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Fissure Removal and Needle Scraping for Evaluation of the Bacteria in Occlusal Fissures of Human Teeth

Oral Microbiology and Cariology Programs and Department of Operative Dentistry, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455

C.F. Schachtele

Oral Microbiology and Cariology Programs and Department of Operative Dentistry, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455

The bacterial counts obtained with a needle-scrape method for collecting plaque samples from human occlusal fissures with incipient caries were compared with the microflora remaining in the fissures as determined with a fissure removal method. Scraping of six fissures with a sterile needle recovered only 18.2% of the total recovered fissure flora. The needle-scrape method failed to detect specific cariogenic bacteria which were present in four of the fissures. Treatment of nine fissures with an antibacterial solution demonstrated that the needle method failed to detect viable bacteria in eight of the fissures which were subsequently shown to contain bacteria by fissure removal. The commonly used needle-scraping method does not appear adequate for studies on the effect of antimicrobial agents on fissure plaque.

REFERENCES

• Buchanan, R.E. and Gibbons, N.E.: Bergey's Manual of Determinative Bacteriology, 8th ed., Baltimore: Williams and Wilkins Co., 1974.
• Caufield, P.W. and Gibbons, R.J.: Suppression of Streptococcus mutans in the Mouths of Humans by a Dental Prophylaxis and Topically Applied Iodine, J Dent Res 58:1317-1326, 1979.[Abstract/Free Full Text]
• Fejerskov, 0.; Silness, J.; Karring, T.; and Loë, H.: The Occlusal Fissure of Unerupted Third Molars as an Experimental Caries Model in Man, Scand J Dent Res 84:142-149, 1976.[Medline] [Order article via Infotrieve]
• Gibbons, R.J.; DePaola, P.F.; Spinell, D.M.; and Skobe, Z.: Interdental Localization of Streptococcus mutans as Related to Dental Caries Experience, Infect Immun 9:481-488, 1974.[Abstract/Free Full Text]
• Gold, O.G.; Jordan, H.V.; and Van Houte, J.: A Selective Medium for Streptococcus mutans, Arch Oral Biol 18:1357-1364, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Ikeda, T.; Sandham, H.J.; and Bradley, E.L.: Changes in Streptococcus mutans and Lactobacilli in Plaque in Relation to the Initiation of Dental Caries in Negro Children, Arch Oral Biol 18:555-566, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Jordan, H.V. and DePaola, P.F.: Effect of Topically Applied 3% Vancomycin Gel on Streptococcus mutans on Different Tooth Surfaces, J Dent Res 53:115-120, 1974.[Abstract/Free Full Text]
• Jordan, H.V.; Krause, B.; and Moller, A.: A Method for Sampling Human Dental Plaque for Certain "Caries-inducing" Streptococci, Arch Oral Biol 13:919-927, 1968.[CrossRef][Medline] [Order article via Infotrieve]
• Loë, H.; Karring, J.; and Theilade, E.: An in vivo Model for the Study of the Microbiology of Occlusal Fissures, Caries Res 7:120-129,1973.[Medline] [Order article via Infotrieve]
• Loesche, W.J.; Rowen, J.; Straffon, L.H.; and Loos, P.J.: Association of Streptococcus mutans with Human Dental Caries, Infect Immun 11:1252-1260, 1975.[Abstract/Free Full Text]
• Loesche, W.J.; Syed, S.A.; Murray, RJ; and Mellberg, J.R.: Effect of Topically Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque II. Pooled Occlusal and Pooled Approximal Samples, Caries Res 9:139-155, 1975.[Medline] [Order article via Infotrieve]
• Loesche, W.J. and Straffon, L.H.: Longitudinal Investigation of the Role of Streptococcus mutans in Human Fissure Decay, Infect Immun 26:498-507, 1979.[Abstract/Free Full Text]
• Meiers, J.C.; Wirthlin, M.R.; and Shklair, I.L.: A Microbiological Analysis of Human Early Carious and Non-Carious Fissures, J Dent Res 61:460-464, 1982.[Abstract/Free Full Text]
• Meiers, J.C. and Schachtele, C.F.: The Effect of an Antibacterial Solution on the Microflora of Human Incipient Fissure Caries, J Dent Res 63:47-51, 1984.[Abstract/Free Full Text]
• Radike, A.W.: Criteria for Diagnosis of Dental Caries. In: Proceedings of the Conference on the Clinical Testing of Cariostatic Agents, Chicago: American Dental Association, 1972. Rogosa, M.; Mitchell, J.A.; and Wiseman, R.F.: A Selective Medium for the Isolation and Enumeration of Oral and Fecal Lactobacilli, J Bacteriol 62:132-133, 1951.[Free Full Text]
• Svanberg, M. and Westergren, G.: Effect of SnF2, Administered as Mouth Rinses or Topically Applied on Streptococcus mutans, Streptococcus sanguis and Lactobacilli in Dental Plaque and Saliva, Scand J Dent Res 91:123-129, 1983.
• Zybler, L.J. and Jordan, H.V.: Development of a Selective Medium for Detection and Enumeration of Actinomyces viscosus and Actinomyces naeslundii in Dental Plaque, J Clin Microbiol 15:253-259, 1982.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 63, No. 8, 1051-1055 (1984)
DOI: 10.1177/00220345840630080801


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Meiers, J.C. Articles by Schachtele, C.F. Search for Related Content PubMed PubMed Citation Articles by Meiers, J.C. Articles by Schachtele, C.F. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB COPPER(II) SULFATE COPPER, ELEMENTAL ETHANOL POTASSIUM IODIDE Social Bookmarking                         What's this?