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Identification of Hyaluronidase Activity in Rabbit Dental Pulp

Department of Endodontology and Periodontology

T. Nakajima

Department of Endodontology and Periodontology

K. Ikunaga

Department of Endodontology and Periodontology

H. Shidahara

Department of Endodontology and Periodontology

H. Okamoto

Department of Endodontology and Periodontology

K. Okuda

Department of Biochemistry, Hiroshima University School of Dentistry, 1-2-3 Kasumi, Minami-ku, Hiroshima 734, Japan

A hyaluronidase activity was demonstrated in rabbit dental pulp. The optimum pH of the enzyme was 3.8. The enzyme activity was enhanced by protamine and poly-L-lysine and was inhibited by iodoacetamide, ferric ion, and ferrous ion in decreasing order. The product of the enzyme reaction was identified as tetrasaccharide. From these results it was concluded that the enzyme exists in pulp tissue and is functioning for degradation of proteoglycans in situ.

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Journal of Dental Research, Vol. 60, No. 4, 850-854 (1981)
DOI: 10.1177/00220345810600041601


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