Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Dahlén, G.
Right arrow Articles by Möller, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dahlén, G.
Right arrow Articles by Möller, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Production of Histolytic Enzymes by a Combination of Oral Bacteria with Known Pathogenicity

G. Dahlén

Department of Oral Microbiology, Institute of Medical Microbiology, University of Göteborg, Sweden

M. Wikström

Department of Oral Microbiology, Institute of Medical Microbiology, University of Göteborg, Sweden

Å. Möller

Department of Oral Microbiology, Institute of Medical Microbiology, University of Göteborg, Sweden

Eight oral bacterial strains, isolated from an infected root canal, have been investigated for their capacity to produce histolytic enzymes. The determination was performed using methods espoused by two different principles. Eleven out of 12 enzymes examined were demonstrated in the "eight-strain collection". In no single bacterial strain were all enzymes revealed. It was suggested that the pathogenicity of the bacterial strains, singly or in combination, was not solely dependent on the production of these enzymes. The histolytic enzymes may have a potentiating role on other pathogenic factors in infectious diseases.

REFERENCES

  • Kellner, A. and Robertsson, T.: Myocardial Necrosis Produced in Animals by Means of Crystalline Streptococcal Proteinase, J Exp Med 99:495, 1954.[Abstract]
  • Oakley, C.L.: Relative Toxicities and Assay Systems. In: Microbial Toxins, Vol. I, Ajl, S.J., Kadis, S., and Montie, T.C., Eds., New York: Academic Press, 1970, Chap. 10.
  • Davis, B.D.; Dulbecco, R.; Eisen, H.N.; Ginsberg, H.S.; and Wood, W.B.: Microbiology, New York: Harper and Row, 1970, Chap. 19.
  • van Heynigen, W.E.: In: Microbial Toxins, Vol. I, Ajl, S.J., Kadis, S., and Montie, T.C., Eds., New York: Academic Press, 1970, Chap. 1.
  • Gibbons, R.J. and McDonald, J.B.: Degradation of Collagen Substrates by Bacteroides melaninogenicus, J Bacteriol 81:614-621, 1961.[Free Full Text]
  • Rosan, B. and Williams, N.S.: Hyaluronidase Production of Oral Enterococci, Arch Oral Biol 9:291, 1964.[CrossRef]
  • Nord, C.E.: Purification and Properties of Hyaluronidase from Streptococcus mitis, Odontol Revy 21:1-12, 1970.[Medline] [Order article via Infotrieve]
  • Lederman, H.M.; Hayashi, J.A.; and Bahn, A.N.: Chondroitinsulfatase in Oral Bacteria, J Dent Res 51:206, 1972.[Free Full Text]
  • Soder, P.O. and Nord, C.E.: Determination of Hyaluronidase Activity in Dental Plaque Material, J Periodont Res 4: 208-214, 1969.[CrossRef][Medline] [Order article via Infotrieve]
  • Herson, L.E.: Elaboration of Hyaluronidase and Chondroitinsulphatase by Microorganisms Inhabiting the Gingival Sulcus. Evaluation of a Screening Method for Periodontal Disease, J Periodontol 42:34-36, 1971.[Medline] [Order article via Infotrieve]
  • Loesche, W.J.; Paunio, K.V.; Woolfolk, M.P.; and Hockett, R.N.: Collagenolytic Activity of Dental Plaque Associated with Periodontal Pathology, Infect Immun 9:329-336, 1974.[Abstract/Free Full Text]
  • Newman, M.G.: The Role of Bacteroides melaninogenicus and Other Anaerobes in Periodontal Infections, Rev Infect Dis 1:313-323,1979.[Medline] [Order article via Infotrieve]
  • Schultz-Haudt, S.D.; Dewar, M.; and Bibby, B.G.: Effects of Hyaluronidase in Human Gingival Epithelium, Science 117:653, 1953.[Free Full Text]
  • Smith, F.N. and Ramfjord, S.P.: Hyaluronidase Applied to the Gingiva of Rhesus Monkeys, J Periodontol 44:361-365, 1973.[Medline] [Order article via Infotrieve]
  • Barber, M.; Brooksbank, B.W.L.; and Kuper, S.W.A.: Staphylococcal Phosphatase, Glucuronidase and Sulphatase, J Pathol Bacteriol 63:57-64, 1951.[CrossRef][Medline] [Order article via Infotrieve]
  • Jacox, R.F.: Streptococcal β-glucuronidase, J Bact 65:700-705, 1953.[Free Full Text]
  • Lorina, P.L.; Lisanti, V.F.; and Chauncey, H.H.: The Production of β -glucuronidase and Hyaluronidase by Streptococcus mitis, Oral Surg 7:998-1010, 1954.[CrossRef][Medline] [Order article via Infotrieve]
  • Schultz-Haudt, S.D. and Scherp, M.W.: Production of Hyaluronidase and β-glucuronidase by Viridans Streptococci Isolated from Gingival Crevices, J Dent Res 34:924-929, 1960.
  • Fabricius, L.; Dahlen, G.; Holm, S.E.; and Möller, Å.J.R.: Influence of Combinations of Oral Bacteria on Periapical Tissues of Monkeys, Scand J Dent Res 90:200-206, 1982.[Medline] [Order article via Infotrieve]
  • Dahlén, G.; Fabricius, L.; Heyden, G.; Holm, S.E.; and Möller, Â.J.R.: Apical Periodontitis Induced by Selected Bacterial Strains in Root Canals of Immunized and Non-immunized Monkeys, Scand J Dent Res 90:207-216, 1982.[Medline] [Order article via Infotrieve]
  • Wikstrom, M.: Detection of Microbial Proteolytic Activity by a Cultivation Plate Assay in which Different Proteins Adsorbed to a Hydrophobic Surface are used as Substrates, Appl Environ Microbiol 45:393-400, 1983.[Abstract/Free Full Text]
  • Moller, Â.J.R.: Microbiological Examination of Root Canals and Periapical Tissues of Human Teeth,Thesis, Odontol Foren Tidskr (Spec Iss) 74:1-380, 1966.
  • Gallop, P.M. and Seifter, S.: Preparation and Properties of Soluble Collagens. In: Methods in Enzymology, Vol. VI, Colowick, S.P. and Kaplan, N.O., Eds., New York: Academic Press, 1963, pp. 635-641.
  • Bennick, A. and Hunt, A.M.: Collagenolytic Activity in Oral Tissues, Arch Oral Biol 12:1-9, 1967.[CrossRef][Medline] [Order article via Infotrieve]
  • Smith, R.F. and Willett, N.P.: Rapid Plate Method for Screening Hyaluronidase and Chondroitinsulfatase Producing Microorganisms, Appl Microbiol 16:1434-1436, 1968.[Medline] [Order article via Infotrieve]
  • Dahlen, G. and Linde, A.: Screening Plate Method for Detection of Bacterial β-glucuronidase, Appl Microbiol 26:863-866, 1973.[Medline] [Order article via Infotrieve]
  • Di Salvo, J.W.: Deoxyribonuclease and Coagulase Activity of Micrococci, Med Techns Bull Suppl to US Armed Forces Med Sci 9:191-196, 1958.
  • Ueno, K.; Fujii, H.; Marui, T.; Takamashi, J.; Sugi-Tani, T.; Ushijima, T.; and Suzuki, S.: Acid Phosphatase in Clostridium perfringens. A New Rapid and Simple Identification Method, Jpn J Microbiol 14:171-173, 1970.[Medline] [Order article via Infotrieve]
  • Dahlén, G. and Hofstad, T.: Endotoxic Activities of Lipopolysaccharides of Microorganisms Isolated from an Infected Root Canal in Macaca cynomolgus, Scand J Dent Res 85:272-278,1977.[Medline] [Order article via Infotrieve]
  • Edebo, L.: A New Press for the Disruption of Microorganisms and Other Cells, J Biochem Microbiol Technol Eng 2:453-479, 1960.[CrossRef]
  • Reissig, J.L.; Strominger, J.L.; and Leloir, L.F.: A Modified Colorimetric Method for the Estimation of N-acetylaminosugars, J Biol Chem 217:959-966, 1955.[Free Full Text]
  • Shuter, E.R.; Robins, E.; Freeman, M.L.; and Lun-Galwala, R.B.: β-Hexosaminidases in the Nervous System: The Quantitative Histochemistry of β-fucosaminidase and p-galactosaminidase in the Cerebral Cortex and Subjacent White Matter, J Histochem Cytochem 18:271-277, 1970.[Abstract]
  • Wikstrom, M.; Elwing, H.; and Moller, Å.J.R.: Proteins Adsorbed to a Hydrophobic Surface used for Determination of Proteolytic Activity, Enzyme Microbiol Technol 4:265-268, 1982.[CrossRef]
  • Vaes, G.: Lysosomes and the Cellular Physiology of Bone Resorption. In: Lysosomes in Biology and Pathology, Vol. 1, Dingle, J.T. and Fell, H.B., Eds., Amsterdam: North-Holland Publ. Co., 1968, Chap. 8, pp. 217-253.
  • Buchanan, R.E. and Gibbons, N.E.: Bergey's Manual of Determinative Bacteriology, 8th ed., Baltimore: Williams and Wilkins Company, 1974.
  • Holdeman, L.V. and Moore, W.E.C.: Anaerobic Laboratory Manual, 3rd ed., Blacksburg: Virginia Polytechnic Institute and State University, 1975.
  • Steffen, E.K. and Hentges, D.J.: Hydrolytic Enzymes of Anaerobic Bacteria Isolated from Human Infections, J Clin Microbiol 14:153-156, 1981.[Abstract/Free Full Text]
  • Linder, L. and Nord, C.E.: Hyaluronidase Activity in Cultures of an Anaerobe Corynebacterium and some Properties of the Enzyme, Scand J Dent Res 79:523-527, 1971.[Medline] [Order article via Infotrieve]
  • Puhvel, S.M. and Reisner, R.M.: The Production of Hyaluronidase (Hyaluronate Lyase) by Corynebacterium acnes, J Invest Dermatol 58:66-70, 1972.[CrossRef][Medline] [Order article via Infotrieve]
  • Porschen, R.K. and Spaulding, E.H.: Phosphatase Activity of Anaerobic Microorganisms, Appl Microbiol 27:744-747, 1974.[Medline] [Order article via Infotrieve]
  • Porschen, R.K. and Sonntag, S.: Extracellular Deoxyribonuclease Production by Anaerobic Bacteria, Appl Microbiol 27:1031-1033, 1974.[Medline] [Order article via Infotrieve]
  • McDougall, W.A.: The Effect of Topical Antigen on the Gingiva of Sensitized Rabbits, J Periodont Res 9:153-164, 1974.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 62, No. 10, 1041-1044 (1983)
DOI: 10.1177/00220345830620100601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Dahlén, G.
Right arrow Articles by Möller, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dahlén, G.
Right arrow Articles by Möller, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?