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Clinical ScienceLactoferrin Concentration in Human Parotid Saliva as Measured by an Enzyme-linked Immunosorbent Assay (ELISA)
Camille Dipaola
Division of Preventive Dentistry, School of Dental and Oral Surgery, Columbia University, 630 West 168 Street, New York, New York 10032
Irwin D. Mandel
Division of Preventive Dentistry, School of Dental and Oral Surgery, Columbia University, 630 West 168 Street, New York, New York 10032
The enzyme-linked immunosorbent assay (ELISA) was found to be a reliable and accurate method for quantitating lactoferrin in parotid fluid. In saliva from normal glands, the concentration was usually below 1 mg% and was always higher in unstimulated than in stimulated secretion.
REFERENCES
- Masson, P.; Herremens, J.F.; and Dive, C.: An Iron Binding Protein Common to Many External Secretions, Clin Chim Acta 14:735-739,1966.[CrossRef]
- Cole, M.F.; Arnold, R.R.; Mestecky, J.; Prince, S.; Kulhavy, R.; and McGhee, J.R.: Studies with Human Lactoferrin and S. mutans, in Microbial Aspects of Dental Caries. H. M. Stiles, W. J. Loesche and T. C. O'Brien, Eds., Washington: Information Retrieval, 1976, pp. 359-373.
- Tabak, L.; Mandel, I.D.; Karlan, D.; and Baurmash, H.: Alterations in Lactoferrin in Salivary Gland Disease, JDent Res 57: 43-47,1978.
- Engvall, E. and Perlmann, P.: Enzyme Linked Immunosorbent Assay (ELISA) Quantitative Assay of Immunoglobulin G, Immunochem 8:871-874,1971.[CrossRef][Medline]
[Order article via Infotrieve]
- Voller, A.; Bartlett, A.; and Bidwell, D.E.: Enzyme Immunoassays with Special Reference to ELISA Technique, Jour of Clin Path 31:507-520,1978.[CrossRef]
- Mandel, I.D. and Baurmash, H.: Immunoglobulins in Diseases of the Salivary Glands, in Secretory Immunity and Infection. McGhee, J.R., Mestecky, J., and Babb, J.L., Eds., New York: Plenum Press, 1978, pp. 839-848.
- Avrameas, S.: Coupling of Enzymes to Proteins with Gluteraldehyde, Immunochem 6:43-52,1969.[CrossRef][Medline]
[Order article via Infotrieve]
- Brandtzaeg, P.: Human Secretory Immunoglobulins - Concentrations of Parotid IgA and Other Secretory Proteins in Relation to the Rate of Flow and Duration of Secretory Stimulus, Arch Oral Biol 16:1295-1310, 1976. *[CrossRef]
- Arnold, R.R.; Pruitt, K.M.; Cole, M.F.; Adamson, J.M.; and McGhee, J.R.: Salivary Antibacterial Mechanisms in Immunodeficiency, Proceedings Saliva and Dental Caries, Kleinberg, I., Ellison, S.A., Mandel, I.D., Eds., Sp. Supp. Microbiology Abstracts, 1979, pp. 449-462.
Journal of Dental Research, Vol. 59, No. 9,
1463-1465 (1980)
DOI: 10.1177/00220345800590090101
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