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Role of Organic Phosphate in Mineralization of Bone in vitro

Medical Research Council Group in Periodontal Physiology, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada

Calvarial periostea were dissected from 17-day-old embryonic chicks and folded with the osteogenic cells in apposition. The folded explants were cultured for up to six d on serum and plasma clots or in serum-free hormone-supplemented completely-defined medium. Osteoid consistently formed in such cultures in both types of media, and this osteoid mineralized when appropriate levels of β-glycerophosphate were added to each type of medium. The data presented suggest that the levels of organic phosphate might be more important than inorganic phosphates as a limiting factor in the initiation of mineralization of bone in vitro.

REFERENCES

• Nikiforuk, G. and Fraser, G.: Etiology of Enamel Hypoplasia and Intetglobular Dentine. Metab Bone Dis and Rel Res 2:17-23, 1979.[CrossRef]
• Shafer, W.G.; Hine, M.K.; and Levy, B.M.: A Textbook of Oral Pathology, 3rd ed., Toronto: W.B. Saunders Co., 1974.
• Harris, W.H.,; Heaney, R.P.; Davis, L.A.; Weinberg, E.H.; Coutts, R.D.; and Schiller, A.L.; Stimulation of Bone Formation in vivo by Phosphate Supplementation, Calc Tiss Res 22:85-98, 1978.
• Binderman, I.; Greene, R.M.; and Pennypacker, J.P.: Calcification of Differentiating Skeletal Mesencliytne in vitro, Science 206:222-224, 1979.[Abstract/Free Full Text]
• Tenenbaum, H.C. and Heersche, J.N.M.: Differentiation of Osteoblasts and Formation of Mineralized Bone in vitro, Calc Tissite Int, In press, 1981.
• Nijweide, P.J. and Van der Plas, A.: Calcium and Strontium Metabolism of Bone, Proc Kon Ned Akad Wet C78:410-417, 1975. 7. Paul, J.: Cell and Tissue Culture, Churchill and Livingston, 1975.
• Gaillard, P.J.: Developmental Changes in the Composition of Body Fluids in Relation to the Growth and Differentiation of Tissue Cultures, Protoplasma XXIII: 145-174. 1934.
• Tenenbaum, H.; Cheng, P.; Pritzker, K.; and Heersche, J.N.M.: Manuscript in preparation.
• Albritton, E.C.: Standard Values in Blood, Philadelphia, PA: W.B. Saunders Co., 1952.
• Raisz, L.G.; Dietrich, J.W.; and Cana-Lis, E.M.: Factors Influencing Bone Formation in Organ Cultures, Isr J Med Sci 12:108-114,1976.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 60, No. 3 Suppl, 1586-1589 (1981)
DOI: 10.1177/002203458106000308011


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Tenenbaum, H.C. Search for Related Content PubMed PubMed Citation Articles by Tenenbaum, H.C. Social Bookmarking                         What's this?