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Oligosaccharide Structures of the Low-molecular-weight Salivary Mucin from a Normal Individual and one with Cystic Fibrosis
M.S. Reddy
Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 312 Cary Hall, Buffalo, New York 14214
M.J. Levine
Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 312 Cary Hall, Buffalo, New York 14214
A. Prakobphol
Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 312 Cary Hall, Buffalo, New York 14214
Studies were begun to compare the oligosaccharide structures of the low-molecular-weight mucin purified from the submandibular-sublingual saliva of a normal individual with that from one with cystic fibrosis. Following alkaline/borotritide cleavage, neutral and sialic acid-containing chains were purified by a combination of gel filtration, paper chromatography, and high-voltage paper electrophoresis. Oligosaccharides ranged in size from a disaccharide to a heptasaccharide. Approximately 80% of the oligosaccharides were GalB1, 3 GalNAc; Fuc 1,2GalB1,3GalNAc; and NeuAc2,3GalB1, 3GalNAc. The other structures were Fuc1,2GalB1,4(Fuc1,3)GlcNAcB1,6 (GalB1,3)GalNAc or GalB1,4(Fuc1,3)GlcNAcB1,6(Fuc1,2GalB1, 3)GalNAc; GalB1,4(Fuc1,3)GlcNAcB1,6(NeuAc2,3GalB1,3)GalNAc; and Fuc1,2GalB1,4(Fuc1,3)GlcNAcB1,6 (NeuAc2,3Gal-B1,3)GalNAc. There were no apparent qualitative differences in the neutral and sialic acid-containing units recovered from the normal and cystic fibrosis samples.
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Journal of Dental Research, Vol. 64, No. 1,
33-36 (1985)
DOI: 10.1177/00220345850640010601
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