Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gaffar, A.
Right arrow Articles by Moreno, E.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaffar, A.
Right arrow Articles by Moreno, E.C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Evaluation of 2-Phosphono-butane 1,2,4 Tricarboxylate as a Crystal Growth Inhibitor in vitro and in vivo

A. Gaffar

Colgate-Palmolive Research Center, 909 River Road, Piscataway, New Jersey 08854, Author to whom correspondence should be addressed

E.C. Moreno

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

The inhibitory activity of 2-phosphono-butane 1,2,4 tricarboxylic acid (PBTA) in the formation of hydroxyapatite (HA) was studied in vitro and in vivo. PBTA, at 4 ppm, inhibited the spontaneous formation of HA in vitro from a supersaturated solution. PBTA, at 2 ppm, completely inhibited the crystal growth of HA; at lower concentrations, a direct relationship was found between the reduction of the initial precipitation rates and PBTA concentrations in the solution.

The effects of PBTA on human dental enamel in vitro were also evaluated at pH 5.0 and 7.5. Equimolar levels of EDTA served as the controls. In comparison with EDTA, the dissolution of enamel induced by PBTA was negligible. One percent solution of PBTA was evaluated in a rat calculus assay. In comparison with a placebo solution, it significantly (p = 0.05) reduced calculus formation when applied topically. It was also tested against calculus formation in beagle dogs. A topical application once a day of a 1% solution at pH 7.0 reduced calculus formation by 84% for 16 weeks. Analysis of these data suggests that the agent effectively reduces calculus formation in vivo.

REFERENCES

  • Anerud, A.; Loë, H.; Boysen, H.; and Smith, H. (1979): The Natural History of Periodontal Disease in Man. Changes in Gingival Health and Oral Hygiene Before 40 Years of Age, J Periodont Res 14:526-540.[CrossRef][Medline] [Order article via Infotrieve]
  • Ainamo, J.; Barmps, D.; Beagrie, G.; Cutress, T.; Martin, J.; and Sardoinfirri, J.E. (1982): Development of
  • World Health Organization (WHO) Community Periodontal Index of Treatment Needs, Int Dent J 32:281-291.
  • Francis, M.D. (1962): Inhibition of Calcium Hydroxyapatite Crystal Growth by Polyphosphonate and Polyphosphates, Calcif Tissue Res 3:151-162.[CrossRef]
  • Gaffar, A.; Niles, H.P.; and Davis, C.B. (1981): Evaluation of an Oligomer or an Oligomer Plus Cetyl Pyridinium Chloride Against Plaque, Stain, Calculus and Gingivitis, J Dent Res 60: 1432-1438.
  • Gaffar, A.; Moreno, E.; Varughese, K.; and Kresak, M. (1982): In vitro Evaluation of EDITEMPA on Hydroxyapatite Formation and Its Effects on Dental Enamel, Calcif Tissue Int 34:508-516.
  • Gaffar, A.; Moreno, E.C.; Mühlemann, H.R.; and Niles, H.P. (1983): Effects of EDITEMPA on Dental Calculus and Caries Formation in vivo, Calcif Tissue Int 35:362-365.[CrossRef][Medline] [Order article via Infotrieve]
  • Glas, J.E. and Krasse, B. (1962): Biophysical Studies on Dental Calculus From Germ Free and Conventional Rats, Acta Odontol Scand 20:127-134.[CrossRef][Medline] [Order article via Infotrieve]
  • Hay, D.I.; Schluckebier, S.K.; and Moreno, E.C. (1982): Equilibrium Dialysis and Ultrafiltration Studies of Calcium and Phosphate Binding of Human Salivary Proteins. Implications for Salivary Supersaturation With Respect to Calcium Phosphate, Calcif Tissue Int 34:531-538.[CrossRef][Medline] [Order article via Infotrieve]
  • Jethi, R.K. and Wadkin, C.L. (1971): Studies of the Mechanism of Biological Calcification. II. Evidence for a Multi-step Mechanism for Calcification by Tendon Matrix, Calcif Tissue Res 7: 277-289.[CrossRef][Medline] [Order article via Infotrieve]
  • LeGeros, R.Z. and Shannon, I.L. (1979): The Crystalline Components of Dental Calculi: Human vs. Dog, J Dent Res 58:2371-2377.[Abstract/Free Full Text]
  • Moreno, E.C.; Zahradnik, R.T.; Glasman, A.; and Hwu, R. (1977): Precipitation of Hydroxyapatite From Dilute Solutions Upon Seeding, Calcif Tissue Res 24:47-57.[CrossRef][Medline] [Order article via Infotrieve]
  • Nicholson, C.R.; Taylor, G.R.; and Mellberg, J.R. (1974): An Automatic Device for Removing Enamel Layers by Dissolution, Caries Res 9:293-299.
  • Regolati, B.; Schmid, R.; and Mühlemann, H.R. (1970): The Effects of Diphosphonate, Pyrophosphate and Sodium Fluoride on Drinking Habits of Osborne-Mendel Rats, Helv Odont Acta 14:34-36.[Medline] [Order article via Infotrieve]
  • Schroeder, H.E. (1969): Formation and Inhibition of Dental Calculus. Berne, Switzerland: Verlag H. Huber.
  • Tatevossian, A. and Gould, C.T. (1976): Methods of Sampling and Analyzing the Aqueous Phase of Dental Plaque, Arch Oral Biol 21:313-317.[CrossRef][Medline] [Order article via Infotrieve]
  • Williams, G. and Sallis, J.D. (1979): Structure Activity Relationship of Inhibitors of Hydroxyapatite Formation, Biochem J 184:181-184.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 64, No. 1, 6-11 (1985)
DOI: 10.1177/00220345850640011201
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
H. C. Margolis
Edgard C. Moreno, PhD: A Tribute to His Major Contributions to Oral Biology Research
Journal of Dental Research, October 1, 1998; 77(10): 1767 - 1771.
[Abstract] [PDF]

Home page
 ADRHome page
A. Gaffar, R.Z. Legeros, R.J. Gambogi, and J. Affutto
Inhibition of Formation of Calcium Phosphate Deposits on Teeth and Dental Materials: Recent Advances
Advances in Dental Research, December 1, 1995; 9(4): 419 - 426.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gaffar, A.
Right arrow Articles by Moreno, E.C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaffar, A.
Right arrow Articles by Moreno, E.C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?