This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Fukushima, K. Articles by Ikeda, T. Search for Related Content PubMed PubMed Citation Articles by Fukushima, K. Articles by Ikeda, T. Social Bookmarking                         What's this?

Effects of Exogenous Insoluble Glucan Primer on Insoluble Glucan Synthesis by Streptococcus mutans

Department of Bacteriology, Nihon University School of Dentistry at Matsudo, Matsudo 271, Japan

R. Motoda

Department of Bacteriology, Nihon University School of Dentistry at Matsudo, Matsudo 271, Japan

T. Ikeda

Department of Bacteriology, Nihon University School of Dentistry at Matsudo, Matsudo 271, Japan

The synthesis of water-insoluble glucan by a glucosyltransferase preparation from Streptococcus mutans is associated with a lag period lasting several minutes. The addition of small amounts of glucan containing significant amounts of -(13) linkages causes a marked reduction of the lag 'period with little change in the reaction rate. A similar effect was not observed with other glucans, such as dextran, dextrin, and cellulose.

REFERENCES

• Gibbons, R.J. and Van Houte, J.: Bacterial Adherence in Oral Microbial Ecology, Ann Rev Microbiol 29:19-44, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Gibbons, R.J. and Nygaard, M.: Synthesis of Insoluble Glucan and Its Significance in the Formation of Gelatinous Deposits by Plaque-forming Streptococci, Arch Oral Biol 13:1249-1262, 1968.[CrossRef][Medline] [Order article via Infotrieve]
• Mukasa, H. and Slade, H.D.: Mechanism of Adherence of Streptococcus mutans to Smooth Surfaces. I. Roles of Insoluble Dextran-levan Synthetase Enzymes and Cell Wall Polysaccharide Antigens in Plaque Formation, Infect Immun 8:555-562, 1973.[Abstract/Free Full Text]
• Scherp, H.W.: Dental Caries, Prospects for Prevention, Science 173:1199-1205, 1971.[Abstract/Free Full Text]
• Ciardi, J.E.; Beaman, A.J.; and Witten-Berger, C.L.: Purification, Resolution, and Interaction of the Glucosyltransferases of Streptococcus mutans 6715, Infect Immun 18:237-246,1977.[Abstract/Free Full Text]
• Fukui, K.; Fukui, Y.; and Moriyama, T.: Purification and Properties of Dextransucrase and Invertase from Streptococcus mutans, J Bacteriol 118:796-804, 1974.[Abstract/Free Full Text]
• Mukasa, H. and Slade, H.D.: Mechanism of Adherence of Streptococcus mutans to Smooth Surfaces. III. Purification and Properties of the'Enzyme Complex Responsible for Adherence, Infect Immun 10:1135-1145, 1974.[Abstract/Free Full Text]
• Kuramitsu, H.K.: Characterization of Extracellular Glucosyltransferase Activity of Streptococcus mutans, Infect Immun 12: 738-749, 1975.[Abstract/Free Full Text]
• Scales, W.R.;Long, L.W.; and Edwards, J.R.: Purification and Characterization of a Glucosyltransferase Complex from the Culture Broth of Streptococcus mutans FA-1, Carbo Res 42:325-338, 1975.[CrossRef][Medline] [Order article via Infotrieve]
• Gibbons, R.J. and Keyes, P.H.: Inhibition of Insoluble Dextran Synthesis, Plaque Formation, and Dental Caries in Hamsters by Low Molecular Weight Dextran, Arch Oral Biol 14:721-724, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Johnson, M.C.; Bozzola, J.J.; Schec-Meister, I.L.; and Shklair, I.L.: Biochemical Study of the Relationship of Extracellular Glucan to Adherence and Cariogenicity in Streptococcus mutans and an Extracellular Polysaccharide Mutant, J Bacteriol 129:351-357, 1977.[Abstract/Free Full Text]
• Germaine, G.R.; Harlander, S.K.; Leung, W.S.; and Schachtele, C.F.: Streptococcus mutans Dextransucrase: Functioning of Primer Dextran and Endogenous Dextranase in Water-soluble and Water-insoluble Glucan Synthesis, Infect Immun 16:637-648,1977.[Abstract/Free Full Text]
• McCabe, M.M. and Smith, E.E.: Origin of Cell-associated Dextransucrase of Streptococcus mutans, Infect Immun 7:829-838, 1973.[Abstract/Free Full Text]
• Mukasa, H.; Shimamura, A.; and Tsu-Mori, H.: Effect of Salts on Water-insoluble Glucan Formation by Glucosyltransferase of Streptococcus mutans, Infect Immun 23:564-570, 1979.[Abstract/Free Full Text]
• Ikeda, T.; Shiota, T.; McGhee, J.R.; Otake, S.; Michalek, M.; Ochiai, K.; Hirasawa, M.; and Sugimoto, K.: Virulence of Streptococcus mutans: Comparison of the Effects of a Coupling Sugar and Sucrose on Certain Metabolic Activities and Cariogenicity, Infect Immun 19:477-480, 1978.[Abstract/Free Full Text]
• Ebisu, S.; Misaki, A.; Kato, K.; and Kotani, S.: The Structure of Water-insoluble Glucan of Cariogenic Streptococcus mutans, Formed in the Absence and Presence of Dextranase, Carbo Res 38:374-381, 1974.
• Dubois, M.; Gilles, A.; Hamilton, J.K.; Robers, P.A.; and Smith, F.: Colorimetric Method for Determination of Sugars and Related Substances, Anal Chem 28:350-356,1956.
• Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275,1951.[Free Full Text]
• Donkersloot, J.A.; Flatow, U.; Gib-Son, E.; and Chassy, B.M.: Characterization of Glucosyltransferase-deficient Plasmid-containing Mutants of Streptococcus mutans LM-7, Infect Immun 21:320-327, 1978.[Abstract/Free Full Text]
• Germaine, G.R.; Chludzinski, A.M.; and Schachtele, C.F.: Streptococcus mutans Dextransucrase: Requirement for Primer Dextran, J Bacteriol 120:287-294, 1974.[Abstract/Free Full Text]
• Chludzinski, A.M.; Germaine, G.R.; and Schachtele, C.F.: Streptococcus mutans Dextransucrase: Purification, Properties, and Requirement for Primer Dextran, J Dent Res 55(C):75-86, 1976.
• Montville, T.J.; Cooney, C.L.; and Sinskey, A.J.: Measurement and Synthesis of Insoluble and Soluble Dextran by Streptococcus mutans, J Dent Res 56:983-989, 1977.[Abstract/Free Full Text]
• Fukushima, K.; Motoda, R.: Takada, K.; and Ikeda, T.: Effects of Various Polysaccharides on Insoluble Glucan Synthesis by S. mutans, IADR Progr & Abst 59:No. 960,1980.

Journal of Dental Research, Vol. 60, No. 9, 1707-1712 (1981)
DOI: 10.1177/00220345810600091201


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C.D. Wu-Yuan, C.Y. Chen, and R.T. Wu Gallotannins Inhibit Growth, Water-insoluble Glucan Synthesis, and Aggregation of Mutans Streptococci Journal of Dental Research, January 1, 1988; 67(1): 51 - 55. [Abstract] [PDF]

				K. Takada and K. Fukushima Effects of Certain Salts on Glucosyltransferase Synthesis by Streptococcus mutans Strain PS-14 Journal of Dental Research, March 1, 1986; 65(3): 452 - 455. [Abstract] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Fukushima, K. Articles by Ikeda, T. Search for Related Content PubMed PubMed Citation Articles by Fukushima, K. Articles by Ikeda, T. Social Bookmarking                         What's this?