|
Sign In to gain access to subscriptions and/or personal tools.
|
A Model for Producing Caries-like Lesions in Enamel and Dentin Using Oral Bacteria in vitro
B.H. Clarkson
Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242
J.S. Wefel
Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242
I. Miller
Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242
Subsurface enamel lesions and root surface caries-like lesions were consistently produced in vitro using Streptococcus mutans FA1 cultured in thioglycollate broth containing 3.5% w/v dextrose and 2% w/v gelatin. When viewed in polarized light and after imbibition in water, the enamel lesions had a negatively birefringent surface zone and positively birefringent body of the lesion. Those lesions produced after six weeks, after imbibition in quinoline, exhibited a dark zone. The root surface caries-like lesions exhibited a lessradiolucent surface zone above a heavily demineralized body of the lesion. However, no reactionary dentin was seen in the in vitro lesions.
REFERENCES
- Berkowitz, B.K.B.; Holland, G.R.; and Moxham, B.J. (1978): A Colour Atlas and Textbook of Oral Anatomy, London: Wolf Med Pub Ltd, pp. 79, 92, 197.
- Clarkson, B.H. and Silverstone, L.M. (1974): The Effects on Enamel of Several Different Fluoride Agents in vitro, J Int Assoc Dent Child 5:27-32.[Medline]
[Order article via Infotrieve]
- Dummer, P.M.H.; Edmunds, D.H.; and Green, R.M. (1982): Demineralization of Human Enamel by Streptococcus Mutans NCTC 10932 using a Sequential Batch Culture Technique, Caries Res 16:193-196.[Medline]
[Order article via Infotrieve]
- Feagin, F.F.; Clarkson, B.H.; and Wefel, J.S. (1984): Chemical and Physical Evaluation of Dialyzed-reconstituted Acidified Gelatin Surface Lesions of Human Enamel, Caries Res (in press).
- Fitzgerald, R.J.; Jordan, H.V.; and Stanley, H.R. (1960): Experimental Caries and Gingival Pathological Changes in the Gnotobiotic Rat, J Dent Res 39:923-935.[Free Full Text]
- Hardie, J.M.; Silverstone, L.M.; and Bowden, G.H. (1971): Modification of Acid Attack on Enamel Surface in vitro by Aggregations of Bacteria, Caries Res 5:190-304.
- Silverstone, L.M. (1966): The Primary Translucent Zone of Enamel Caries and of Artificial Caries-like Lesions, Br Dent J 120:461-471.[Medline]
[Order article via Infotrieve]
- Silverstone, L.M. (1967): Observations on the Dark Zone in Early Enamel Caries and in Artificial Caries-like Lesions, Caries Res 1:261-274.
- Silverstone, L.M. (1968): The Surface Zone in Caries and in Caries-like Lesions Produced in vitro, Br Dent J 125:145-157.[Medline]
[Order article via Infotrieve]
- Silverstone, L.M. (1977): Remineralization Phenomena, Caries Res 11 (Suppl. 1):59-84.[Medline]
[Order article via Infotrieve]
- Sumney, D.L. and Jordan, H.V. (1974): Characterization of Bacteria Isolated from Human Root Surface Caries, J Dent Res 53:343-351.[Abstract/Free Full Text]
- Von Ebner, V. (1894) (as quoted by W.J. Schmidt and A. Keil): Polarizing Microscopy of Dental Tissues. Oxford: Pergamon Press Ltd., 1971.
- Wefel, J.S. and Harless, J.D. (1984): Comparison of Artificial White Spots by Microradiography and Polarized Light Microscopy, J Dent Res (accepted).
- Zahradnik, R.T.; Propas, D.; and Moreno, E.C. (1977): In vitro Enamel Demineralization by Streptococcus mutans in the Presence of Salivary Pellicles, J Dent Res 56:1107-1110.[Abstract/Free Full Text]
Journal of Dental Research, Vol. 63, No. 10,
1186-1189 (1984)
DOI: 10.1177/00220345840630100201
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
A.S.M. Gilmour, D.H. Edmunds, and R.G. Newcombe
Prevalence and Depth of Artificial Caries-like Lesions Adjacent to Cavities Prepared in Roots and Restored with a Glass Ionomer or a Dentin-bonded Composite Material
Journal of Dental Research,
December 1, 1997;
76(12):
1854 - 1861.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W.M. Edgar and S.M. Higham
Role of Saliva in Caries Models
Advances in Dental Research,
November 1, 1995;
9(3):
235 - 238.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G.H. Bowden
The Role of Microbiology in Models of Dental Caries: Reaction Paper
Advances in Dental Research,
November 1, 1995;
9(3):
255 - 269.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A.R. Firestone, F.F. Feagin, T.J. Heaven, J. Sheetz, and F. Denys
In vitro Demineralization by Strains of Actinomyces viscosus and Streptococcus sobrinus of Sound and Demineralized Root Surfaces
Journal of Dental Research,
August 1, 1993;
72(8):
1180 - 1183.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F.A. Damato, R. Strang, and K.W. Stephen
Comparison of Solution- and Gel-prepared Enamel Lesions-an in vitro pH-cycling Study
Journal of Dental Research,
August 1, 1988;
67(8):
1122 - 1125.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D.G. Purton and J.C. Rodda
Artificial Caries around Restorations in Roots
Journal of Dental Research,
May 1, 1988;
67(5):
817 - 821.
[Abstract]
[PDF]
|
|
|
|
|
