Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kantor, M.L.
Right arrow Articles by Hassell, T.M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kantor, M.L.
Right arrow Articles by Hassell, T.M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Increased Accumulation of Sulfated Glycosaminoglycans in Cultures of Human Fibroblasts from Phenytoin-induced Gingival Overgrowth

M.L. Kantor

Department of Periodontics, School of Dentistry, and Dental Research Center, University of North Carolina, Chapel Hill, North Carolina 27514

T.M. Hassell

Department of Periodontics, School of Dentistry, and Dental Research Center, University of North Carolina, Chapel Hill, North Carolina 27514

Long-term ingestion of phenytoin elicits gingival overgrowth. We investigated sulfated glycosaminoglycan (GAG) metabolism by human gingival fibroblasts from normal gingivae and from phenytoin-enlarged gingivae. Incorporation, utilization, and subsequent loss of 35SO4= was measured in pulse-chase experiments. Fibroblasts from overgrown gingivae demonstrated increased accumulation of sulfated glycosaminoglycans. The increase was due to elevated synthesis and not to decreased degradation.

REFERENCES

  • Hassell, T.M.: Epilepsy and the Oral Manifestations of Phenytoin Therapy, Basel, Switzerland: Karger Publishers, 1981, pp. 122-123.
  • Hassell, T.M.; Page, R.C.; and Lindhe, J.: Histologic Evidence for Impaired Growth Control in Diphenylhydantoin Gingival Overgrowth in Man, Arch Oral Biol 23:381-384, 1978.[CrossRef][Medline] [Order article via Infotrieve]
  • Hall, B.K. and Squier, C.A.: Ultrastructural Quantitation of Connective Tissue Changes in Phenytoin-induced Gingival Overgrowth in the Ferret, J Dent Res 61:942-952, 1982.[Abstract/Free Full Text]
  • Hassell, T.M.: In vivo and in vitro Studies of the Pathogenesis of Phenytoin-induced Connective Tissue Alterations in the Gingivae. Doctoral Dissertation, University of Washington, Seattle, 1978.
  • Ballard, J.B. and Butler, W.T.: Proteins of the Periodontium. Biochemical Studies on the Collagen and Noncollagenous Proteins of Human Gingivae, J Oral Pathol 3:176-184,1974.[CrossRef][Medline] [Order article via Infotrieve]
  • Schnier, M.; Ogata, S.; and Fine, A.: Confirmation that Neither Phenotype nor Hydroxylation of Collagen is Altered in Overgrown Gingiva from Diphenylhydantoin Treated Patients, J Dent Res 57:506-510, 1978.[Abstract/Free Full Text]
  • Hassell, T.M.; Page, R.C.; Narayanan, A.S.; and Cooper, C.G.: Diphenylhydantoin (Dilantin) Gingival Hyperplasia: Drug-induced Abnormality of Connective Tissue, Proc Natl Acad Sci USA 73:2902-2912, 1976.
  • Benveniste, K. and Bitar, M.: Effects of Phenytoin on Cultured Human Gingival Fibroblasts. In: Phenytoin-Induced Teratology and Gingival Pathology, Hassell, T., Johnston, M., and Dudley, K., Eds., New York: Raven Press, 1980, pp. 199-214.
  • Hassell, T.M.: Evidence for Production of an Inactive Collagenase by Fibroblasts from Phenytoin-Enlarged Human Gingivae, J Oral Pathol 11:310-317, 1982.[CrossRef][Medline] [Order article via Infotrieve]
  • Hopkins, G. and Young, R.W.: Correlation of Microscopic with Instrumental Particle Counts, Bull Parenteral Drug Assoc 28:15-25, 1974.
  • Fratantoni, J.C.; Hall, C.W.; and Neufeld, E.F.: The Defect in Hurler's and Hunter's Syndromes: Faulty Degradation of Mucopolysaccharide, Proc Natl Acad Sci USA 60:699-706, 1968.[Free Full Text]
  • Lie, S.O.; McKusick, V.A.; and Neufeld, E.F.: Simulation of Genetic Mucopolysaccharidoses in Normal Human Fibroblasts by Alteration of pH of the Medium, Proc Natl Acad Sci USA 69:2361-2363, 1972.[Abstract/Free Full Text]
  • Bauer, E.A.; Fiehler, W.K.; and Esterly, N.B.: Increased Glycosaminoglycan Accumulation as a Genetic Characteristic in Cell Cultures of One Variety of Dominant Dystrophic Epidermolysis Bullosa, J Clin Invest 64:32-39, 1979.[CrossRef][Medline] [Order article via Infotrieve]
  • Jensen, S.H.; Page, R.C.; and Narayanan, A.S.: Effects of Plaque Accumulation on Gingival Collagen and Protein Production, J Dent Res 54:L4, 1975.
  • Greco, G.; Hassell, T.M.; and Walters, J.: Protein Synthesis by Fibroblasts from Healthy, Inflamed and Healed Human Gingiva from a Single Site, J Dent Res 61:274, 1982.
  • Narayanan, A.S.; Page, R.C.; and Kuzan, F.: Collagens Synthesized in vitro by Diploid Fibroblasts Obtained from Chronically Inflamed Human Connective Tissue, Lab Invest 39:61-65, 1978.[Medline] [Order article via Infotrieve]
  • Bauer, E.A. and Eisen, A.Z.: Recessive Dystrophic Epidermolysis Bullosa. Evidence tor Increased Collagenase as a Genetic Characteristic in Cell Culture, J Exp Med 148:1378-1387, 1978.[Abstract/Free Full Text]
  • Cohen, I.K.; Moore, C.D.; and Diegelmann, R.F.: Onset and Localization of Collagen Synthesis During Wound Healing in Open Rat Skin Wound, Proc Soc Exp Biol Med 160: 458-462, 1979.[CrossRef][Medline] [Order article via Infotrieve]
  • Matalon, R. and Dorfman, A.: Hurler's Syndrome: Biosynthesis of Acid Mucopolysaccharides in Tissue Culture, Proc Natl Acad Sci USA 56:1310-1316, 1966.[Free Full Text]
  • Matalon, R. and Dorfman, A.: The Accumulation of Hyaluronic Acid in Cultured Fibroblasts of the Marfan Syndrome, Biochem Biophys Res Commun 32:150-154, 1968.[CrossRef][Medline] [Order article via Infotrieve]
  • Buckingham, R.B.; Prince, R.K.; Rodnan, G.P.; and Taylor, F.: Increased Collagen Accumulation in Dermal Fibroblast Cultures from Patients with Progressive Systemic Sclerosis (Scleroderma), J Lab Clin Med 92:5-21, 1978.[Medline] [Order article via Infotrieve]
  • Herbert, C.M.; Jayson, M.I.V.; Lindberg, K.A.; and Bailey, A.J.: Biosynthesis and Maturation of Skin Collagen in Scleroderma, and Effect of D-penicillamine, Lancet 1:187-192,1974.[Medline] [Order article via Infotrieve]
  • LeRoy, E.C.: Connective Tissue Synthesis by Scleroderma Skin Fibroblasts in Cell Culture, J Exp Med 135:1351-1362, 1972.[Abstract]
  • LeRoy, E.C.: Increased Collagen Synthesis by Scleroderma Skin Fibroblasts in vitro. A Possible Defect in the Regulation or Activation of the Scleroderma Fibroblast, J Clin Invest 54: 880-889, 1974.[Medline] [Order article via Infotrieve]
  • Kresse, H.; Von Figura, K.; Buddecke, E.; and Fromme, H.G.: Metabolism of Sulfated Glycosaminoglycans in Cultivated Bovine Arterial Cells. I. Characterization of Different Pools of Sulfated Glycosaminoglycans, Hoppe-Seyler's Z Physiol Chem 356:929-941, 1975.[Medline] [Order article via Infotrieve]
  • Bashey, R.; Perlish, J.; Nochumson, S.; Stephens, R.; and Fleischmajer, R.: Connective Tissue Synthesis by Cultured Scleroderma Fibroblasts. II. Incorporation of 3H-Glucosamine and Synthesis of Glycosaminoglycans, Arthritis Rheum 20:879-885, 1977.[CrossRef][Medline] [Order article via Infotrieve]
  • Grossfield, H.; Meyer, K.; and Godman, G.: Differentiation of Fibroblasts in Tissue Culture, as Determined by Mucopolysaccharide Production, Proc Soc Exp Biol Med 88: 31-35, 1955.[CrossRef][Medline] [Order article via Infotrieve]
  • Enbery, G.; Oliver, W.; and Stanbury, J.: The Metabolism of Proteoglycans and Glycosaminoglycans in Inflamed Human Gingiva, J Periodont Res 14:512-519, 1979.[CrossRef][Medline] [Order article via Infotrieve]
  • Keech, M.K.: The Formation of Fibrils from Collagen Solutions. IV. Effect of Mucopolysaccharides and Nucleic Acids: An Electron Microscopic Study, J Biophys Biochem Cytol 9: 193-209, 1961.[Medline] [Order article via Infotrieve]
  • Toole, B.P. and Lowther, D.A.: The Effect of Chondroitin Sulfate-Protein on the Formation of Collagen Fibril in vitro, Biochem J 109:857-866, 1968.[Medline] [Order article via Infotrieve]
  • Obrink, B. and Sundelof, L.-O.: Light Scattering in the Study of Associating Macromolecules. The Binding of Glycosaminoglycans to Collagen, Eur J Biochem 37:226-232, 1973.[Medline] [Order article via Infotrieve]
  • Dziewiatkowski, D.; LaValley, S.; and LaValley, J.: Characterization of a Proteoglycan from Bovine Gingivae, J Dent Res 56:327, 1977.[Abstract/Free Full Text]
  • Wray, S. and Hassell, T.: Normal Gingivae Contain Functionally Different Fibroblast Subpopulations, J Dent Res 61:290,1982.
  • Hassell, T.M. and Stanek, E.J.: Evidence that Healthy Human Gingivae Contain Functionally Heterogeneous Fibroblast Subpopulations, Arch Oral Biol, In press.
  • Loë, H. and Silness, J.: Periodontal Disease in Pregnancy. I. Prevalence and Severity, Acta Odontol Scand 21:533-551, 1963.[Medline] [Order article via Infotrieve]
  • Mohlemann, H.R. and Son, S.: Gingival Sulcus Bleeding- A Leading Symptom in Initial Gingivitis, Helv Odont Acta 15:107-113, 1971.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 62, No. 3, 383-387 (1983)
DOI: 10.1177/00220345830620031601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
T. M. Hassell and A. F. Hefti
Drug-Induced Gingival Overgrowth: Old Problem, New Problem
Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(1): 103 - 137.
[Full Text] [PDF]

Home page
 Toxicol PatholHome page
T. Waner, A. Nyska, M. Nyska, M. Pirak, M. Sela, and A. Galiano
Gingival Hyperplasia in Dogs Induced by Oxodipine, a Calcium Channel Blocking Agent
Toxicol Pathol, April 1, 1988; 16(3): 327 - 332.
[Abstract] [PDF]

Home page
 Arch DermatolHome page
L. S. Moy, E. M. L. Tan, R. Holness, and J. Uitto
Phenytoin Modulates Connective Tissue Metabolism and Cell Proliferation in Human Skin Fibroblast Cultures
Arch Dermatol, January 1, 1985; 121(1): 79 - 83.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kantor, M.L.
Right arrow Articles by Hassell, T.M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kantor, M.L.
Right arrow Articles by Hassell, T.M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?