Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Ranly, D.M.
Right arrow Articles by Lazzari, E.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ranly, D.M.
Right arrow Articles by Lazzari, E.P.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

A Biochemical Study of Two Bifunctional Reagents as Alternatives to Formocresol

D.M. Ranly

Department of Pediatric Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284

E.P. Lazzari

Department of Biochemistry, The University of Texas Dental Branch at Houston, Houston, Texas 77025

Two bifunctional reagents, glutaraldehyde and dimethylsuberimidate, were compared to formaldehyde with respect to fixation and modification of protein. Glutaraldehyde proved the superior fixative based on cross-linking and enzyme degradation assays. An immunoprecipitation assay suggests that the reaction products of glutaraldehyde may have altered antigenicity.

REFERENCES

  • Berger, J.E.: A Review of the Erroneously Labeled "Mummification" Techniques of Pulp Therapy, Oral Surg 34:131-144, 1972.[CrossRef][Medline] [Order article via Infotrieve]
  • Ranly, D.M. and Lazzari, E.P.: The Formocresol Pulpotomy - The Past, the Present and the Future, J Pedodont 2:115-127,1978.[Medline] [Order article via Infotrieve]
  • Rolling, I.; Hasselgran, G.; and Tronstad, L.: Morphologic and Enzyme Histochemical Observations on the Pulp of Human Primary Molars 3 to 5 Years after Formocresol Treatment, Oral Surg 42:518-528, 1976.[CrossRef][Medline] [Order article via Infotrieve]
  • Pashley, E.L.; Myers, D.R.; Pashley, D.H.; and Whit-Ford, G.M.: Systemic Distribution of 14C-formaldehyde from Formocresol-treated Pulpotomy Sites, J Dent Res 49: 603-608, 1980.
  • Block, R.M.; Lewis, R.D.; Sheaths, J.B.; and Fawley, J.: Cell-Mediated Immune Response to Dog Pulp Tissue Altered by Formocresol within the Root Canal, J Endo 3:424-430, 1977.[CrossRef]
  • Loos, P.J.; Straffon, L.H.; and Han, S.S.: Biologic Effects of Formocresol, J Dent Child 40: 193-197, 1973. 7. Morawa, A.P.; Straffon, L.H.; Han, S.S.; and Cor-Pron, R.E.: Clinical Evaluation of Pulpotomies Using Diluted Formocresol, J Dent Child 42:360-363, 1975.[Medline] [Order article via Infotrieve]
  • Lewis, B.B. and Chestner, S.B.: Formaldehyde in Dentistry: A Review of Mutagenic and Carcinogenic Potential, JADA 103:429-434, 1981.
  • s'Gravenmade, E.J.: Some Biochemical Considerations of Fixation in Endodontics, J Endo 1:233-237, 1974.
  • Nelson, J.R.; Lazzari, E.P.; Ranly, D.M.; and Madden, R.M.: Biochemical Effects of Tissue Fixatives on Bovine Pulp, J Endo 5:139-144, 1979.[CrossRef]
  • Davis, M.J.; Myers, R.; and Swetkes, M.D.: Glutaraldehyde an Alternative to Formocresol for Vital Pulp Therapy, J Dent Child 49:176-180, 1982.[Medline] [Order article via Infotrieve]
  • Kopel, H.M.; Bernick, S.; Zachrisson, E.; and De Romero, S.A.: The Effects of Glutaraldehyde on Primary Pulp Tissue Following Coronal Amputation: An in vivo Histologic Study, J Dent Child 47:425-430, 1980.[Medline] [Order article via Infotrieve]
  • Habeeb, A.F.S. A.: A Study of the Antigenicity of Formaldehyde and Glutaraldehyde-treated Bovine Serum Albumin and Ovalbumine-bovine Serum Albumin Conjugate, J Immunol 102:457-465, 1969.[Abstract/Free Full Text]
  • Dilley, G.J. and Courts, F.J.: Immunologic Response to Four Pulpal Medicaments, Pediatr Dent 3:179-183, 1981.[Medline] [Order article via Infotrieve]
  • Sawyer, N.A.: A Biochemical Study of the Effects of Formaldehyde, Glutaraldehyde and Diimidoesters on Bovine Pulp Tissue and Rabbit Albumin. Master of Science Thesis, University of Texas Dental Branch at Houston, June, 1982.
  • Dutton, A.; Adams, M.; and Singer, S.J.: Bifunctional Imidoesters as Cross-Linking Reagents, Biochem Biophys Res Commun 23:730-739, 1966.[CrossRef][Medline] [Order article via Infotrieve]
  • Hassel, J. and Hand, A.R.: Tissue Fixation with Diimidoesters as an Alternative to Aldehydes. 1. Comparison of Cross-linking and Ultrastructure Obtained with Dimethylsuberimidate and Glutaraldehyde, J Histochem Cytochem 22:223-239, 1974.[Abstract]
  • Maugh, T.H.: Sickle Cell (11): Many Agents Near Trials, Science 211:468-470, 1981.[Free Full Text]
  • Ranly, D.M.: Pulp Therapy in Primary Teeth. A Review and Prospectus, Acta Odontol Pediatr 3(2):63-68, 1982.[Medline] [Order article via Infotrieve]
  • Bradford, M.M.: A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal Biochem 72:248-254, 1976.[CrossRef][Medline] [Order article via Infotrieve]
  • Moore, S.: Amino Acid Analysis: Aqueous Dimethyl Sulfoxide as Solvent for the Ninhydrin Reaction, J Biol Chem 243:6281-6283, 1968.[Abstract/Free Full Text]
  • Ranly, D.M. and Fulton, R.: Reaction of Rat Molar Pulp Tissue to Formocresol, Formaldehyde, and Cresol, J Endo 2:176-181, 1976.
  • Mejare, I. and Larsson, AKE.: Short-Term Reactions of Human Dental Pulp to Formocresol and its Components - A Clinical Experimental Study, Scand J Dent Res 87:331-345, 1979.[Medline] [Order article via Infotrieve]
  • French, D. and Edsall, J.T.: The Reaction of Formaldehyde with Amino Acids and Proteins. In: Advances in Protein Chemistry, Vol. 2, Anson, M.L. and Edsall, J.T., Eds., New York, NY: Academic Press, Inc., 1945, pp. 278-336.
  • Stonehill, A.A.; Krop, S.; and Borick, P.M.: Buffered Glutaraldehyde - A New Chemical Sterilizing Solution, Am J Hosp Pharm 20:458-465, 1963.
  • Hunter, M.J. and Ludwig, M.L.: Amidination, Methods Enzymol 25:585-595, 1972.[CrossRef]
  • Ranly, D.M.: The Effect of pH and Concentration on the Glutaraldehyde Fixation of Bovine Pulp - An in vitro Study, Acta Odontol Pediat, Accepted for publication.

Journal of Dental Research, Vol. 62, No. 10, 1054-1057 (1983)
DOI: 10.1177/00220345830620100901


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
JDRHome page
D.M. Ranly, D. Horn, and T. Zislis
The Effect of Alternatives to Formocresol on Antigenicity of Proteins
Journal of Dental Research, October 1, 1985; 64(10): 1225 - 1228.
[Abstract] [PDF]


This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Ranly, D.M.
Right arrow Articles by Lazzari, E.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ranly, D.M.
Right arrow Articles by Lazzari, E.P.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?