|
Sign In to gain access to subscriptions and/or personal tools.
|
Basic Biological Sciences Effect of Eugenol on Respiration and Division in Human Pulp, Mouse Fibroblasts, and Liver Cells in vitro
W.R. Hume
The University of Adelaide, Department of Dentistry, Adelaide, South Australia 5000, Australia
Eugenol depressed cell respiration in homogenates of human dental pulp and in mouse fibroblast monolayers. The depression was concentration-dependent, with a threshold at about 10 -4M and a maximum at 10-3M in both preparations. Onset of the depression appeared to be rapid. The effects of variation in both duration and concentration of eugenol exposure on subsequent uptake of 3H-thymidine were examined in mouse fibroblast monolayers and human pulp explants. Fibroblasts survived short-term (up to 12 hr) exposure to 10-3M eugenol or less, but died after exposure to 10-3M for one day or more. The cells survived exposure to 10-4M for ten days, the longest period examined Human pulp maintained in tissue culture medium showed similar eugenol susceptibility. Analysis of these data, when coupled with those of previous studies on eugenol release from ZOE and diffusion through dentin, gives strong support for the concepts that: (1) the blandness of ZOE when applied to intact dentin is due to eugenol reaching the pulp in sub-toxic concentrations, and (2) the irritant effect of ZOE when applied directly to soft tissue is due to the development of concentrations of eugenol in tissue adjacent to ZOE sufficient to inhibit respiration and thus kill cells.
REFERENCES
- Beagrie, G.S.; Main, J.H.P.; and Smith, D.C. (1972): Inflammatory Reaction Evoked by Zinc Polyacrylate and Zinc Eugenate Cements. A Comparison, Br Dent J 132:351-357.[CrossRef][Medline]
[Order article via Infotrieve]
- Brodin, P. and Orstavik, D. (1982): Effects of Therapeutic and Pulp Protecting Materials on Nerve Transmission in vitro, Scand J Dent Res 91:46-50.
- Cleaver, J.E. (1967): Thymidine Metabolism and Cell Kinetics. In: North Holland Research Monographs: Frontiers of Biology, Vol. 6. Amsterdam: North-Holland, pp. 43-48.
- Cotmore, J.M.; Burke, A.; Lee, N.H.; and Shapiro, I.M. (1979): Respiratory Inhibition of Isolated Rat Liver Mitochondria by Eugenol, Arch Oral Biol 24:565-568.[CrossRef][Medline]
[Order article via Infotrieve]
- Craig, R. (1980): Restorative Dental Materials, 6th ed. St. Louis: Mosby, p. 443.
- Dewhirst, F.E. (1978): Eugenol, a Prototype Phenolic Prostaglandin Synthetase Activity Inhibitor, its Anti-inflammatory Activity, its Effect on Sheep Vesicular Gland Cyclooxygenase, and Structure-Activity Relationships for Cyclooxygenase Inhibition by Sixty-three Phenolic Compounds. Ph.D. Thesis, University of Rochester, Rochester, New York, pp. 94-95.
- Dewhirst, F.E. and Goodson, J.M. (1974): Prostaglandin Synthetase Inhibition by Eugenol, Guiacol and Other Dental Medicaments, J Dent Res 53(Suppl.):104.[Abstract/Free Full Text]
- Glass, R.L. and Zander, H.A. (1949): Pulp Healing, J Dent Res 28:97-107.[Free Full Text]
- Goldman, H.M. and Cohen, W.D. (1973): Periodontal Therapy, 5th ed. St. Louis: Mosby, p. 642.
- Hensten-Pettersen, A. and Helgeland, K. (1977): Evaluation of Biologic Effects of Dental Materials Using Four Different Cell Culture Techniques, Scand J Dent Res 85: 291-296.[Medline]
[Order article via Infotrieve]
- Hume, W.R. (1983): Effect of Eugenol on Constrictor Responses in Blood Vessels of the Rabbit Ear, J Dent Res 62:1013-1015. Hume, W.R. (1984): An Analysis of the Release and the Diffusion through Dentin of Eugenol from Zinc Oxide-Eugenol Mixtures, J Den t Res 63:881-884.
- Hume, W.R.; Veaco, N.; and Becker, R. (1982): Concentration-dependent Depression of Cellular Respiration by Eugenol, J Dent Res 61:202.
- Hume, W.R.; Yoon, N.A.; and Matsunaga, M.L. (1981): Comparison of Fluoride Toxicity between Periodontal Pathogenic Bacteria and Rabbit Gingiva, Arch Oral Biol 26:467-472.[CrossRef][Medline]
[Order article via Infotrieve]
- Imai, Y.; Watanabe, A.; Chang, P.-I.; and Masuhara, E. (1982): Evaluation of the Biologic Effects of Dental Materials Using a New Cell Culture Technique, J Dent Res 61:1024-1027.[Abstract/Free Full Text]
- Jones, P.A.; Taintor, J.F.; and Adams, A.B. (1979): Comparative Dental Material Cytotoxicity Measured by Depression of Rat Incisor Pulp Respiration, J Endo 5:48-55.[CrossRef]
- Kozam, G. (1977): The Effect of Eugenol on Nerve Transmission, Oral Surg 44:799-805.[CrossRef][Medline]
[Order article via Infotrieve]
- Lindqvist, L. and Otteskog, P. (1980): Eugenol: Liberation from Dental Materials and Effect on Human Diploid Fibroblast Cells, Scand J Dent Res 88:552-556.[Medline]
[Order article via Infotrieve]
- Newman, M.J.; Hulem, C.; Colgate, J.; and Anselmo, C. (1979): Antibacterial Susceptibility of Plaque Bacteria, J Dent Res 58:1722-1732.[Abstract/Free Full Text]
- Ozeki, M. (1975): The Effects of Eugenol on the Nerve and Muscle in Crayfish, Comp Biochem Physiol 50(C):183-191.
- Seltzer, S. and Bender, I.B. (1975): The Dental Pulp. Philadelphia: Lippincott, p. 266.
- Trowbridge, H.; Edwall, L.; and Panopoulos, P. (1982): Effect of Zinc Oxide-eugenol and Calcium Hydroxide on Intra-dental Nerve Activity, J Endo 8:403-406.[CrossRef]
- Wennberg, A. (1976): An in vitro Method for Toxicity Evaluation of Water Soluble Substances, Acta Odontol Scand 34:33-41.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 63, No. 11,
1262-1265 (1984)
DOI: 10.1177/00220345840630110101
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
M. A. Pogrel
Damage to the inferior alveolar nerve as the result of root canal therapy
J Am Dent Assoc,
January 1, 2007;
138(1):
65 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. R. Stanley
Trashing the Dental Literature-Misleading the General Practitioners: A Point of View
Journal of Dental Research,
September 1, 1996;
75(9):
1624 - 1626.
[PDF]
|
|
|
|
|
|
|
J.H. Jeng, L.J. Hahn, E. Lu, Y. Wang, and M.Y.P. Kuo
Eugenol Triggers Different Pathobiological Effects on Human Oral Mucosal Fibroblasts 1
Journal of Dental Research,
May 1, 1994;
73(5):
1050 - 1055.
[Abstract]
[PDF]
|
|
|
|
|
