This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kadis, B. Articles by Seibert, S. Search for Related Content PubMed PubMed Citation Articles by Kadis, B. Articles by Seibert, S. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL Social Bookmarking                         What's this?

Characterization of Osteoblast-like Cells from Fetal Rat Calvaria

Southern Illinois University, School of Dental Medicine, Edwardsville, Illinois 62026

J.M. Goodson

Forsyth Dental Center, Boston, Massachusetts 02115

S. Offenbacher

Harvard School of Dental Medicine, Boston, Massachusetts 02115

J.W. Bruns

University of California San Francisco, San Francisco, California 94143

S. Seibert

Southern Illinois University, School of Dental Medicine, Edwardsville, Illinois 62026

An improved method is described for the isolation of osteoblast-like cells from fetal rat calvaria. The cells are mononuclear, 8.3µ in diameter, alkaline phosphate positive, and possess rough endoplasmic reticulum, mitochondria, Golgi, and cytoplasmic processes. Isolated bone cells synthesize collagen and cholesterol, grow to confluency in primary culture, and respond to hormonal stimulation.

REFERENCES

• Peck, W.A.; Birge, S.J.; and Fedak, S.A.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation, Science 146:1476-1477,1964.[Abstract/Free Full Text]
• Yu, J.H.; Wells, H.; Ryan, W.J.; and Lloyd, W.S.: Effects of Prostaglandins and Other Drugs on the Cyclic AMP Content of Cultured Bone Cells, Prostaglandins 12: 501-513, 1976.[CrossRef][Medline] [Order article via Infotrieve]
• Rodan, S.B. and Rodan, G.A.: The Effect of Parathyroid Hormone and Thyrocalcitonin on the Accumulation of Cyclic Adenosine 3'5'-monophosphate in Freshly Isolated Bone Cells, J Biol Chem 249:3068-3074, 1974.[Abstract/Free Full Text]
• Schuster, G.S.; Dirksen, T.R.; and Harms, W.S.: Effect of Exogenous Lipid on Lipid Synthesis by Bone and Bone Cell Cultures, J Dent Res 54:131-139, 1975.[Abstract/Free Full Text]
• Luben, R.A.; Wong, G.L.; and Cohn, D.V.: Biochemical Characterization with Parathormone and Calacitonin of Isolated Bone Cells: Provisional Identification of Osteoclasts and Osteoblasts, Endocrinology 99(2):526-534, 1976.[Abstract/Free Full Text]
• Wong, G.L. and Cohn, D.V.: The Effect of Parathormone on the Synthesis of Collagenous Matrix by Isolated Bone Cells. Proceedings, Mechanisms of Localized Bone Loss, Horton, Tarpley, and Davis, Eds., Sp. Suppl. to Calcified Tissue Abstracts 1974, pp. 47-84.
• Dziak, R. and Brand, J.S.: Calcium Transport in Isolated Bone Cells I. Calcium Transport Studies, J Cell Physiol 84:75-84, 1974.
• Dziak, R. and Brand, J.S.: Calcium Transport in Isolated Bone Cells II. Calcium Transport Studies, J Cell Physiol 84:85-96, 1974.[CrossRef][Medline] [Order article via Infotrieve]
• Dziak, R. and Stern, P.H.: Responses of Fetal Rat Bone Cells and Bone Organ Cultures to the Ionophore, A23187, Calcif Tiss Res 22:137-147, 1976.[CrossRef]
• Wong, G.L. and Cohn, D.V.: Separation of Parathyroid Hormone and Calcitonin-sensitive Cells from Non-responsive Bone Cells, Nature 252:713-715, 1974.[CrossRef][Medline] [Order article via Infotrieve]
• Wong, G.L. and Cohn, D.V.: Target Cells in Bone for Parathormone and Calcitonin are Different: Enrichment for Each Cell Type by Sequential Digestion of Mouse Calvaria and Selective Adhesion to Polymeric Surfaces, Proc Natl Acad Sci USA 72:3167-3171, 1975.[Abstract/Free Full Text]
• Luben, RA and Cohn, D.V.: Effects of Parathormone and Calcitonin on Citrate and Hyaluronate Metabolism in Cultured Bone, Endocrinology 98:413-419, 1976.[Abstract/Free Full Text]
• Heersche, J.N.M.; Moe, H.K.; Brunette, D.M.; Rao, A.V.; Riemers, S.; and Rao, L.B.: Long-term Culture of Parathyroid Hormone and Prostaglandin E1 Responsive Bone Cells in Monolayers and on Artificial Capillaries, Calcif Tiss Res 22 (suppl):275-282, 1977.[CrossRef]
• Puzas, J.E. and Brand, J.S.: Collagenolytic Activity from Isolated Bone Cells, Biochem Biophys Acta 429:964-974, 1976.[Medline] [Order article via Infotrieve]
• Smith, D.M.; Johnston, C.C.; and Sever-Son, A.R.: Studies of the Metabolism of Separated Bone Cells. I. Techniques of Separation and Identification, Calc Tiss Res 11: 59-96, 1973.
• Binderman, I.; Duksin, D.; Harell, A.; Katzir, E.; and Sachs, L.: Formation of Bone Tissue in Culture from Isolated Bone Cells, J Cell Biol 61:427-439, 1974.[Abstract/Free Full Text]
• Harell, A.; Dekel, S.; and Binderman, I.: Biochemical Effect of Mechanical Stress
• on Cultured Bone Cells, Calcif Tiss Res 22, (suppl):202-207, 1977.[CrossRef]
• Nelson, R.L. and Bauer, G.E.: Isolation of Osteoclasts by Velocity Sedimentation at Unit Gravity, Calcif Tiss Res 22:303-313, 1977.[CrossRef]
• Stern, B.D.: Collagen Solubility of Normal and Inflamed Human Gingivae, Periodontics 5:167-171, 1967.
• Lynch, C.; Lara-Braud, C.; and Con-Way T.: A Direct Assay for Collagen Synthesis Based on the Estimation of Collagenase-derived N-glycyl Peptides, Analytical Biochemistry 83:64-74, 1977.[CrossRef][Medline] [Order article via Infotrieve]
• Kirsten, E.S. and Watson, J.A.: Regulation of 3-hydroxy-3-methylglutaryl Coenzyme A Reductase in Hepatoma Tissue Culture Cells by Serum Lipoproteins, J Biol Chem 249(19):6104-6109, 1974.[Abstract/Free Full Text]
• Folch, J.; Lees, M.; and Stanley, G.H.: A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J Biol Chem 226:497-509, 1957.[Free Full Text]
• Kandutsch, AA and Saucier, S.E.: Prevention of Cyclic and Triton-induced Increases in Hydroxymethylglutaryl Coenzyme A Reductase and Sterol Synthesis by Puromycin, J Biol Chem 244(9):2299-2305, 1969.[Abstract/Free Full Text]
• Fieser, L.F.: Organic Experiments, Cholesterol, Boston, MA: D.C. Heath and Co., 1964, Chap. 12, pp. 72-73.
• Dietrich, J.W.; Goodson, J.M.; and Raisz, L.G.: Stimulation of Bone Resorption by Various Prostaglandins in Organ Culture. Prostaglandins 10:231-240, 1975.[Medline] [Order article via Infotrieve]
• Seibert, S.; Kadis, B.; and Bahn, A.: The Effects of Bacterial Endotoxins on Bone Cell Metabolism, J Cell Biol, Vol. 79:73a, 1978 (Abstract #CU326).
• Rasmussen, H. and Bordier, P.: The Physiological and Cellular Basis of Metabolic Bone Disease, Baltimore: Williams & Wilkins, 1974.
• Cameron, D.A.: The Ultrastructure of Bone. The Biochemistry and Physiology of Bone, Bourne, G.H., Ed., 2nd ed., 1972, Vol. 1, p. 191.[CrossRef]
• Dietrich, J.W.; Canalis, E.M.; Maina, D.M.; and Raisz, L.G.: Hormonal Control of Bone Collagen Synthesis in vitro: Effects of Parathyroid Hormone and Calcitonin, Endocrinology 98:943-949, 1976.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 59, No. 11, 2006-2013 (1980)
DOI: 10.1177/00220345800590111901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kadis, B. Articles by Seibert, S. Search for Related Content PubMed PubMed Citation Articles by Kadis, B. Articles by Seibert, S. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL Social Bookmarking                         What's this?