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Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

Y.H. Liau

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

A. Slomiany

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

B.L. Slomiany

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3'-phosphoadenosine-5'-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F¹-.

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Journal of Dental Research, Vol. 62, No. 10, 1026-1030 (1983)
DOI: 10.1177/00220345830620100201


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Zdebska, E. Articles by Slomiany, B.L. Search for Related Content PubMed PubMed Citation Articles by Zdebska, E. Articles by Slomiany, B.L. Social Bookmarking                         What's this?