Tew, J.G. (1981): Polyclonal B-Cell Activation Induced by Extracts of Gram-Negative Bacteria Isolated from Periodontally Diseased Sites, Infect Immun 34:43-49.[Abstract/Free Full Text]
  • Chen, P. and Doroszczak, N. (1980): Differences in Lymphoproliferative Responses to the Bacterium Actinomyces viscosus in Various Mammalian Species, Arch Oral Biol 27:319-324.[CrossRef]
  • Chen, P.; Trummel, C.; Horton, J.; Baker, J.J.; and Oppenheim, J.J. (1976): Production of Osteoclast Activating Factor by Normal Human Peripheral Blood Rosetting and Non-Rosetting Lymphocytes, Eur J Immunol 6:732-736.[CrossRef][Medline] [Order article via Infotrieve]
  • Cline, M.J. and Swett, V.C. (1968): The Interaction of Human Monocytes and Lymphocytes, J Exp Med 128:1309-1325.[Abstract/Free Full Text]
  • Devries, J.E.; Caviles, A.; Bont, W.S.; and Mendelsohn, J. (1979): The Role of Monocytes in Human Lymphocyte Activation by Mitogens, J Immunol 122:1099-1107.[Abstract/Free Full Text]
  • Donaldson, S.L.; Ranney, R.R.; Burmeister, J.A.; and Tew, J.G. (1982): Blastogenic Responses by Lymphocytes from Periodontally Healthy Populations Induced by Periodontitis-Associated Bacteria, J Periodontol 53:743-751.[Medline] [Order article via Infotrieve]
  • Donaldson, S.L.; Ranney, R.R.; and Tew, J.G. (1983): Evidence of Mitogenic Activity in Periodontitis-Associated Bacteria, Infect Immun 42:487-495.[Abstract/Free Full Text]
  • Dziarski, R.; Dziarski, A.; and Levinson, A.I. (1980): Mitogenic Responsiveness of Mouse, Rat and Human Lymphocytes to Staphylococcus aureus Cell Wall, Teichoic Acid and Peptidoglycan, Int Arch Allergy Appl Immunol 63:383-395.
  • Horton, J.E.; Leiken, S.;and Oppenheim, J.J. (1972): Human Proliferative Reaction to Saliva and Dental Plaque Deposits: An In Vitro Correlation with Periodontal Disease, J Periodontol 43: 522-527.[Medline] [Order article via Infotrieve]
  • Horton, J.E.; Oppenheim, J.J.; Chan, S.P.; and Baker, J.J. (1976): Relationship of Transformation of Newborn Human Lymphocytes by Dental Plaque Antigen to the Degree of Maternal Periodontal Disease, Cell Immunol 21:153-160.[CrossRef][Medline] [Order article via Infotrieve]
  • Horton, J.E.; Oppenheim, J.J.; and Mergenhagen, S.E. (1974): A Role for Cell-mediated Immunity in the Pathogenesis of Periodontal Disease, J Periodontol 45:351-360.[Medline] [Order article via Infotrieve]
  • Ivanyi, L. and Lehner, T. (1970): Stimulation of Lymphocyte Transformation by Bacterial Antigens in Patients with Periodontal Disease, Arch Oral Biol 15:1089-1096.[CrossRef][Medline] [Order article via Infotrieve]
  • Kiger, R.D.; Wright, W.H.; and Creamer, H.R. (1974): The Significance of Lymphocyte Transformation Response to Various Microbial Stimulants, J Periodontol 43:780-785.
  • Lang, N.P. and Smith, F.N. (1977): Lymphocyte Blastogenesis to Plaque Antigens in Human Periodontal Disease. I. Populations of Varying Severity of Disease, J Periodont Res 12:298-309.
  • Levinson, A.I.; Dziarski, A.; Zweiman, B.; and Dziarski, R. (1983): Staphylococcal Peptidoglycan: T-Cell-Dependent Mitogen and Relatively T-Cell-Independent Polyclonal B-Cell Activator of Human Lymphocytes, Infect Immun 39:290-296.[Abstract/Free Full Text]
  • Levis, W.R. and Robbins, J.H. (1977): Effect of Glass Adherent Cells on the Blastogenic Responses of Purified Lymphocytes to Phytohemagglutinin, Exp Cell Res 61:153-158.[CrossRef]
  • Lopatin, D.E.; Mangan, D.F.; and Horner, I.S. (1981): Cells Involved in the Mitogen-Induced Helper Function which Facilitates the Blastogenic Response to Actinomyces viscosus, Clin Immunol Immunopathol 19:394-405.[CrossRef][Medline] [Order article via Infotrieve]
  • Lopatin, D.E.; Mangan, D.F.; Horner, I.S.; and Peebles, F.L. (1980): Mitogen-Induced Amplification of Blastogenesis in Lipopolysaccharide-Precultured Lymphocytes, Infect Immun 29:512-519.[Abstract/Free Full Text]
  • Lopatin, D.E.; Peebles, F.L.; and Horner, I.S. (1980): The Influence of Mitogen Costimulation on the Human Lymphocyte Blastogenic Response to Actinomyces viscosus Ultrasonicates, Clin Immunol Immunopathol 16:75-83.[CrossRef][Medline] [Order article via Infotrieve]
  • Lopatin, D.E.; Peebles, F.L.; Woods, R.W.; and Syed, S.A. (1980): In Vitro Evaluation in Man of Immunostimulation by Subfractions of Actinomyces viscosus, Arch Oral Biol 25:23-29.[CrossRef][Medline] [Order article via Infotrieve]
  • Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275.[Free Full Text]
  • Mackler, B.F.; Altman, L.C.; Wahl, S.; Rosenstreich, D.L.; Oppenheim, J.J.; and Mergenhagen, S.E. (1974): Blastogenesis and Lymphokine Synthesis by T and B Lymphocytes from Patients with Periodontal Disease, Infect Immun 10: 844-850.[Abstract/Free Full Text]
  • Mangan, D.F.; Laughon, B.E.; Bower, B.; and Lopatin, D.E. (1982): In Vitro Lymphocyte Blastogenic Responses and Titers of Humoral Antibodies from Periodontitis Patients to Oral Spirochete Isolates, Infect Immun 37:445-451.[Abstract/Free Full Text]
  • Mangan, D.F. and Lopatin, D.E. (1981): In Vitro Stimulation of Immunoglobulin Production from Human Peripheral Blood Lymphocytes by a Soluble Preparation of Actinomyces viscosus, Infect Immun 31:236-244.[Abstract/Free Full Text]
  • Mangan, D.F.; Won, T.; and Lopatin, D.E. (1983): Nonspecific Induction of Immunoglobulin M Antibodies to Periodontal Disease-Associated Microorganisms after Polyclonal Human B-Lymphocyte Activation by Fusobacterium nucleatum, Infect Immun 41:1038-1045.[Abstract/Free Full Text]
  • Miller, R.A.; Gartner, S.; and Kaplan, H.S. (1978): Stimulation of Mitogenic Responses in Human Peripheral Blood Lymphocytes by Lipopolysaccharide: Serum and T Helper Cell Requirements, J Immunol 121:2160-2164.[Abstract/Free Full Text]
  • Page, R.C. and Schroeder, H.E. (1981): Current Status of the Host Response in Chronic Marginal Periodontitis, J Periodontol 52:477-491.[Medline] [Order article via Infotrieve]
  • Patters, M.R.; Chen, P.; McKenna, J.; and Genco, R.J. (1980): Lymphoproliferative Responses to Oral Bacteria in Humans with Varying Severities of Periodontal Disease, Infect Immun 28:777-784.[Abstract/Free Full Text]
  • Patters, M.R.; Genco, R.J.; Reed, M.J.; and Mashimo, P.A. (1976): Blastogenic Response of Human Lymphocytes to Oral Bacterial Antigens: Comparison of Individuals with Periodontal Disease to Normal and Edentulous Subjects, Infect Immun 14: 1213-1220.[Abstract/Free Full Text]
  • Roitt, I.M. and Lehner, T. (1980): Immunology of Oral Diseases, Oxford: Blackwell Scientific Publications, pp. 323-362.
  • Smith, S.P.; Bick, P.H.; Miller, G.A.; Ranney, R.R.; Rice, P.L.; Lalor, J.H.; and Tew, J.G. (1980): Polyclonal Activation: Severe Periodontal Disease in Young Adults, Clin Immunol Immunopathol 16:354-366.[CrossRef][Medline] [Order article via Infotrieve]
  • Stashenko, P. (1982): Regulatory Effect of Monocytes on T Cell Proliferative Responses to Oral Microbial Antigens, Infect Immun 38:938-947.[Abstract/Free Full Text]
  • Stashenko, P.; Resmini, L.M.; Haffajee, A.D.; and Socransky, S.S. (1983): T Cell Responses of Periodontal Disease Patients and Healthy Subjects to Oral Microorganisms, J Periodont Res 18:587-600.[CrossRef][Medline] [Order article via Infotrieve]
  • Suzuki, J.B.; Sims, T.J.; and Page, R.C. (1983): Effect of Factors other than Pathologic Status on Responsiveness of Peripheral Blood Mononuclear Cells from Patients with Chronic Periodontitis, J Periodontol 54:408-419.[Medline] [Order article via Infotrieve]
  • Tew, J.G.; Miller, G.A.; Greene, E.J.; Rice, P.L.; Jordan, W.P.; Palcanis, K.G.; and Ranney, R.R. (1981): Immunological Studies of Young Adults with Severe Periodontitis. II. Cellular Factors, J Periodont Res 16:403-416.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 64, No. 6, 906-912 (1985)
DOI: 10.1177/00220345850640061001
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
A. Mathur and B.S. Michalowicz
Cell-Mediated Immune System Regulation in Periodontal Diseases
Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(1): 76 - 89.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Baker, J.J.
Right arrow Articles by Tondreau, S.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baker, J.J.
Right arrow Articles by Tondreau, S.P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?