|
Sign In to gain access to subscriptions and/or personal tools.
|
Pseudocholinesterase Activity and Its Origin in Human Oral Fluid
R. Ryhanen
Department of Physiology, University of Kuopio, POB 138, SF-70101 Kuopio 10, Finland
M. Narhi
Department of Physiology, University of Kuopio, POB 138, SF-70101 Kuopio 10, Finland
E. Puhakainen
Department of Physiology, University of Kuopio, POB 138, SF-70101 Kuopio 10, Finland
O. Hanninen
Department of Physiology, University of Kuopio, POB 138, SF-70101 Kuopio 10, Finland
V. Kontturi-Narhi
Department of Physiology, University of Kuopio, POB 138, SF-70101 Kuopio 10, Finland
Pseudocholinesterase (PCE) activity in oral fluid of 31 male and 24 female subjects was determined using butyrylthiocholine iodide as substrate. Males had approximately twice as much salivary PCE activity as did females [4.8 ± 2.4 (S.D.) U/1 and 2.2 ± 1.5 (S.D.) U/1, respectively]. The activity was not much affected by salivary flow rate, although in men it was a little higher in stimulated than in unstimulated saliva. Salivary PCE activity showed diurnal variation. Accordingly, activities were about three times greater at four a.m. than at four p.m. Parotid PCE activity correlated with that of whole saliva in both men and women. PCE activity in crevicular fluid (four subjects) was 120 ± 48 (S.D.) U/1. An elevation of PCE activity in oral fluid was found after experimental induction of gingival inflammation. However, the mean PCE activity of patients with clinical gingivitis was not significantly higher than that of healthy subjects, although some exceptionally high values were found. Sonicated samples of plaque did not contain any PCE activity. No correlation existed between PCE activities in saliva and serum.
REFERENCES
- Foldes, F.F. and Smith, J.C.: The Interaction of Human Cholinesterases with Anticholinesterases Used in the Therapy of Myasthenia Gravis, Ann NY Acad Sci 135:287-301, 1966.[CrossRef][Medline]
[Order article via Infotrieve]
- Witter, R.F.: Measurement of Blood Cholinesterase, Arch Environ Health 6:537-563, 1963.
- Chauncey, H.H.; Lionetti, F.; Winer, R.A.; and Lisan-Ti, V.F.: Enzymes of Human Saliva, the Determination, Distribution and Origin of Whole Saliva Enzymes , J Dent Res 33:321-334, 1954.[Medline]
[Order article via Infotrieve]
- Ueda, K. and Yamaguchi, K.: Cholinesterase Activity of Human Saliva and Types of the Enzymes: Comparison of Whole Saliva with Parotid Saliva, Bull Tokyo Dent Coll 17: 231-241, 1976.[Medline]
[Order article via Infotrieve]
- Lashley, K.S.: Reflex Secretion of the Human Parotid Gland, J Exp Psychol 1:461, 1916.[CrossRef]
- Holm-Pedersen, P.; Agerbaek, N.; and Theilade, E.: Experimental Gingivitis in Young and Elderly Individuals, J Clin Periodontol 2:14-24, 1975.[CrossRef][Medline]
[Order article via Infotrieve]
- Sueda, T.; Bang, J.; and Cimasoni, G.: Collection of Gingival Fluid for Quantitative Analysis, J Dent Res 48:159, 1969.[Free Full Text]
- Ellman, G.L.; Courtney, D.K.; Andres, V.; and Fea-Therstone, R.M.: A New and Rapid Colorimetric Determination of Acetylthiocholinesterase Activity, Biochem Pharmacol 7:88-95, 1961.[CrossRef][Medline]
[Order article via Infotrieve]
- Augustinsson, K.-B.; Eriksson, H.; and Faijersson, Y.: A New Approach to Determining Cholinesterase Activities in Samples of Whole Blood, Clin Chim Acta 89:239-252, 1978.[CrossRef][Medline]
[Order article via Infotrieve]
- Puhakainen, E.; Ryhanen, R.; and Penttila, I.: Serum Pseudocholinesterase and HDL-cholesterol, J Clin Chem Clin Biochem 18:684, 1980.
- Aoki, K., and Narita, T.: Discharge of Cholinesterase in Parotid Glandular Cells of Rats into Saliva, Kokukagaku-kai Zasshi 21:368-369, 1972.
- Juul, P.: Human Plasma Cholinesterase Isoenzymes, Clin Chim Acta 19:205-213, 1968.[CrossRef][Medline]
[Order article via Infotrieve]
- Lindqvist, L. and Augustinsson, K.-B.: Esterase Patterns in Human Saliva Established by Chromatography on Con-A-Sepharose and by Isoelectric Focusing, Arch Oral Biol 25:363-368, 1980.[CrossRef][Medline]
[Order article via Infotrieve]
- Henriques, B.L. and Chauncey, H.H.: Comparative Enzyme Concentrations of Submaxillary and Parotid Salivas, J Dent Res 38:733, 1959.
- Loë, H. and Holm-Pedersen, H.: Absence and Presence of Fluid from Normal and Inflamed Gingivae, Periodontics 3: 171-177,1965.
- Chauncey, H.H.: Salivary Enzymes, JADA 63:360-368, 1961.
- Lindqvist, L.; Nord, C.-E.; and Soder, P.-O.: Origin of Esterases in Human Whole Saliva, Enzyme 22:166-175, 1977.[Medline]
[Order article via Infotrieve]
- Vorhaus, L.J. and Kark, R.M.: Serum Cholinesterase in Health and Disease, Am J Med 14:707-719, 1953.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 62, No. 1,
20-23 (1983)
DOI: 10.1177/00220345830620010501
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
L. J. Dawson, V. L. Caulfield, J. B. Stanbury, A. E. Field, S. E. Christmas, and P. M. Smith
Hydroxychloroquine therapy in patients with primary Sjogren's syndrome may improve salivary gland hypofunction by inhibition of glandular cholinesterase
Rheumatology,
April 1, 2005;
44(4):
449 - 455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yamalik, N. Ozer, F. Caglayan, G. Caglayan, and T. Akdoganli
The Effect of Periodontal Therapy on Salivary Pseudocholinesterase Activity
Journal of Dental Research,
June 1, 1991;
70(6):
988 - 990.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. Yamalik, N. Ozer, F. Caglayan, and G. Caglayan
Determination of Pseudocholinesterase Activity in the Gingival Crevicular Fluid, Saliva, and Serum from Patients with Juvenile Periodontitis and Rapidly Progressive Periodontitis
Journal of Dental Research,
January 1, 1990;
69(1):
87 - 89.
[Abstract]
[PDF]
|
|
|
|
|
