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Journal of Dental Research
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The Concentrations of Free Fatty Acids, Triglycerides and Phospholipids in Submandibular Salivary Glands of Rats in Essential Fatty Acid Deficiency

Syed Q. Alam

Department of Biochemistry, LSU Medical Center, New Orleans, La. 70119, U.S.A

Bassima S. Alam

Department of Biochemistry, LSU Medical Center, New Orleans, La. 70119, U.S.A

EFA deficiency was created in young weanling rats by feeding for 16 weeks purified diets containing 0% fat or 7% hydrogenated coconut oil (HCO). Control rats were fed similar diets which contained 7% corn oil or 5% HCO + 2% corn oil. Submandibular salivary gland (SMSG) lipids were extracted and analyzed for the concentrations of the free fatty acids (FFA), triglycerides (TG), total lipid P and the proportions of various phospholipids. In EFA-deficient SMSG, the concentrations of FFA and TG fractions were lower compared to those of the controls. Total lipid P was either unaffected or slightly increased; the proportion of phospholipids was not changed. Possible reasons for these changes in SMSG lipids are discussed.

REFERENCES

  • Mead, J.F.: The metabolism of the essential fatty acids. VI: Distribution of unsaturated fatty acids in rats on a fat-free and supplemental diets, J Biol Chem 227:1025-1034, 1957.[Free Full Text]
  • Mohrhauer, H.; and Holman, R.T.: The effect of dietary essential fatty acids upon composition of polyunsaturated fatty acids in depot fat and erythrocytes of the rat, J Lipid Res 4:346-350, 1963.[Abstract]
  • Sand, D.; Sen, N.; and Schlink, H.: Positional isomerism of unsaturated fatty acids in the rat and quantification of isomeric mixtures, J Amer Oil Chem Soc 42:511-516, 1965.[Medline] [Order article via Infotrieve]
  • Walker, B.L.: Recovery of rat tissue lipids from essential fatty acids deficiency: plasma, erythrocytes and liver, J Nutr 92:23-39, 1967.[Abstract/Free Full Text]
  • Fulco, A.; and Mead, J.F.: Metabolism of essential fatty acids. VIII. Origin of 5,8,11-eicosatrienoic acid in the fat-deficient rat, J Biol Chem 234:1411-1416, 1959.[Free Full Text]
  • Holman, R.T.: The ratio of trienoic: tetranenoic acid as a measure of essential fatty acid requirement, J Nutr 70:405-410, 1960.[Abstract/Free Full Text]
  • Prout, R.E.S.; and Atkins, E.R.: Effect of diet deficient in essential fatty acid on fatty acid composition of enamel and dentin of the rat, Arch Oral Biol 18:583-590,1973.[Medline] [Order article via Infotrieve]
  • Reid, M.E.; Bieri, J.G.; Plack, P.A.; and Andrews, E.L.: Nutritional studies with the guinea pig. X. Determination of the linoleic acid requirement, J Nutr 82:401-408, 1964.[Abstract/Free Full Text]
  • Lekholm, U.: Effects of essential fatty acid deficiency and of carbon tetrachloride-induced liver cirrhosis on lipid and fatty acid composition of oral palatal epithelium in rats, Int J Oral Surg 5:311-321, 1976.[Medline] [Order article via Infotrieve]
  • Holman, R.T.: Essential fatty acid deficiency, Prog Chem Fats 9:279-348, 1968.
  • Alam, S.Q.; and Alam, B.S.: Fatty acid composition of submandibular salivary glands lipids in essential fatty acid deficient rats, JNutr, 108:1642-1651, 1978.[Abstract/Free Full Text]
  • Folch, J.; Lees, M.; and Sloane-Stanley, G.H.: A simple method for the isolation and purification of total lipids from animal tissues, J Biol Chem 226:497-509, 1957.[Free Full Text]
  • Rouser, G.; Kritchevesky, G.; and Yamamato, A.: Column chromatographic and associated procedures for separation and determination of phosphatides and glycolipids. In: Lipid Chromatographic Analysis (Edited by Marinetti, G.V. ), New York, Dekker, 1967, Vol 1, pp 118-120.
  • Alam, S.Q.; and Alam, B.S.: Effect of isoprenaline on salivary gland lipids of rats fed a choline-deficient diet, J Dent Res 57:653-649,1978.
  • Bartlett, G.R.: Phosphorus assay in column chromatography, J Biol Chem 234: 466-468, 1959.[Free Full Text]
  • Van Drop, D.A.: Recent developments in the biosynthesis and the analyses of prostaglandins, Ann N Y Acad Sci 180:181-199, 1971.
  • Hittelman, K.J.; and Butcher, R.W.: Cyclic AMP and the mechanism of action of the prostaglandins. In: The Prostaglandins (Cuthbert, M. F., ed.) pp 151-165, Lippincott, J. P., Philadelphia, 1973.
  • Sun, G.Y.: Effects of fatty acid deficiency on lipids of whole brain, microsomes, and myelin in the rat, J Lipid Res 13:56-62, 1972.[Medline] [Order article via Infotrieve]
  • Kyriakides, E.C.; Beeler, D.A.; Ed-Monds, R.H.; and Balint, J.A.: Alterations in phosphatidyl choline species and their reversal in pulmonary surfactant during essential fatty acids deficiency, Biochim Biophys Acta 431:399-407, 1976.[Medline] [Order article via Infotrieve]
  • Stancliff, R.C.; Williams, M.A.; Utsumi, K.; and Packer, L.: Essential fatty acids deficiency and mitochondrial function, Arch Biochem Biophys 131:629-642, 1969.[CrossRef][Medline] [Order article via Infotrieve]
  • Morton, R.A.; and Horner, A.A.: The liver-lipid constituents of male and female rats I. Effects of the fat-deficiency syndrome. Biochem J 79:631-635, 1961.[Medline] [Order article via Infotrieve]
  • Bergstrom, S.; Danielsson, H.; and Samuelsson, B.: The enzymatic formation of prostaglandin E2 from arachidonic acid, Biochim Biophys Acta 90:207-210, 1964.[Medline] [Order article via Infotrieve]
  • Angaard, E.; and Samuelsson, B.: Biosynthesis of prostaglandins from arachidonic acid in guinea pig lung, J Bio Chem 240:3518-3521, 1965.[Free Full Text]
  • Hahn, R.A.; and Patel, P.N.: Salivation induced by prostaglandin F2{alpha} and modification of the response of atropine and physostigmine, Br J Pharmacol 44:527-533, 1972.
  • Hahn, R.A.; and Patel, P.N.: Further observations on the interaction of prostaglandin F2{alpha} with cholinergic mechanisms in canine salivary glands, Eur J Pharmacol 25:279-286,1974.[Medline] [Order article via Infotrieve]
  • Snedecor, G.W.; and Cochran, W.G.: Statistical Methods, The Iowa State University Press, Ames, Iowa, 1967.

Journal of Dental Research, Vol. 58, No. 6, 1640-1643 (1979)
DOI: 10.1177/00220345790580061401


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