|
Sign In to gain access to subscriptions and/or personal tools.
|
The Effect of Malnutrition on Caries Development and Saliva Composition in the Rat
I. Johansson
National Caries Program, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205
T. Ericson
National Caries Program, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205
W. Bowen
National Caries Program, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205
M. Cole
National Caries Program, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205
The purpose of this study was to investigate the effect in rats of malnutrition on caries development and on some antibacterial substances in saliva. By gastric intubation, two groups of ten rats each were fed a liquid basic diet (100% diet), and two similar groups were fed the basic diet diluted with an equal volume of water to contain 50% of the nutrients of the basic diet (50% diet). One group on undiluted and one group on the diluted basic diet were offered sucrose as a supplement by mouth 17 times daily, using a programmed feeding machine, and the other two groups were offered starch in a similar manner. The extent and depth of the caries lesions were five times greater in the low-nourished (50% diet) sucrose group in comparison with the group given the undiluted diet supplemented with sucrose. The 50% diet group ate more frequently from the offered supplement. The 50% diet induced lower salivary lactoperoxidase activities, and there was an additive effect of starch as supplement. Higher concentrations of sIgA were found in the saliva of animals fed the 50% diet supplemented with sucrose than in those fed sucrose and the 100% diet. Significantly greater levels of sIgA and IgG were found in the saliva from the 50% diet-sucrose group than in the 50% diet-starch group. Plaque scores differed slightly but not significantly between the groups. The starch group receiving 100% diet showed a slightly lower level of S. mutans infection as compared with the 50% diet-starch group; the caries scores in the starch groups did not differ.
REFERENCES
- Alvarez, O. (1979): The Effect of Protein Malnutrition on Monkey Parotid Saliva. Proceedings, "Saliva and Dental Caries", Kleinberg, I., Ellison, S. A., and Mandel, I.D., Eds. Sp Supp, Microbiol Abst, pp. 123-136.
- Aponte-Merced, L. and Navia, J.M. (1980): Pre-eruptive Protein-energy Malnutrition and Acid Solubility of Rat Molar Enamel Surfaces, Arch Oral Biol 25:701-705.[CrossRef][Medline]
[Order article via Infotrieve]
- Astrom, S.; Arrhenius, E.K.; and Von Der Decken, A. (1981): Reduced Transcription Activity of Rat Liver Chromatin After Protein Restriction and Selective Digestion of Nuclei with Micrococcus Nuclease, J Nutr 111:1258-1264.[Abstract/Free Full Text]
- Bell, R.G.; Turner, K.J.; Gracey, M.; Suharjono; and Sunoto (1976): Serum and Small Intestinal Immunoglobulin Levels in Undernourished Children, Am J Clin Nutr 29:392-397.[Abstract/Free Full Text]
- Bowen, W.H.; Amsbaugh, S.M.; Monell-Torrens, S.; Brunelle, J.; Kuzmiak-Jones, H.; and Cole, M.F. (1980): A Method to Assess Cariogenic Potential of Food Stuffs, JADA 100:677-681.
- Carlsson, B.; Ahlstedt, S.; Hanson, L.-A.; Lidin-Jans-Son, B.; Lindblad, B.S.; and Sultana, R. (1976): Escherichia coli 0 Antibody Content in Milk from Healthy Swedish Mothers and Mothers from a very Low Socio-economic Group of a Developing Country, Acta Pediatr Scand 65:417-423.[Medline]
[Order article via Infotrieve]
- Chandra, R.K. (1972): Immunocompetence in Undernutrition, J Pediatr 81:1194-1200.[CrossRef][Medline]
[Order article via Infotrieve]
- Chandra, R.K. (1974): Rosette-forming T Lymphocytes and Cell Mediated Immunity in Malnutrition, Br Med J 3:608-609.[Abstract/Free Full Text]
- Chandra, R.K. (1975): Serum Complement and Immunoconglutinin in Malnutrition, Arch Dis Child 50:225-229.[Abstract/Free Full Text]
- Chandra, R.K. (1977): Lymphocyte Subpopulations in Human Malnutrition: Cytotoxic and Suppressor Cells, Pediatrics 59: 423-427.[Abstract/Free Full Text]
- Chandra, R.K. and Newberne, P.M. (1977): Nutrition, Immunity and Infection. New York: Plenum Press, pp. 89-104.
- Cooper, W.C.; Good, R.A.; and Mariani, T. (1974): Effects of Protein Insufficiency on Immune Responsiveness, Am J Clin Nutr 27:647-664.[Abstract]
- Deitchman, G.C.; Antal, M.; and Watson, R.R. (1980): Concentrations of Immunoglobulin G and Amylase in Saliva of Moderately Protein Undernourished Rats, J Food Sci 45:1092-1093.[CrossRef]
- Glick, P.L. and Rowe, D.J. (1981): Effects of Chronic Protein Deficiency on the Formation of the Rat Incisor Teeth, Arch Oral Biol 26:459-462.[CrossRef][Medline]
[Order article via Infotrieve]
- Hall, H.D.; Merig, J.J.; and Schneyer, C.A. (1967): Metrecal Induced Changes in Human Saliva, Proc Soc Exp Biol Med 124: 532-536.[CrossRef][Medline]
[Order article via Infotrieve]
- Johansson, I.; Ericson, Th.; and Steen, L. (1984): Studies on the Effect of Diet on Saliva Composition and Caries Development. I. The Effect of Fasting on Saliva Composition, J Nutr (in press).
- Keyes, P.H. (1959): Dental Caries in the Molar Teeth of Rats: A Method for Diagnosing and Scoring Several Types of Lesions Simultaneously, J Dent Res 37:1088-1099.
- Kolanowski, J. (1977): On the Mechanisms of Fasting Natriuresis and of Carbohydrate-induced Sodium Retention, Diabetes and Metabolism (Paris) 3:131-143.
- Konig, K.G.; Schmid, P.; and Schmid, R. (1968): An Apparatus for Frequency-controlled Feeding of Small Rodents and its Use in Dental Caries Experiments, Arch Oral Biol 13:13-26.[CrossRef][Medline]
[Order article via Infotrieve]
- Menaker, L. and Navia, J.M. (1973 a): Effect of Undernutrition during the Perinatal Period on Caries Development in the Rat. II. Caries Susceptibility in Underfed Rats Supplemented with Protein or Caloric Additions during the Suckling Period, J Dent Res 52:680-687.[Abstract/Free Full Text]
- Menaker, L. and Navia, J.M. (1973 b): Effect of Undernutrition during the Perinatal Period on Caries Development in the Rat: III. Effects of Undernutrition on Biochemical Parameters in the Developing Submandibular Salivary Gland, J Dent Res 52: 688-691.[Abstract/Free Full Text]
- Menaker, L. and Navia, J.M. (1974): Effect of Undernutrition during the Perinatal Period on Caries Development in the Rat: V: Changes in Whole Saliva Volume and Protein Content, J Dent Res 53:592-597.[Abstract/Free Full Text]
- Michalek, S.M.; Rahman, A.F.R.; and McGhee, J.R. (1975): Rat Immunoglobulins in Serum and Secretions: Comparison of IgM, IgA and IgG in Serum, Colostrum , Milk, and Saliva of Protein Malnourished and Normal Rats, Proc Soc Exp Biol Med 148:1114-1118.
- Morrison, M. (1963): Iodination of Tyrosine: Isolation of Lactoperoxidase (Bovine), J Biol Chem 238:653-657.[Free Full Text]
- McMurray, D.N.; Rey, H.; Casazza, L.J.; and Watson, R.R. (1977): Effect of Moderate Malnutrition on Concentrations of Immunoglobulins and Enzymes in Tears and Saliva of Young Colombian Children, Am J Clin Nutr 30:1944-1948.[Abstract/Free Full Text]
- Navia, J.M.; DI Orio, L.P.; Menaker, L.; and Miller, S. (1970): Effect of Undernutrition During the Perinatal Period on Caries Development in the Rat, J Dent Res 49:1091-1098.[Abstract/Free Full Text]
- Neumann, C.G.; Lawlor, G.J.; Stiehm, E.R.; Swendseid, M.E.; Newton, C.; Herbert, J.; Ammann, A.J.; and Jacob, M. (1975): Immunologic Responses in Malnourished Children, Am J Clin Nutr 28:89-104.[Free Full Text]
- Osserman, E.F. and Lawlor, D.P. (1966): Serum and Urinary Lysozyme (Muramidase) in Monocytic and Monomyelocytic Leukemia, J Exp Med 124:921-951.[Abstract]
- Scrimshaw, N.S.; Wilson, D.; and Bressani, R. (1960): Infection and Kwashiorkor, J Trop Pediatr 6:37-43.[Free Full Text]
- Scrimshaw, N.S. (1975): Interactions of Malnutrition and Infection: Advances in Understanding In Protein-calorie Malnutrition. Olson, R., Ed. New York: Academic Press, pp. 363-367.
- Shern, R.J.; Monell-Torrens, E.; Couet, K.M.; Kingman, A.; and Bowen, W.H. (1981): Post-mortem Evaluation of Dental Plaque in Rats. Proceedings, Symposium, "Animal Models in
- Cariology", Tanzer, J.M., Ed. Sp. Supp., Microbiol Abstr, pp. 215-221.
- Shaw, J.H. (1969): Influence of Marginal and Complete Protein Deficiency for Varying Periods during Reproduction on Growth, Third-molar Eruption, and Dental Caries in Rats, J Dent Res 48: 310-316.
- Spector, T. (1978): Refinement of the Coomassie Blue Method of Protein Quantitation, Analyt Biochem 86:142-146.[CrossRef][Medline]
[Order article via Infotrieve]
- Von Der Decken, A.; Astrom, S.; and Arrhenius, E.K. (1981): Increased Transcription Activity of Rat Liver Chromatin after Protein Restriction and Limited Digestion of Nuclei with Micrococcus Nuclease, J Nutr 111:1249-1257.[Abstract/Free Full Text]
- Watson, R.R.; Horton, R.G.; and Clinton, J.M. (1977): Suppression of Secretory IgA Antibodies in Protein Malnourished Guinea Pigs Following a Chlamydial Eye and Vaginal Infection, Fed Proc 36:1251.
- Watson, R.R.; Reyes, M.A.; and McMurray, D.N. (1978): Influence of Malnutrition on the Concentration of IgA, Lysozyme, Amylase and Aminopeptidase in Children's Tears , Proc Soc Exp Biol Med 157:215-219.[CrossRef][Medline]
[Order article via Infotrieve]
- Watson, R.R. and McMurray, D.N. (1979): The Effects of Malnutrition on Secretory and Cellular Immune Processes, Crit Rev Food Sci Nutr, Vol. 12, Cleveland, Ohio: CRC Press, Inc., pp. 113-159.
- Watson, R.R. and Antal, M. (1980): Effect of Moderate Chronic Protein Deficiency on Rat Salivary Components, J Nutr 110:771-777.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 64, No. 1,
37-43 (1985)
DOI: 10.1177/00220345850640010701
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
I. Johansson, M. Lenander-Lumikari, and A.-K. Saellstrom
Saliva Composition in Indian Children with Chronic Protein-Energy Malnutrition
Journal of Dental Research,
January 1, 1994;
73(1):
11 - 19.
[Abstract]
[PDF]
|
|
|
|
|
