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Purification and Characterization of Galactosyltransferase in Human Parotid Saliva

Department of Preventive Dentistry, Tokushima University School of Dentistry, Kuramotocho, Tokushima City 770, Japan

Tateshi Taniguchi

Department of Preventive Dentistry, Osaka University Dental School, Kitaku, Osaka 530, Japan

Hideki Nonaka

Department of Preventive Dentistry, Tokushima University School of Dentistry, Kuramotocho, Tokushima City 770, Japan

Satoshi Shizukuishi

Department of Preventive Dentistry, Osaka University Dental School, Kitaku, Osaka 530, Japan

Akira Tsunemitsu

Department of Preventive Dentistry, Osaka University Dental School, Kitaku, Osaka 530, Japan

The galactosyltransferase has been purified from human parotid saliva by ammonium sulfate precipitation (25-70% saturation), followed by repeated affinity chromatography on Sepharose--lactalbumin. The molecular weight of the enzyme was estimated to be approximately 56,000. The enzyme catalyzes the transfer of galactose from UDP-galactose to the exposed N-acetylglucosamine residues derived from glycoproteins, forming a Galβ(1-4)GlcNAc linkage.

REFERENCES

• Spiro, M.J. and Spiro, R.G.: Glycoprotein Biosynthesis: Studies on Thyroglobulin, Thyroid Galactosyltransferase, J Biol Chem 243:6529-6537,1968.[Abstract/Free Full Text]
• Schachter, H.; Mcguire, E.J.; and Roseman, S.: Sialic Acid. III. A Uridine Diphosphate D-Galactose: Mucin Galactosyltransferase from Porcine Submaxillary Gland, J Biol Chem 246:5321-5328, 1971.[Abstract/Free Full Text]
• Jato-Rodriguez, J.J. and Mookerjea, S.: UDP-Galactose: Glycoprotein Galactosyltransferase Activity in Tissue from Developing Rat, Proc Canad Fed Biol Sci 16:14, 1973.
• Letts, P.T.; Meistrich, M.L.; Bruce, W.R.; and Schachter, H.: Glycoprotein Glycosyltransferase Levels During Spermatogenesis in Mice, Biochem Biophys Acta 343: 192-207, 1974.[Medline] [Order article via Infotrieve]
• Bauer, C.H.; Hassels, B.F.; and Reut-Ter, W.G.: Galactose Metabolism in Regenerating Rat Liver, Biochem J 154:141-147,1976.[Medline] [Order article via Infotrieve]
• Babad, H. and Hassid, W.Z.: Soluble Uridine Diphosphate D-Galactose β-4-D-Galactosyltransferase from Bovine Milk, J Biol Chem 241:2672-2678, 1966.[Abstract/Free Full Text]
• Nelson, J.D.; Jato-Rodriguez, J.J.; and Mookerjea, S.: Occurrence of Soluble Glycosyltransferase in Human Amniotic Fluid, Biochem Biophys Res Commun 55:530-537, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Bosman, H.B.: Bacterial Glycoprotein: Identification and Properties of Glycoprotein Glycosyltransferase in Escherichia coli, Biochem Biophys Acta 252:369-387, 1971.[Medline] [Order article via Infotrieve]
• Forsthoefel, A. and Mishra, N.C.: Galactosyltransferase of Neurospora, Experimentia 33:846-865, 1977.
• Recommendations of the International Union of Pure and Applied Chemistry and the International Union of Biochemistry, Enzyme Nomenclature, Elsevier, Amsterdam, 1972.
• Nakamura, R.; Watanabe, T.; Yasu-Take, A.; and Tsunemitsu, A.: The Presence of Uridine-5'-Diphospho-Galactose: N-Acetylglucosamine Galactosyltransferase in Human Saliva, J Dent Res 53:937, 1974.[Free Full Text]
• Nakamura, R.; Watanabe, T.; and Tsunemitsu, A.: Some Properties of Uridine 5'-Diphosphogalactose: N-Acetylglucosamine Galactosyltransferase in Human Parotid Saliva, J Dent Res 54:808-813, 1975.[Abstract/Free Full Text]
• Keene, H.J.: Parotid Fluid Collection System with Vacuum-trap Modification, J Dent Res 42:1041, 1963.[Free Full Text]
• Desalegui, M. and Plonska, H.: Preparation and Properties of Porcine Submaxillary Mucins, Archs Biochem Biophys 129: 49-56, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Friedman, R.D.; Merritt, A.D.; and Bixler, D.: Immunological and Chemical Comparison of Heterogenous Basic Glycoprotein in Human Parotid Saliva, Biochem Biophys Acta 230:599-602, 1971.[Medline] [Order article via Infotrieve]
• Payza, N.; Rizui, S.; and Pigman, W.: Studies of Action of Acids and Bases on Porcine Submaxillary Mucin, Archs Biochem Biophys 129:69-74, 1969.
• Spiro, R.G.: Periodate Oxidation of the Glycoprotein Fetuin, J Biol Chem 239:567-573,1964.[Free Full Text]
• Li, Y.-H.; Li, S.-C.; and Shetlar, M.R.: Isolation of Glycopeptides from Rat Liver Microsomes Involved in the Biosynthesis of Plasma Glycoprotein, J Biol Chem 243: 656-665, 1968.[Abstract/Free Full Text]
• Trayer, L.P. and Hill, R.L.: The Purification and Properties of the A Protein of Lactose Synthetase, J Biol Chem 246:6666-6675, 1971.[Abstract/Free Full Text]
• Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F.: Colorimetric Method for the Determination of Sugars and Related Substances, Analyt Chem 28:350-356, 1956.
• Hartree, E.F.: Determination of Protein: A Modification of the Lowry Method that Gives a Linear Photometric Response, Analyt Biochem 48:422-427, 1972.[CrossRef][Medline] [Order article via Infotrieve]
• Hakomori, S.: A Rapid Permethylation of Glycolipid and Polysaccharide Catalyzed by Methylsulfinyl Carbanion in Dimethyl Sulfoxide, J Biochem 55:205-208, 1964.[Free Full Text]
• Standford, P.A. and Conrad, H.E.: The Structure of the Aerobacter aerogenes A3(S1) Polysaccharide. I. A Re-examination Using Improved Procedures for Methylation Analysis, Biochemistry 5:1508-1517, 1966.[CrossRef][Medline] [Order article via Infotrieve]
• Bjorndal, H.; Lindberg, B.; and Svensson, S.: Gas-liquid Chromatography of Partially Methylated Alditols as their Acetates, Acta Chem Scand 21:1801-1804, 1967.[CrossRef]
• Weber, K. and Osborn, M.: The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-polyacrylamide Gel Electrophoresis, J Biol Chem 244:4406-4412, 1969.[Abstract/Free Full Text]
• Andrews, P.: The Gel Filtration Behavior of Proteins Related to their Molecular Weights over a Wide Range, Biochem J 96:595-606, 1965.[Medline] [Order article via Infotrieve]
• Vesterberg, O. and Svensson, H.: Isoelectric Fractionation, Analysis, and Characterization of Ampholites in Natural pH Gradients. IV. Further Studies on the Resolving Power in Connection with Separation of Myoglobins, Acta Chem Scand 20:820-834, 1966.[Medline] [Order article via Infotrieve]
• Stellner, K.; Saito, H.; and Hakomori, S.: Determination of Aminosugar Linkages in Glycolipids by Methylation: Aminosugar Linkages of Ceramide Pentasaccharides of Rabbit Erythrocytes and of Forssman Antigen, Archs Biochem Biophys 155:464-472, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Barker, R.; Olsen, K.W.; Shaper, J.H.; and Hill, R.L.: Agarose derivatives of Uridine Diphosphate and N-Acetylglucosamine for the Purification of a Galactosyltransferase, J Biol Chem 247:7135-7147, 1972.[Abstract/Free Full Text]
• Nakamura, R.; Iwasaki, E.; Shibata, S.; Matsumura, T.; Shizukuishi, S.; and Tsunemitsu, A.: Lactose Synthase Activity of Galactosyltransferase from Human Parotid Saliva, J Dent Res 56:181-184, 1977.[Abstract/Free Full Text]
• Rao, A.K.; Garver, F.; and Mendicino, J.: Biosynthesis of the Carbohydrate Units of Immunoglobulins. I. Purification and Properties of Galactosyltransferase from Swine Mesentary Lymph Nodes, Biochemistry 15: 5001-5009, 1976.[CrossRef][Medline] [Order article via Infotrieve]
• Magee, S.C.; Mawal, R.; and Ebner, K.E.: Proteolytic Conversion of the Molecular Forms of Bovine Milk Galactosyltransferase, J Biol Chem 248:7565-7569, 1973.[Abstract/Free Full Text]
• Prieels, J.P.; Maes, E.; Dolmans, M.; and Leonis, J.: Heterogeneity of Human Milk β(1-4)-D-Galactosyltransferase, Eur J Biochem 60:525-531,1975.[Medline] [Order article via Infotrieve]
• Watkins, W.M. and Hassid, W.Z.: Enzymatic Synthesis of Lactose from Uridine Diphosphate D-Galactose and D-Glucose, Biochem Biophys Res Commun 5:260-264, 1961.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 59, No. 8, 1374-1381 (1980)
DOI: 10.1177/00220345800590080301


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Nakamura, R. Articles by Tsunemitsu, A. Search for Related Content PubMed PubMed Citation Articles by Nakamura, R. Articles by Tsunemitsu, A. Social Bookmarking                         What's this?