This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kashket, S. Articles by Ebersole, J.L. Search for Related Content PubMed Articles by Kashket, S. Articles by Ebersole, J.L. Social Bookmarking                         What's this?

The Effect of Prolonged Stimulation of Salivary Flow on Bacterial Reactive Factors

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

K.M. Guilmette

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

J.L. Ebersole

Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115

Prolonged stimulation of salivary flow leads to major changes in the concentrations of certain bacterial aggregating factors and strain-specific antibodies. Aggregating activity for a strain of Streptococcus sanguis was reduced rapidly, while activities for three strains of S. mutans remained unchanged, and activities for two other strains increased several-fold. The results were independent of changes in salivary protein and calcium. Concentrations of IgA and specific IgA antibodies for three S. mutans strains decreased progressively with continued stimulation. Similar results were obtained with parotid, submandibular, and whole saliva. These findings indicate that the rates of release of the different bacterial reactive substances from the salivary glands often follow independent courses and, furthermore, that the spectrum of reactivities of these substances changes with flow rate. Such changes may be reflected in the bacterial composition of plaque.

REFERENCES

• Dawes, C.: The Effects of Flow Rate and Duration of Stimulation on the Concentrations of Protein and the Main Electrolytes in Human Parotid Saliva, Arch Oral Biol 14:277-294, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Dawes, C.: The Effects of Flow Rate and Duration of Stimulation on the Concentrations of Protein and the Main Electrolytes in Human Submandibular Saliva, Arch Oral Biol 19:887-895, 1974.[CrossRef][Medline] [Order article via Infotrieve]
• Brandtzaeg, P.: Human Secretory Immunoglobulins - VII. Concentrations of Parotid IgA and Other Secretory Proteins in Relation to the Rate of Flow and Duration of Secretory Stimulus, Arch Oral Biol 16:1295-1310, 1971.[CrossRef][Medline] [Order article via Infotrieve]
• Mandel, I.D. and Khurana, H.S.: The Relation of Human Salivary Gamma-globulin and Albumin to Flow Rate, Arch Oral Biol 14:1433-1435, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Stuchell, R.N. and Mandel, I.D.: Studies of Secretory IgA in Caries-resistant and Caries-susceptible Adults, Adv Exp Med Biol 107:341-348, 1978.[Medline] [Order article via Infotrieve]
• Curby, W.A.: Device for Collection of Human Parotid Saliva, J Lab Clin Med 41:493-496, 1953.[Medline] [Order article via Infotrieve]
• Block, P. and Brotman, S.: A Method of Submaxillary Saliva Collection Without Cannulization, NY State Dent J 28: 116-118,1962.
• Kashket, S. and Liberman, E.: Fragility of Sailvary Aggregates of Streptococcus mutans, Caries Res 13:307-312, 1979.[Medline] [Order article via Infotrieve]
• Ericson, T.; Pruitt, K.; and Wedel, H.: The Reaction of Salivary Substances with Bacteria, J Oral Pathol 4:307-323, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Mancini, G.; Carbonara, A.D.; and Heremans, J.F.: Immunochemical Quantitation of Antigens by Single Radial Immunodiffusion, Immunochem 2:235-254, 1965.[CrossRef][Medline] [Order article via Infotrieve]
• Brandtzaeg, P.; Fjellanger, I.; and Gjeruldsen, S.T.: Human Secretory Immunoglobulins. I. Salivary Secretions from Individuals with Normal or Low Levels of Serum Immunoglobulins, Scand J Haematol Suppl. No. 12:1-83, 1970.
• Ebersole, J.L.; Frey, D.E.; Taubman, M.A.; and Smith, D.J.: An ELISA for Measuring Serum Antibodies to Actinobacillus actinomycetemcomitans, J Periodont Res 15: 621-632, 1980.[CrossRef][Medline] [Order article via Infotrieve]
• Itzhaki, R.F. and Gill, D.M.: A Micro-biuret Method for Estimating Proteins, Anal Biochem 9:401-410, 1964.[CrossRef][Medline] [Order article via Infotrieve]
• Lagerlof, F. and Ekstrand, J.: The Effect of Flow Rate on the Ionized Calcium Concentration of Human Parotid Saliva, Caries Res 16:123-128, 1982.[Medline] [Order article via Infotrieve]
• Kashket, S. and Donaldson, C.G.: Saliva-induced Aggregation of Oral Streptococci, J Bacteriol 112:1127-1133, 1972.[Abstract/Free Full Text]
• Magnusson, I.; Ericson, T.; and Pruitt, K.: Effect of Salivary Agglutinins on Bacterial Colonization of Tooth Surfaces, Caries Res 10:113-122, 1976.[Medline] [Order article via Infotrieve]
• Caldwell, R.C. and Pigman, W.: Changes in Protein and Glycoprotein Concentrations in Human Submaxillary Saliva under Various Stimulatory Conditions, Arch Oral Biol 11: 437-499, 1966.[CrossRef][Medline] [Order article via Infotrieve]
• Mandel, I.D.; Thompson, R.H., Jr.; and Ellison, S.A.: Studies on the Mucoproteins of Human Parotid Saliva, Arch Oral Biol 10:499-507, 1965.[CrossRef][Medline] [Order article via Infotrieve]
• Vaerman, J.P.: Comparative Immunochemistry of IgA, Res Immunochem Immunobiol 3:91-183, 1973.
• Kashket, S. and Guilmette, K.M.: Studies on a Human Salivary Glycoprotein with Specific Bacterial Adhesive Properties. In: Glycoconjugate Research, Vol. 1, Gregory, J. D. and Jeanloz, R.W., Eds., New York: Academic Press, Inc., 1979, pp. 529-532.
• Eggert, F.M.: The Nature of Secretory Agglutinins and Aggregating Factors. IV. Complexing Between Non-mucin Glycoproteins, Immunoglobulins and Mucins in Human Saliva and Amniotic Fluid, Int Arch Allergy Appl Immunol 62:46-58,1980.
• Van Houte, J. and Green, D.B.: Relationship Between the Concentration of Bacteria in Saliva and the Colonization of Teeth in Humans, Infect Immun 9:624-630, 1974.[Abstract/Free Full Text]
• Williams, R.C. and Gibbons, R.J.: Inhibition of Bacterial Adherence by Secretory IgA: A Mechanism of Antigen Disposal, Science 177:697-699, 1972.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 62, No. 3, 331-335 (1983)
DOI: 10.1177/00220345830620030301


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kashket, S. Articles by Ebersole, J.L. Search for Related Content PubMed Articles by Kashket, S. Articles by Ebersole, J.L. Social Bookmarking                         What's this?