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Acid-soluble Bovine Fetal Enamelins

Laboratory of Biological Structure, Skeletal Matrix Biochemistry Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205

A.G. Fincham

Laboratory of Biological Structure, Skeletal Matrix Biochemistry Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205

J.D. Termine

Laboratory of Biological Structure, Skeletal Matrix Biochemistry Section, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20205

High molecular weight enamelin proteins were recovered from conventional acetic acid extracts of fetal bovine enamel and were analyzed by gel filtration and ion-exchange chromatography. This procedure is simple and straightforward, and recovers approximately half of the total tissue enamelin content.

REFERENCES

• Eastoe, J.: Enamel Protein Chemistry - Past, Present and Future, J Dent Res 58(B):753-763, 1979.[Abstract/Free Full Text]
• Termine, J.D.; Belcourt, A.B.; Christner, P.J.; Conn, K.M.; and Nylen, M.U.: Properties of Dissociatively Extracted Fetal Tooth Matrix Proteins. I. Principal Molecular Species in Developing Bovine Enamel, J Biol Chem 255:9760-9768, 1980.
• Belcourt, A.B. and Gillmeth, S.: EDTA Soluble Protein of Human Mature Normal Enamel, Calcif Tissue Int 28:227-231, 1979.[CrossRef][Medline] [Order article via Infotrieve]
• Belcourt, A.B.; Fincham, A.G.; and Termine, J.D.: EDTA Insoluble Protein of Adult Human Enamel, Caries Res, In press.
• Eastoe, J.: The Amino Acid Composition of Proteins from the Oral Tissues. II. The Matrix Proteins in Dentin and Enamel from Developing Human Deciduous Teeth, Arch Oral Biol 8: 633-652,1963.[CrossRef]
• Yanagisawa, T. and Nylen, M.U.: The Relationship Between Matrix and Mineral in Rat Enamel - An Electron Microscopy Study, AADR Progr & Abst 59:No. 343, 1980.
• Fincham, A.G.; Belcourt, A.B.; Lyaruu, D.M.; and Termine, J.D.: Comparative Protein Biochemistry of Developing Dental Enamel Matrix From Five Mammalian Species, Calcif Tissue Int, In press.
• Termine, J.D.; Belcourt, A.B.; Conn, K.M.; and Klein-Man, H.D.: Mineral and Collagen Binding Proteins of Fetal Calf Bone, J Biol Chem 256:10403-10408,1981.[Abstract/Free Full Text]
• Belcourt, A.B.; Fincham, A.G.; and Termine, J.D.: Bovine High Molecular Weight Amelogenin Proteins, Calcif Tissue Int, In press.

Journal of Dental Research, Vol. 61, No. 8, 1031-1032 (1982)
DOI: 10.1177/00220345820610080801


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Belcourt, A.B. Articles by Termine, J.D. Search for Related Content PubMed PubMed Citation Articles by Belcourt, A.B. Articles by Termine, J.D. Social Bookmarking                         What's this?