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Fluoride Concentrations in Rat and Human Tooth Pulps and Their Possible Interference with Phosphatase Activities

Karolinska Institute, School of Dentistry, Department of Cariology and Laboratory of Oral Biochemistry, S - 103 64 Stockholm, Sweden

B. Angmar-Mansson

Karolinska Institute, School of Dentistry, Department of Cariology and Laboratory of Oral Biochemistry, S - 103 64 Stockholm, Sweden

Alkaline and acid phosphatases (p-ases), fluoride (F), and calcium (Ca) were analyzed in the pulps of rat incisors and young human pre-molars. P-ase activities were about ten times higher in the rat than in the human pulps; in the latter, high p-ase activity appeared to be connected with rapid mineralization. Average total F was 3-4 ng/mg in both rat and human pulp, while Ca showed values several times higher, although greatly varying, in human than in rat pulps. Ionized F was two to three times higher in ultrafiltrates from rat and human pulps than in the blood plasma of the species; in the rat, the F content of the ultrafiltrates increased only three to four times when plasma F was experimentally elevated over 100 times. In vitro, rat pulp alk. p-ase activity was slightly inhibited only in the range above 100 mM NaF, while acid p-ase showed weak inhibition in the region from 0.15-0.25 mM and 40-60% inhibition in the range from 10-100 mM NaF. In vivo, no effect on pulp p-ases was found, even from sublethal NaF doses.

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Journal of Dental Research, Vol. 62, No. 12, 1196-1200 (1983)
DOI: 10.1177/00220345830620120401


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ericsson, Y. Articles by Angmar-Mansson, B. Search for Related Content PubMed PubMed Citation Articles by Ericsson, Y. Articles by Angmar-Mansson, B. Social Bookmarking                         What's this?