Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Schwartz, J.
Right arrow Articles by Shklar, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwartz, J.
Right arrow Articles by Shklar, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Alpha Tocopherol Alters the Distribution of Langerhans Cells in DMBA-treated Hamster Cheek Pouch Epithelium

J. Schwartz

Harvard School of Dental Medicine, Department of Oral Pathology, 188 Longwood Avenue, Boston, Massachusetts 02115

O. Odukoya

Harvard School of Dental Medicine, Department of Oral Pathology, 188 Longwood Avenue, Boston, Massachusetts 02115

E. Stoufi

Harvard School of Dental Medicine, Department of Oral Pathology, 188 Longwood Avenue, Boston, Massachusetts 02115

G. Shklar

Harvard School of Dental Medicine, Department of Oral Pathology, 188 Longwood Avenue, Boston, Massachusetts 02115

Thirty-seven adult male and female golden hamsters (Mesocricetus auratus) were divided into four experimental groups. In Group A, the animals served as untreated controls, having the left buccal pouches painted with mineral oil. In Group B, the animals received 10 mg vitamin E (alpha tocopherol) in peanut oil by the oral route, with a fine pipette, twice weekly. In Group C animals, the left buccal pouch was painted three times weekly with DMBA (0.5% solution of 7,12 dimethylbenz(a)anthracene in heavy mineral oil). Group D animals received both vitamin E and DMBA in the amounts indicated for Groups Band C, with the vitamin E being administered on days alternate to the DMBA painting, also in the manner described for the above groups. All animals were killed after eight weeks of treatment. Epithelial whole mounts were prepared from the left buccal pouches. These specimens were then stained for ATPase to demonstrate the presence of Langerhans cells (LCs). A notably decreased density of LCs was observed after treatment with DMBA. Vitamin E administration in addition to DMBA treatment resulted in a less dramatic decrease in LC density. Since vitamin E has been shown to retard experimental oral carcinogenesis, vitamin E may retard carcinogenesis by maintaining the number of Langerhans cells.

REFERENCES

  • Axelrod, A.E. (1980): Nutrition in Relation to Immunity. In: Modem Nutrition in Health, and Disease, 6th ed. R.S. Goodhart and M.E. Shils, Eds. Philadelphia: Lea and Febiger, pp. 578-591.
  • Baehner, R.L. and Boxer, L.A. (1979): Role of Membrane Vitamin E and Cytoplasmic Glutathione in the Regulation of Phagocyte Function of Neutrophils and Monocytes, Amer J Ped Hemato Oncol 1:71-76.
  • Bailey, B.J.R. (1977): Tables of the Bonferroni and Statistics, J Amer Stat Assoc 72:358, 469-477.
  • Baernfiend, J.C.; Newmark, H.; and Brim, M. (1974): Vitamin A and E Nutrition via Intramuscular and Oral Route, Am J Clin Nutr 27:234-240.[Abstract]
  • Bliznakov, E.G. (1971): Protective Effect of Reticulo-endothelial System Stimulants in Combination with Chlorozine on Plasmodium berghei Infection in Mice. In: The Reticuloendothelial System and Immune Phenomena, M.R. Diluzio, Ed. New York: Plenum Press, pp. 315-323.
  • Bollag, W. (1972): Prophylaxis of Chemically-induced Benign and Malignant Epithelial Tumors by Vitamin A Acid (Retinoic Acid), Eur J Cancer 8:689-693.[Medline] [Order article via Infotrieve]
  • Breathnach, A.S.; Silvers, W.K.; Smith, J.; and Heyner, S. (1967): Langerhans Cells in Mouse Skin Experimentally Deprived of Neural Crest Component, J Invest Dermatol 50: 147-160.[Medline] [Order article via Infotrieve]
  • Cameron, A.T. and Metzer, S. (1937): The Effects of Certain Diets on the Production of Tar Carcinoma in Mice, Am J Cancer 30:70-74.
  • Campbell, P.A.; Cooper, H.R.; Heinzerling, R.H.; and Tengerdy, R.P. (1974): Vitamin E Enhances in vitro Immune Response by Normal and Non-adherent Spleen Cells, Proc Soc Exp Biol Med 146:465-469.[CrossRef][Medline] [Order article via Infotrieve]
  • Carruthers, C. (1939): Vitamin E and Experimental Tumors, Am J Cancer 35:546-553.
  • Cook, M.G. and McNamara, P. (1980): Effect of Dietary Vitamin E on Dimethyl-hydrazine-induced Colonic Tumors in Mice, Cancer Res 40:1329-1332.[Abstract/Free Full Text]
  • Davidson, J.D. (1934): An Attempt to Inhibit the Development of Tar-carcinomata in Mice, Can Med Assoc J 31:486-487.
  • Gross, S. (1979): Antioxidant Relationship Between Selenium-dependent Glutathione, Peroxidase, and Tocopherol, Amer J Ped Hemat Oncol 1:61-69.
  • Harman, D. (1969): Dibenzanthracene-induced Cancer. Inhibition Effect of Dietary Vitamin E, Clin Res 17:125-129.
  • Heinzerling, R.H.; Tengerdy, R.P.; Wick, L.L.; and Lueker, D.C. (1974): Vitamin E Protects Against Diplococcus Pneumonia Type I Infection, Infect Immun 10:1292-1301.[Abstract/Free Full Text]
  • Heinzerling, R.H. (1974): Effect of Vitamin E on Immunity, Ph.D. Dissertation, Colorado State University, Fort Collins, Colorado.
  • Juhlin, L. and Shelley, W.B. (1977): New Staining Technique for the Langerhans Cells, Acta Derm Venereol (Stockh) 57:289-296.[Medline] [Order article via Infotrieve]
  • Klareskog, L.; Tjernlund, W.M.; Forsum, U.; and Peter-Son, P.A. (1977): Epidermal Langerhans Cells Express la Antigens, Nature 268:248-249.[CrossRef][Medline] [Order article via Infotrieve]
  • Krivit, W. (1979): Hematologic Aspects of Vitamin E, Amer J Ped Hemat Oncol 1:59-60.
  • Miller, J.J. and Nossal, G.J.V. (1964): Antigens in Immunity. VI: The Phagocytic Reticulum of Lymph Node Follicle, J Exp Med 120:1075-1085.[Abstract/Free Full Text]
  • Nockels, G.F. (1978): Protective Effects of Supplemental Vitamin E Against Infection, Fed Proc 38:2134-2137.
  • Rowden, G.; Phillips, T.M.; and Delovitch, T.L. (1978): Expression of la Antigens by Murine Keratinizating Epithelial Langerhans Cells, Immuno Genetics 7:465-478.
  • Schwartz, J.L.; Solt, D.B.; Pappo, J.; and Weichselbaum, R. (1981): Distribution of Langerhans Cells in Normal and Carcinogen-treated Mucosa of Buccal Pouches of Hamsters, J Dermatol Surg Oncol 7:1005-1010.[Medline] [Order article via Infotrieve]
  • Sheffy, B.E. and Schultz, R.D. (1979): Influence of Vitamin E and Selenium on Immune Response Mechanisms, Fed Proc 38: 2139-2143.[Medline] [Order article via Infotrieve]
  • Shelley, W.B. and Juhlin, L. (1977): Selective Uptake of Contact Allergens by the Langerhans Cells, Arch Dermatol 113:187-192.[Abstract/Free Full Text]
  • Shklar, G. (1972): Experimental Oral Pathology in the Syrian Hamster, Prog Exper Tumor Res 16:518-538.
  • Shklar, G. (1982): Inhibition of Oral Mucosal Carcinogenesis by Vitamin E, J Natl Cancer Inst 68:791-797.[Medline] [Order article via Infotrieve]
  • Silberberg-Sinakin, I.G.; Thorbeck, J.; Baer, R.L.; Rosenthal, S.A.; and Berezowsky, V. (1976): Antigen-bearing Langerhans Cells in Skin, Dermal Lymphatics and Lymph Nodes, Cell Immunol 25:137-151.[Medline] [Order article via Infotrieve]
  • Slaga, T.J. and Bracken, W.M. (1977): The Effects of Antioxidants on Skin Tumor Initiation and Aryl Hydrocarbon Hydroxylase, Cancer Res 37:1631-1635.[Abstract/Free Full Text]
  • Stingl, G.; Katz, S.I.; Green, I.; and Shevach, E.M. (1980): The Functional Role of Langerhans Cells, J Invest Dermatol 74: 315-318.[CrossRef][Medline] [Order article via Infotrieve]
  • Tanaka, T.; Fujiwara, H.; and Torisu, M. (1979): Vitamin E and Immunity. I: Enhancement of Helper T Cell Activity, Immunol 38:727-732.[Medline] [Order article via Infotrieve]
  • Telford, I.R. (1949): The Effects of Hypo- and Hyper- Vitaminosis E in Lung Tumor Growth in Mice, NY Acad Sci 52:132-134.[CrossRef]
  • Tengerdy, R.P. (1978): Effect of Vitamin E on Disease Resistance and Immune Responses. In: Tocopherol, Oxygen and Biomembranes. C. deDuve and O. Hayashi, Eds., New York: Elsevier, pp. 191-200.
  • Tengerdy, R.P. and Brown, J.D. (1978): Effect of Vitamin E and Protection of Chickens Against E. coli Infection, Poultry Sci 54:1242-1247.
  • Tengerdy, R.P.; Heinzerling, R.H., and Nockels, C.F. (1972): Effect of Vitamin E on the Immune Response of Hypoxic and Normal Chickens, Infect Immun 5:987-993.[Abstract/Free Full Text]
  • Tengerdy, R.P.; Heinzerling, R.H.; Brown, G.L.; and Mathias, M.M. (1973): Enhancement of the Humoral Immune Response by Vitamin E, Intern Arch Allergy Appl Immun 44: 221-227.
  • Toews, G.B.; Bergstresser, P.R.; and Streilein, J.W. (1980): Epidermal Langerhans Cell Density Determines Whether Contact Hypersensitivity or Unresponsiveness Follows Skin Painting with DNFB, J Immunol 124:445-453.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 64, No. 2, 117-121 (1985)
DOI: 10.1177/00220345850640020401
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 International Journal of ToxicologyHome page
Final Report on the Safety Assessment of Tocopherol, Tocopheryl Acetate, Tocopheryl Linoleate, Tocopheryl Linoleate/Oleate, Tocopheryl Nicotinate, Tocopheryl Succinate, Dioleyl Tocopheryl Methylsilanol, Potassium Ascorbyl Tocopheryl Phosphate, and Tocophersolan
International Journal of Toxicology, November 1, 2002; 21(3_suppl): 51 - 116.
[Abstract] [PDF]

Home page
 JDRHome page
I. D. Mandel and G. Shklar
Development of Experimental Oral Carcinogenesis and its Impact on Current Oral Cancer Resecuch
Journal of Dental Research, December 1, 1999; 78(12): 1768 - 1772.
[PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Schwartz, J.
Right arrow Articles by Shklar, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwartz, J.
Right arrow Articles by Shklar, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?