This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by DeRenzis, F. A. Search for Related Content PubMed PubMed Citation Articles by DeRenzis, F. A. Social Bookmarking                         What's this?

Endotoxin-inactivating Potency of Hydrogen Peroxide: Effect on Cell Growth

Department of Periodontology, Temple University School of Dentistry, Philadelphia, Pennsylvania 19140

An in vitro system was used to study the detoxifying potential of H₂O₂ on bacterial endotoxin. L929 fibroblasts were exposed to H202-treated and -untreated endotoxin. Growth and viability assays were made at 24, 48, 72, and 96 h. The cultures exposed to H202-treated endotoxin showed a normal growth rate, whereas the cultures exposed to untreated endotoxin displayed a substantial reduction in growth. Implications are presented as to the potential role of H202 in the pathogenesis and treatment of periodontal disease.

REFERENCES

• Campbell, D.H. and Cherkin, A.: The Destruction of Pyrogens by Hydrogen Peroxide, Science 102:535-537, 1945.[Free Full Text]
• Niukian, K.; Eichel, B.; and Shahrik, H.A.: Histochemical Disclosure of Endogenous and H202-dependent Reducing Enzyme Activities in Leukocytes and Other Cytological Entities from the Human Oral Cavity, Arch Oral Biol 18:505-515, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Holmberg, K. and Hallander, H.O.: Production of Bactericidal Concentration of Hydrogen Peroxide by Streptococcus sanguis, Arch Oral Biol 18:423433, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Mergenhagen, S.E.; Hampp, E.G.; and Scherp, H.W.: Preparation and Biological Activities of Endotoxins from Oral Bacteria, J Infect Dis 108:304-310, 1961.[Medline] [Order article via Infotrieve]
• Simon, B.; Goldman, H.; Reuben, M.; and Baker, E.A.: A Reproducible Quantitative Method for Determining the Amount of Endotoxin in Human Gingival Exudate, J Periodontol 40:695-701, 1969.[Medline] [Order article via Infotrieve]
• SCHWARTZ, J.; STINSON, F.L.; and PAR-KER, R.B.: The Passage of Tritiated Bacterial Endotoxin Across Intact Gingival Crevicular Epithelium, J Periodontol 43:270-276, 1972. 7. ALEO, J.J.; DeRENZIS, F.A.; FARBER, P.; and VARBONCOEUR, A.: The Presence and Biologic Activity of Cementum-bound Endotoxin, J Periodontol 45:672-675, 1974. 8. BERGMAN, S. and NILSSON, S.B.: Effect of Endotoxin on Embryonal Chick Fibroblasts Cultured in Monolayer, Acta Pathol Micro Biol Scand 59:161-164, 1963.
• Singer, R.E. and Dutton, W.G.: A Comparison of the Effects of Endotoxin Upon Fibroblast Proliferation and Macromolecular Synthesis, J Dent Res 58:1634-1639,1979.[Abstract/Free Full Text]
• Tzamouranis, A.; Matthys, J.; Ishi-Kawa, I.; and Cimasoni, G.: Increase of Endotoxin Concentration in Gingival Washings During Experimental Gingivitis in Man, JPeriodontol 50:175-177, 1979.[Medline] [Order article via Infotrieve]
• Shapiro, L.; Lodato, F.M.; Courant, P.R.; and Stallard, R.E.: Endotoxin Determinations in Gingival Inflammation, J Periodontol 43:591-596, 1972.[Medline] [Order article via Infotrieve]
• Taichman, N.S. and Courant, P.: The Production of Hemorrhagic Necrosis in the Hamster Cheek Pouch by Bacterial Endotoxins and Catecholamines, Arch Oral Biol 10:541-543,1965.[CrossRef][Medline] [Order article via Infotrieve]
• Hausmann, E.; Weinfield, N.; and Miller, W.A.: Effects of Lipopolysaccharides on Bone Resorption in Tissue Culture, Calcif Tiss Res 9:272-282, 1972.[CrossRef]
• Aleo, J.J.; DeRenzis, F.A.; and Farber, P.A.: In vitro Attachment of Human Gingival Fibroblasts to Root Surfaces, J Periodontol 46:639-645,1975.[Medline] [Order article via Infotrieve]
• Clem, H.W. and Klebanoff, J.S.: Inhibitory Effect of Saliva on Glutamic Acid Accumulation by Lactobacillus acidophilus and the Role of the Lactoperoxidase-thiocyanate System, J Bact 91:1848-1853, 1966.[Abstract/Free Full Text]
• Iyer, G.Y.N.; Islam, F.M.; and Quastel, M.J.: Biochemical Aspects of Phagocytosis, Nature 192:535-541, 1961.[CrossRef]
• Paul, B. and Sbarra, S.A.: The Role of the Phagocyte in Host-parasite Interactions. The Direct Quantitative Estimation of H202 in Phagocytizing Cells, Biochem Biophys Acta 156:168-178, 1968.[Medline] [Order article via Infotrieve]
• Holmes, B.; Page, R.A.; and Good, A.R.: Studies of the Metabolic Activity of Leukocytes from Patients with Genetic Abnormality of Phagocytic Function, J Clin Invest 46:1422-1428, 1967.[Medline] [Order article via Infotrieve]
• Mandell, G.L. and Hook, E.W.: Leukocyte Bactericidal Activity in Chronic Granulomatous Disease: Correlation of Bacterial Hydrogen Peroxide Production and Susceptibility to Intracellular Killing, J Bact 100:531-535,1969.[Abstract/Free Full Text]
• Cianciola, L.J.; Genco, R.J.; Patters, M.R.; McKenna, J.; and Van Oss, C.J.: Defective Polymorphonuclear Leukocyte-function in a Human Periodontal Disease, Nature 265:445-447, 1977.[CrossRef][Medline] [Order article via Infotrieve]
• DeRenzis, F.A.: Endotoxin-inactivating Potency of Molecular Oxygen: Effect on Cell Growth, J Dent Res, 59:1521, 1980.[Free Full Text]

Journal of Dental Research, Vol. 60, No. 5, 933-935 (1981)
DOI: 10.1177/00220345810600051401


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by DeRenzis, F. A. Search for Related Content PubMed PubMed Citation Articles by DeRenzis, F. A. Social Bookmarking                         What's this?