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Salivary Protein Polymorphisms in Caries-free and Caries-active Adults

Department of Oral Biology, University of Washington, H.S. Bldg. 147 SB-22, Seattle, Washington 98195

B.L. Lamberts

Biochemistry Division, Naval Dental Research Institute, Great Lakes, Illinois 60088

W.F. Bruton

Biochemistry Division, Naval Dental Research Institute, Great Lakes, Illinois 60088

Several salivary proteins are known to exhibit genetic polymorphism, and this study was undertaken to determine whether there was an association of caries experience with specific parotid salivary protein phenotypes. Parotid saliva collected from 46 caries-free and 47 caries-active naval recruits was subjected to SDS, anionic, and cationic polyacrylamide slab gel electrophoresis. Distributions of the acidic proline-rich (Pr), parotid acidic (Pa), double band (Db), parotid size variant (Ps), parotid middle band (Pm), and parotid basic (Pb) protein phenotypes were similar in both caries-free and caries-active subjects. Further, overall protein patterns on SDS, anionic, and cationic slab gels did not differ between the two groups.

REFERENCES

• Zengo, A.N.; Mandel, I.D.; Goldman, R.; and Khu-Rama, H.S.: Salivary Studies in Human Caries Resistance, Arch Oral Biol 16:557-560, 1971.[CrossRef][Medline] [Order article via Infotrieve]
• Mandel, I.D.; Zorn, M.; Ruiz, R.; Thompson, R.H.; and Ellison, S.A.: The Proteins and Protein-bound Carbohydrate of Parotid Saliva in Caries-immune and Caries-active Adults, Arch Oral Biol 10:471-475, 1965.[CrossRef][Medline] [Order article via Infotrieve]
• Mandel, I.D. and Bennick, A.: Quantitation of Salivary Acidic Proline-rich Phosphoproteins in Oral Disease, AADR Progr & Abst 59:No. 909, 1980.
• Mandel, I.D. and Zengo, A.N.: Genetic and Chemical Aspects in Caries Resistance. In: Comparative Immunology of the Oral Cavity, Washington, D.C.: U.S. Government Printing Office, 1973, pp. 118-137.
• Cowman, R.A.; Schaefer, S.J.; Fitzgerald, R.J.; Rosner, D.; Shklair, I.L.; and Walter, R.G.: Differential Utilization of Proteins in Saliva from Caries-active and Caries-free Subjects as Growth Substrates by Plaque-forming Streptococci, J Dent Res 58:2019-2027, 1979.[Abstract/Free Full Text]
• Azen, E.A.: Genetic Polymorphisms in Human Saliva: An Interpretive Review, Biochem Genet 16:79-99, 1978.[CrossRef][Medline] [Order article via Infotrieve]
• Arneberg, P.: Partial Characterization of Five Glycoprotein Fractions Secreted by Human Parotid Glands, Arch Oral Biol 19:921-928,1974.[CrossRef][Medline] [Order article via Infotrieve]
• Laemmli, U.K.: Cleavage of Structural Proteins During Assembly of the Head of Bacteriophage T4, Nature 227: 680-685, 1970.[CrossRef][Medline] [Order article via Infotrieve]
• Weber, K. and Osborn, M.: Proteins and Sodium Dodecyl Sulfate: Molecular Weight on Polyacrylamide Gels and Related Procedures. In: The Proteins, Vol. 1, Neurath, H. and Hill, R.L., Eds., New York, NY: Academic Press, 1975, pp. 179-223.
• Smith, Q.T.; Runchey, C.R.; and Shapiro, B.L.: Polyacrylamide Gel Slab Electrophoresis of Human Salivary Proteins, Arch Oral Biol 19:407-410, 1974.[CrossRef][Medline] [Order article via Infotrieve]
• Jordan, E.M. and Raymond, S.: Gel Electrophoresis: A New Catalyst for Acid Systems, Anal Biochem 27:205-211, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Steck, G.; Leithard, P.; and Burk, R.R.: Detection of Basic Proteins and Low Molecular Weight Peptides in Polyacrylamide Gel by Formaldehyde Fixation, Ann Biochem 107: 21-24, 1980.[CrossRef]
• Friedman, R.D.; Merritt, A.D.; and Rivas, M.L.: Genetic Studies of Human Acidic Salivary Protein (Pa), Am J Hum Genet 27:292-303, 1975.[Medline] [Order article via Infotrieve]
• Azen, E.A.: Genetic Polymorphism of the Basic Proteins from Parotid Saliva, Science 176:673-674, 1972.[Abstract/Free Full Text]
• Azen, E.A. and Denniston, C.: Polymorphism of Ps (Parotid Size Variant) and Detection of a Protein (PmS) Related to the Pm (Parotid Middle Band) System with Genetic Linkage of Ps and Pm to Gl, Db, and Pr Genetic Determinants, Biochem Genet 18:483-502, 1980.
• Bennick, A.; Wong, R.; and Cannon, M.: Structure and Biological Activities of Salivary Acidic Proline-rich Phosphoproteins. In: Calcium Binding Proteins and Calcium Function, Wasserman, R.H.; Corradino, R.A.; Carafoli, E.; Krisinger, R.H.; MacLennan, D.H.; and Steigel, F.L., Eds., New York, NY: Elsevier North-Holland, Inc., 1977, pp. 391-400.
• Horowitz, S.L.; Osborne, R.H.; and De George, F.V.: Caries Experience in Twins, Science 128:300-301, 1958.[Free Full Text]
• Caldwell, R.C. and Finn, S.B.: Comparisons of the Caries Experience Between Identical and Fraternal Twins and Unrelated Children, J Dent Res 39:693-694, 1960.
• Finn, S.B. and Caldwell, R.C.: Dental Caries in Twins. I. A Comparison of the Caries Experience of Monozygotic and Dizygotic Twins and Unrelated Children, Arch Oral Biol 8: 571-585,1963.[CrossRef]
• Bordoni, N.; Dono, R.; Manfredi, C.; and Alle-Grotti, I.: Prevalence of Dental Caries in Twins, J Dent Child 40:440-443, 1973.[Medline] [Order article via Infotrieve]
• Fairpo, C.G.: Total Caries Experience in Monozygotic and Like-sexed Dizygotic Twins of Caucasoid Origin Aged 5 to 15 Years, Arch Oral Biol 24:491-494, 1979.[CrossRef][Medline] [Order article via Infotrieve]
• Smith, Q.T.; Shapiro, B.L.; and Hamilton, M.J.: Polyacrylamide Gel Electrophoresis of Twin and Non-twin Parotid Salivary Proteins, Proc Soc Exp Biol Med 151:535-538, 1976.[CrossRef][Medline] [Order article via Infotrieve]
• Woolf, B.: On Estimating the Relation Between Blood Group and Disease, Ann Hum Genet 19:251-253, 1955.[Medline] [Order article via Infotrieve]
• Kaufmann, D.L. and Keller, P.J.: The Basic Proline-rich Proteins in Human Parotid Saliva from a Single Subject, Arch Oral Biol 24:249-256, 1979.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 61, No. 2, 393-396 (1982)
DOI: 10.1177/00220345820610020501


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