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Molecular Composition of the Protein Matrix of Developing Human Dental Enamel

Skeletal Matrix Biochemistry Section, Laboratory of Biological Structure, National Institutes of Health, National Institute of Dental Research, Bethesda, Maryland 20205

A.B. Belcourt

Skeletal Matrix Biochemistry Section, Laboratory of Biological Structure, National Institutes of Health, National Institute of Dental Research, Bethesda, Maryland 20205

J.D. Termine

Skeletal Matrix Biochemistry Section, Laboratory of Biological Structure, National Institutes of Health, National Institute of Dental Research, Bethesda, Maryland 20205

The protein matrix of fetal human dental enamel was isolated and fractionated by chromatographic and electrophoretic procedures. Nine principal protein fractions were isolated and characterized. Possible inter-relationships of these proteins and their comparison with data from other species were examined. Significant differences between human proteins and those described for cow or pig enamel were identified.

REFERENCES

• Deakins, M.: Changes in Ash, Water and Organic Content of Pig Enamel During Calcification, J Dent Res 21:429-436, 1942.[Free Full Text]
• Eastoe, J.E.: Enamel Protein Chemistry - Past, Present and Future, J Dent Res 58B:753-763, 1979.[Abstract/Free Full Text]
• Termine, J.D.; Belcourt, A.B.; Christner, P.J.; Conn, K.M.; and Nylen, M.U.: Properties of Dissociatively Extracted Fetal Tooth Matrix Proteins. I. Principal Molecular Species in Developing Bovine Enamel, J Biol Chem 255:9760-9768, 1980.
• Belcourt, A.B.; Fincham, A.G.; and Termine, J.D.: Ethylenediaminetetraacetic Acid Insoluble Protein of Adult Human Enamel, Caries Res 16:72-76, 1982.[Medline] [Order article via Infotrieve]
• Robinson, C.; Briggs, H.D.; Atkinson, P.J.; and Weatherell, J.A.: Matrix and Mineral Changes in Developing Enamel, J Dent Res 58B:871-880, 1979.[Abstract/Free Full Text]
• Fincham, A.G.; Belcourt, A.B.; and Termine, J.D.: Changing Patterns of Enamel Matrix Proteins in the Developing Bovine Tooth, Caries Res 16:64-71, 1982.[Medline] [Order article via Infotrieve]
• Katz, E.P.; Mechanic, G.L.; and Glimcher, M.J.: The Ultracentrifugal and Free Zone Electrophoretic Characterization of the Neutral Soluble Proteins of Embryonic Bovine Enamel, Biochim Biophys Acta 107:471-484, 1965.[Medline] [Order article via Infotrieve]
• Burgess, R.C. and Maclaren, C.M.: Proteins in Developing Bovine Enamel. In: Tooth Enamel I, Stack, M.V. and Fearnhead, R.W., Eds., Bristol, England: Wright Bros., 1965, pp. 74-82.
• Fincham, A.G.: Electrophoretic and Sephadex Gel Filtration Studies of Bovine Fetal Enamel Matrix at Acid pH, Calcif Tissue Res 2:353-360, 1968.[CrossRef][Medline] [Order article via Infotrieve]
• Eggert, F.M.; Allen, G.A.; and Burgess, R.C.: Amelogenins - Purification and Partial Characterization of Proteins from Developing Bovine Dental Enamel, Biochem J 131:471-484, 1973.[Medline] [Order article via Infotrieve]
• Fukae, J. and Shimizu, M.: Studies on the Proteins of Developing Bovine Enamel, Arch Oral Biol 19:381-386, 1974.[CrossRef][Medline] [Order article via Infotrieve]
• Seyer, J. and Glimcher, M.J.: Evidence for the Presence of Numerous Protein Components in Immature Bovine Dental Enamel, Calcif Tissue Res 24:253-257, 1977.[CrossRef][Medline] [Order article via Infotrieve]
• Fincham, A.G.: The Amelogenin Problem: A Comparison of Purified Enamel Matrix Proteins, Calcif Tissue Intl 27:65-73, 1979.[CrossRef][Medline] [Order article via Infotrieve]
• Fukae, M.; Ijiri, H.; Tanabe, T.; and Shimizu, M.: Partial Amino Acid Sequences of Two Proteins in Developing Porcine Enamel, J Dent Res 58B:1000-1001, 1979.[Abstract/Free Full Text]
• Fukae, M.; Tanabe, T.; Ijiri, H.; and Shimizu, M.: Studies of Porcine Enamel Proteins: A Possible Original Enamel Protein, Tsurumi Univ Dent J 6:87-94, 1980.
• Hiller, C.R.; Robinson, C.; and Weatherell, J.A.: Variations in the Composition of Developing Rat Incisor Enamel, Calcif Tissue Res 18:1-12, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Elwood, W.K. and Apostopoulos, A.X.: Analysis of Developing Enamel of the Rat. I. Fractionation: Protein and Calcium Content, Calcif Tissue Res 17:317-326, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Levine, P.T.; Seyer, J.; Huddleston, J.; and Glimcher, M.J.: The Comparative Biochemistry of the Organic Matrix Proteins of Developing Enamel, Arch Oral Biol 12:407-410, 1967.[CrossRef][Medline] [Order article via Infotrieve]
• Katz, E.P.; Seyer, J.; Levine, P.T.; and Glimcher, M.J.: The Comparative Biochemistry of the Organic Matrix of Developing Enamel. II. Ultracentrifugal and Electrophoretic Characterization of Proteins Soluble at Neutral pH, Arch Oral Biol 14:533-539, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Fincham, A.G.; Belcourt, A.B.; Lyaruu, D.M.; and Termine, J.D.: Comparative Protein Biochemistry of Developing Dental Enamel Matrix from Five Mammalian Species, Calcif Tissue Intl 34:182-189, 1982.[CrossRef][Medline] [Order article via Infotrieve]
• Fincham, A.G.; Burkland, G.A.; and Shapiro, I.M.: Lipophilia of Enamel Matrix. A Chemical Investigation of the
• Neutral Lipids and Lipophilic Proteins of Enamel, Calcif Tissue Res 9:247-259, 1971.
• Reisner, A.H.; Nemes, P.; and Bucholtz, C.: The Use of Coomassie Brilliant Blue G250 Perchloric Acid Solution for Staining in Electrophoresis and Isoelectric Focusing on Polyacrylamide Gels, Analyt Biochem 64:509-516, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Keutman, H.T. and Potts, J.T.: Improved Recovery of Methionine after Acid Hydrolysis Using Mercaptoethanol, Analyt Biochem 29:175-185, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Fincham, A.G.; Belcourt, A.B.; Termine, J.D.; Butler, W.T.; and Cothran, W.C.: Dental Enamel Matrix: Sequences of Two Amelogenin Polypeptides, Bioscience Reports 1:771-778, 1981.[CrossRef][Medline] [Order article via Infotrieve]
• Belcourt, A.B.; Fincham, A.G.; and Termine, J.D.: Bovine High Molecular Weight Amelogenin Proteins, Calcif Tissue Intl, In press, 1982.
• Ungewickell, E. and Branton, D.: Assembly Units of Clathrin Coats, Nature 289:420-422, 1981.[CrossRef][Medline] [Order article via Infotrieve]
• Bretscher, A. and Weber, K.: Tropomyosin from Bovine Brain Contains Two Polypeptide Chains of Slightly Different Molecular Weights, FEBS Letts 85:145-148, 1978.[CrossRef][Medline] [Order article via Infotrieve]
• Lyaruu, D.M.; Belcourt, A.B.; Fincham, A.G.; and Termine, J.D.: Neonatal Hamster Molar Tooth Development: Extraction and Characterization of Amelogenins, Enamelins and Soluble Dentin Proteins, Calcif Tissue Intl 34: 86-96, 1982.
• Deutsch, D. and Gedalia, I.: Chemically Distinct Stages in Developing Human Fetal Enamel, Arch Oral Biol 25:635-639, 1980.[CrossRef][Medline] [Order article via Infotrieve]
• Slavkin, H.C.; Mino, W.; and Bringas, P., Jr.: The Biosynthesis and Secretion of Precursor Enamel Protein by Ameloblasts as Visualized by Autoradiography after Tryptophan Administration, Anat Rec 185:289-312, 1976.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 62, No. 1, 11-15 (1983)
DOI: 10.1177/00220345830620010101


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