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Comparison of the Glucosyltransferases of Streptococcus mutans GS-5 to a Non-cariogenic Mutant

Department of Microbiology, Southern Illinois University, Carbondale, Illinois 62901

M. Craig Johnson

Department of Microbiology, Southern Illinois University, Carbondale, Illinois 62901

I.L. Shechmeister

Department of Microbiology, Southern Illinois University, Carbondale, Illinois 62901

The extracellular glucosyltransferase (GTF) enzymes of Streptococcus mutans GS-5 and avirulent mutant GS-511 were fractionated using agarose and DEAE-cellulose columns. GTF of GS-5 produced both water-soluble and -insoluble glucans, while those of GS-511 made soluble products almost exclusively. Nearly all (98%) of the small amount of insoluble GTF enzyme made by GS-511 was bound to the cell wall.

REFERENCES

• Montville, T.J.; Cooney, C.L.; and Sinskey, A.J.: Streptococcus mutans Dextransucrase: A Review, Adv Appl Microbiol 24:55-84, 1978.[CrossRef][Medline] [Order article via Infotrieve]
• Tanzer, J.M.; Freedman, M.L.; Fitzgerald, R.J.; and Lawson, R.H.: Diminished Virulence of Glucan Synthesis-defective Mutants of Streptococcus mutans Infect Immun 10: 197-203, 1974.
• Johnson, M.C.; Bozzola, J.J.; Shechmeister, I.L.; and Shklair, I.L.: Biochemical Study of the Relationship of Extracellular Glucan to Adherence and Cariogenicity in Streptococcus mutans and an Extracellular Polysaccharide Mutant, J Bacteriol 129:351-357, 1977.[Abstract/Free Full Text]
• Johnson, M.C.; Bozzola, J.J.; and Shechmeister, I.L.: Morphological Study of Streptococcus mutans and Two Extracellular Polysaccharide Mutants, J Bacteriol 118:304-311,1974.[Abstract/Free Full Text]
• Bozzola, J.J.; Johnson, M.C.; and Shechmeister, I.L.: Ultrastructural Localization of Sucrases in Streptococcus mutans GS-5 and an Extracellular Polysaccharide Mutant: A Comparative Cytochemical and Immunocytochemical Study, Infect Immun 17:447-457, 1977.[Abstract/Free Full Text]
• Mukasa, H. and Slade, H.D.: Mechanisms of the Adherence of Streptococcus mutans to Smooth Surfaces. III. Purification and Properties of the Enzyme Complex Responsible for Adherence, Infect Immun 10:1135-1145, 1974.[Abstract/Free Full Text]
• Hehre, E.J.: Polysaccharide Synthesis from Disaccharides, Methods Enzymol 1:179-184, 1955.
• Mukasa, H. and Slade, H.D.: Mechanism of Adherence of Streptococcus mutans to Smooth Surfaces. I. Roles of Insoluble Dextran-Levan Synthetase Enzymes and Cell Wall Polysaccharide Antigen in Plaque Formation, Infect Immun 8:555-562, 1973.[Abstract/Free Full Text]
• Fukui, K.; Fukui, Y.; and Moriyama, T.: Purification and Properties of Dextransucrase and Invertase from Streptococcus mutans, J Bacteriol 118:796-804, 1974.[Abstract/Free Full Text]
• Kuramitsu, H.K.: Characterization of Extracellular Glucosyltransferase Activity of Streptococcus mutans, Infect Immun 12: 738-749, 1975.[Abstract/Free Full Text]
• Schachtele, C.F.; Germaine, G.R.; and Harlander, S.K.: Production of Elevated Levels of Dextransucrase by a Mutant of Streptococcus mutans, Infect Immun 12: 934-937, 1975.[Abstract/Free Full Text]
• Lowry, O.H.: ROSEBROUGH, N.J.; FARR, A.L.; and RANDALL, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275, 1951.
• Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F.: Colorimetric Method for the Determination of Sugars and Related Substances, Anal Chem 28:350-356,1956.
• Kuramitsu, H.K.: Properties of a Mutant of Streptococcus mutans Altered in Glucosyltransferase Activity, Infect Immun 13:345-353, 1976.[Abstract/Free Full Text]
• Gibbons, R.J. and Fitzgerald, R.J.: Dextran-induced Agglutination of Streptococcus mutans and Its Potential Role in the Formation of Microbial Dental Plaques, J Bact 98:341-346, 1969.[Abstract/Free Full Text]
• Germaine, G.R.: CHLUDZINSKI, A.M.; and SCHACHTELE, C.F.: Streptococcus mutans Dextransucrase: Requirement for Primer Dextran, J Bact 120:289-294, 1974.
• Russell, R.B.: Glucan-binding Proteins of Streptococcus mutans Serotype C, J Gen Micro 112:197-201, 1979.
• Montville, T.J.; Cooney, C.L.; and Sinskey, A.J.: Measurement of Synthesis of Insoluble and Soluble Dextran by Streptococcus mutans, J Dent Res 56:983-989, 1977.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 59, No. 10, 1613-1619 (1980)
DOI: 10.1177/00220345800590101001


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Bozzola, J. J. Articles by Shechmeister, I.L. Search for Related Content PubMed PubMed Citation Articles by Bozzola, J. J. Articles by Shechmeister, I.L. Social Bookmarking                         What's this?