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Peroxidase Antimicrobial System of Human Saliva: Hypothiocyanite Levels in Resting and Stimulated Saliva

Division of Molecular Biology, University of Alabama in Birmingham, University Station, Birmingham, Alabama 35294

K.M. Pruitt

Division of Molecular Biology, University of Alabama in Birmingham, University Station, Birmingham, Alabama 35294

E.L. Thomas

Department of Biochemistry, St. Jude Children's Research Hospital and The University of Tennessee Center for Health Sciences, Memphis, Tennessee 38101

The antimicrobial oxidizing agent hypothiocyanite ion (OSCN-) was measured in resting (drooling) and stimulated (expectorated) whole saliva. Stimulation of the saliva flow rate resulted in a rapid decrease in OSCN- concentration, whereas the thiocyanate ion (SCN—) concentration and peroxidase activity were increased. The decrease in OSCN — levels was greater than could be accounted for by dilution of the whole saliva volume. Assuming that the antimicrobial activity of the salivary peroxidase system is proportional to OSCN- concentration, this system may be more effective in resting saliva than in stimulated saliva.

REFERENCES

• Hamon, C.B. and Klebanoff, S.J.: A Peroxidase-mediated Streptococcus mitis Dependent Antimicrobial System in Saliva, JExpMed 137:438-450, 1973.[Abstract]
• Hoogendoorn, H.: The Effect of Lactoperoxidase-Thiocyanate-Hydrogen Peroxide on the Metabolism of Cariogenic Microorganisms in vitro and in the Oral Cavity, Mouton, Den Haag, The Netherlands, 1974.
• Tenovuo, J.; Valtakoski, J.; and Knuuttila, M.L.E.: Antibacterial Activity of Lactoperoxidase Absorbed by Human Salivary Sediment and Hydroxyapatite, Caries Res 11:257-262,1977.[Medline] [Order article via Infotrieve]
• Tenovuo, J. and Anttonen, T.: Peroxidase-catalyzed Hypothiocyanite Production in Human Salivary Sediment in Relation to Oral Health, Caries Res 14:269-275, 1980.[Medline] [Order article via Infotrieve]
• Smith, J.W.; Chwang, A.K.; and Thomas, E.L.: Lactoperoxidase Antimicrobial Action in Human Saliva, IADR Progr & Abst 60: No. 509, 1981.
• Tenovuo, J.; Mansson-Rahemtulla, B.; Pruitt, K.M.; and Arnold, R.: Inhibition of Dental Plaque Acid Production by the Salivary Lactoperoxidase Antimicrobial System, Infect Immun 34:208-214, 1981.[Abstract/Free Full Text]
• Germaine, G.R. and Tellefson, L.M.: Effect of Human Saliva on Glucose Uptake by Streptococcus mutans and Other Oral Microorganisms, Infect Immun 31:598-607, 1981.[Abstract/Free Full Text]
• Rotgans, J. and Hoogendoorn, H.: The Effect of Brushing with a Toothpaste Containing Amyloglucosidase and Glucose Oxidase on Dental Caries in Rats, Caries Res 13:150-153,1979.[Medline] [Order article via Infotrieve]
• Koch, G. and Strand, G.: Effect of an Enzyme Dentifrice on Caries. A Two-year Clinical Pilot Study, Swed Dent J 3: 9-13,1979.[Medline] [Order article via Infotrieve]
• Muhlemann, H.R.; Schmid, R.; and Firestone, A.R.: Effect on Rat Caries of Endogenous and Exogenous Hydrogen Peroxide, Caries Res 15:46-53, 1981.[Medline] [Order article via Infotrieve]
• Thomas, E.L.; Bates, K.P.; and Jefferson, M.M.: Peroxidase Antimicrobial System of Human Saliva: Requirements for Accumulation of Hypothiocyanite, J Dent Res 60: 780-796,1981.
• Pruitt, K.M.; Tenovuo, J.; Fleming, W.; and Adamson, M.: Limiting Factors for the Generation of Hypothiocyanite Ion, an Antimicrobial Agent, in Human Saliva, Caries Res, In press, 1981.
• Aune, T.M. and Thomas, E.L.: Accumulation of Hypothiocyanite Ion During Peroxidase-catalyzed Oxidation of Thiocyanate Ion, Eur J Biochem 80:209-214, 1977.[CrossRef][Medline] [Order article via Infotrieve]
• Hoogendoorn, H.; Piessens, J.P.; Scholtes, W.; and Stoddard, L.A.: Hypothiocyanite Ion; the Inhibitor Formed by the System Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, Caries Res 11:77-84, 1977.[Medline] [Order article via Infotrieve]
• Thomas, E.L.: Lactoperoxidase-catalyzed Oxidation of Thiocyanate: The Equilibria Between Oxidized Forms of Thiocyanate, Biochemistry 20:3273-3280, 1981.[CrossRef][Medline] [Order article via Infotrieve]
• Hogg, D.M. and Jago, G.R.: The Antimicrobial Action of Lactoperoxidase. The Nature of the Bacterial Inhibitor, BiochemJ 117:779-790, 1970.[Medline] [Order article via Infotrieve]
• Kersten, H.W.; Moorer, W.R.; and Wever, R.: Thiocyanate as a Cofactor in Myeloperoxidase Activity Against Streptococcus mutans, J Dent Res 60:831-837, 1981.[Abstract/Free Full Text]
• Thomas, E.L.; Bates, K.P.; and Jefferson, M.M.: Hypothiocyanite Ion: Detection of the Antimicrobial Agent in Human Saliva, J Dent Res 59:1466-1472, 1980.[Abstract/Free Full Text]
• Ellman, G.L.: Tissue Sulfhydryl Groups, Arch Biochem Biophys 82:70-77,1959.[CrossRef][Medline] [Order article via Infotrieve]
• Pruitt, K.M.; Adamson, M.; and Arnold, R.: Lactoperoxidase Binding to Streptococci, Infect Immun 25:304-309,1979.[Abstract/Free Full Text]
• Shindler, J.S.; Childs, R.E.; and Bardsley, W.G.: Peroxidase of Human Cervical Mucus. Isolation and Characterization,EurJBiochem 65:325-331, 1976.[Medline] [Order article via Infotrieve]
• Azen, E.A.: Salivary Peroxidase Activity and Thiocyanate Concentration in Human Subjects with Genetic Variants of Salivary Peroxidase, Arch Oral Biol 23:801-805, 1978.[CrossRef][Medline] [Order article via Infotrieve]
• Tenovuo, J. and Valtakoski, J.: The Correlation Between Salivary Peroxidase Activity, Salivary Flow Rate, and the Oxidation-reduction Potentials of Human Saliva and Dental Plaque Suspensions, Acta Odont Scand 34:169-176, 1976.[CrossRef][Medline] [Order article via Infotrieve]
• Globerman, D.Y. and Kleinberg, I.: Intra-oral pO2 and Its Relation to Bacterial Accumulation on the Oral Tissues. In: Proceedings, Saliva and Dental Caries, Sp. Supp. Microbiology Abstracts, Kleinberg, I., Ellison, S.A., and Mandel, I.D., Eds., 1979, pp. 275-292.
• Bjorck, L. and Claesson, 0.: Correlation Between Concentration of Hypothiocyanite and Antibacterial Effect of the Lactoperoxidase System Against Escherichia coli, J Dairy Sci 63:919-922, 1980.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 61, No. 8, 982-985 (1982)
DOI: 10.1177/00220345820610081301


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