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Effects of the Selective β2-Adrenergic Agonists, Procaterole and Terbutaline, on Protein Secretion by Rat Submandibular Glands

Department of Oral Biochemistry

H. Inoue

The First Department of Conservative Dentistry, Fukuoka Dental College, 700, Ta, Sawara-ku, Fukuokashi 814-01

Y. Yokota

Department of Oral Biochemistry, Gifu College of Dentistry, Gifu 501-02, Japan

We examined the secretory effects of two β₂-adrenergic agonists, procaterole and terbutaline, on the submandibular glands of anesthetized rats. After stimulation with these agents with and without a range of antagonists (non-specific - and β-adrenergic blockers), submandibular saliva was collected. The flow rate, protein concentration, the electrophoretic patterns, and amino acid composition of saliva were examined. These parameters were compared with their counterparts in saliva stimulated with isoproterenol (IPR), with and without antagonists. Assessed by these criteria, secreted proteins were classified as the - or β-type. In addition, IPR-stimulated proteins were compared in submandibular saliva of rats chronically treated with IPR or procaterole.

Both β₂-agonists were potent secretagogues for the submandibular glands of rats. All β-antagonists completely abolished the secretory effects elicited by both β₂-agonists, with the exceptions of carteolol and propranolol. However, no blocking agent abolished the secretory effects of IPR (60 mg/kg). The types of proteins in all submandibular saliva samples elicited by both β₂-agonists with and without antagonists were the β-type. Enlargement of the submandibular glands was not observed in rats subjected to chronic administration of procaterole, nor were abnormal and additional proteins observed, as confirmed by electrophoresis and by the amino acid analyses.

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Journal of Dental Research, Vol. 64, No. 6, 886-890 (1985)
DOI: 10.1177/00220345850640060501


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