This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Jansen, J.A. Articles by Van Mullem, P.J. Search for Related Content PubMed PubMed Citation Articles by Jansen, J.A. Articles by Van Mullem, P.J. Social Bookmarking                         What's this?

Ultrastructural Study of Epithelial Cell Attachment to Implant Materials

Department of Dental Materials

J.R. De Wijn

Department of Dental Materials

J.M.L. Wolters-Lutgerhorst

Department of Oral Histology, Dental School, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands

P.J. Van Mullem

Department of Oral Histology, Dental School, University of Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands

Attachment of gingival tissues to the protruding post of an endosseous dental implant is of great importance for the prognosis of its clinical success. Little is known about the requirements an implant material must meet to secure a durable permucosal seal. The objective of this study, therefore, was to gain insight into the morphology of epithelial cell-implant interfaces for a number of well-known or potential implant materials.

Guinea pig epithelial cells were cultured on gold, titanium, carbon, hydroxylapatite, carbonate apatite, and modified polystyrene substrates. The metallic and carbon substrates were obtained by vapor-phase deposition of thin films on polystyrene carriers. This technique allowed for the preparation of ultra-thin sections for transmission electron microscopy containing substrate as well as the cells adhering to it. The cultures on apatite were decalcified prior to sectioning.

The results revealed that as attachment structures, focal contacts, extracellular matrix contacts, and hemidesmosome-like contacts could be distinguished. Hemidesmosome-like contacts were only observed on apatite and polystyrene and not on the metallic or carbon surfaces.

The results of this study suggest the existence of some, as yet unknown, property of the substrate that determines the nature and the structure of the contact site with epithelial cells.

REFERENCES

• Billig, D.; Nicol, A.; McGinty, R.; Cowin, P.; Morgan, J.; and Garrod, D. (1982): The Cytoskeleton and Substratum Adhesion in Chick Embryonic Corneal Epithelial Cells, J Cell Sci 57:51-71.[Medline] [Order article via Infotrieve]
• Chen, W.T. and Singer, S.J. (1982): Immunoelectron Microscopy Studies of the Sites of Cell-Substratum and Cell-Cell Contacts in Cultured Fibroblasts, J Cell Biol 95:205-222.[Abstract/Free Full Text]
• Cornell, R. (1969): Cell-Substrate Adhesion during Cell Culture, Exp Cell Res 58:289-295.[CrossRef][Medline] [Order article via Infotrieve]
• Culp, L.A. (1978): Biochemical Determinants of Cell Adhesion, Current Topics in Membranes and Transport 11:327-396.[CrossRef]
• Cranin, A.N.; Rabkin, M.F.; and Garfinkel, L.A. (1977): A Statistical Evaluation of 952 Endosteal Implants in Humans, JADA 94:315-320.
• Christophers, E. and Wolff, H.H. (1975): Differential Formation of Desmosomes and Hemidesmosomes in Epidermal Cell Cultures treated with Retinoic Acid, Nature 256:209-210.[CrossRef][Medline] [Order article via Infotrieve]
• Denissen, H.W. and De Groot, K. (1980): The Response of the Apatite Ceramic Surface to a Simulated Physiological Environment. In: Dental Implants, Materials and Systems, G. Heimke, Ed., München: Carl Hanser Verlag, pp. 35-40.
• Driessens, F.C.M.; Verbeeck, R.M.H.; and Heyligers, H.J.M. (1983): Some Physical Properties of Na- and CO3-containing Apatites Synthesized at High Temperatures, Inorgania ChimicaActa 80:19-23.
• Flaxman, B.A.; Lutzner, M.A.; and Van Scott, E.J. (1968): Ultrastructure of Cell Attachment to Substratum in vitro, J Cell Biol 36:406-410.[Free Full Text]
• Gould, T.R.L.; Brunette, D.M.; and Westbury, L. (1981): The Attachment Mechanism of Epithelial Cells to Titanium in vitro, J Periodont Res 16:611-616.[CrossRef][Medline] [Order article via Infotrieve]
• Harris, A. (1973): Location of Cellular Adhesions to Solid Substrate, Dev Biol 35:97-114.[CrossRef][Medline] [Order article via Infotrieve]
• James, R.A. and Schultz, R.L. (1974): Hemidesmosomes and the Adhesion of Junctional Epithelial Cells to Metal Implants, a Preliminary Report, Oral Implantol IV: 294-302.
• Jansen, J.A. (1981): Morphological Investigation of Cell Adhesion to Implant Materials (Morfologisch Onderzoek van Celadhesie aan Implantatiematerialen), Ned Tijdschrift Tandheelkunde 88: 437-439.
• Murray, J.C.; Stingl, G.; Kleinman, H.K.; Martin, G.R.; and Katz, S.I. (1979): Epidermal Cells Adhere Preferentially to Type IV (Basement Membrane) Collagen, J Cell Biol 80:197-202.[Abstract/Free Full Text]
• Ramaekers, F.C.S.; Puts, J.J.G.; Moesker, 0.; Kant, A.; Huysmans, A.; Haag, D.; Jap, P.H.K.; Herman, C.J.; and Vooijs, G.P. (1983): Antibodies to Intermediate Filament Proteins in the Immunohistochemical Identification of Human Tumors: An Overview, Histochem J 15:691-713.[CrossRef][Medline] [Order article via Infotrieve]
• Rosenberg, M.D. (1960): Microexudates from Cells Grown in Tissue Culture, Biophys J 1:137-159.[CrossRef][Medline] [Order article via Infotrieve]
• Sugimoto, A.; Rose, G.G.; Takarada, H.; and Cattori, M. (1973): Ultrastructural Changes of Basal Lamina and Anchoring Fibrils of Gingiva in vitro, J Periodont Res 7:127-142.
• Taylor, A.C. (1961): Attachment and Spreading of Cells in Culture, Exp Cell Res (Suppl) 8:154-173.[CrossRef]
• Taylor, A.C. (1970): Adhesion of Cells to Surfaces. In: Adhesion of Cells to Surfaces in Biological Systems, New York: Academic Press, pp. 51-71.

Journal of Dental Research, Vol. 64, No. 6, 891-896 (1985)
DOI: 10.1177/00220345850640060601


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. J. Pendegrass, D. Gordon, C. A. Middleton, S. Ng. M. Sun, and G. W. Blunn Sealing the skin barrier around transcutaneous implants: IN VITRO STUDY OF KERATINOCYTE PROLIFERATION AND ADHESION IN RESPONSE TO SURFACE MODIFICATIONS OF TITANIUM ALLOY J Bone Joint Surg Br, January 1, 2008; 90-B(1): 114 - 121. [Abstract] [Full Text] [PDF]

				K. A. Gross and C. C. Berndt Biomedical Application of Apatites Reviews in Mineralogy and Geochemistry, January 1, 2002; 48(1): 631 - 672. [Full Text] [PDF]

				J.A. Jansen, E.T. Den Braber, X.F. Walboomers, and J.E. De Ruijter Soft Tissue and Epithelial Models Advances in Dental Research, June 1, 1999; 13(1): 57 - 66. [Abstract] [PDF]

				R.M. Meffert Periodontitis vs. Peri-Implantitis: The Same Disease? The Same Treatment? Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(3): 278 - 291. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Jansen, J.A. Articles by Van Mullem, P.J. Search for Related Content PubMed PubMed Citation Articles by Jansen, J.A. Articles by Van Mullem, P.J. Social Bookmarking                         What's this?