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Polyacrylamide Gel Electrophoresis of Human Salivary Histidine-Rich-Polypeptides
Bruce J. Baum
Dental Sciences Department, Naval Medical Research Institute, Bethesda Maryland 20014, USA
Janice L. Bird
Dental Sciences Department, Naval Medical Research Institute, Bethesda Maryland 20014, USA
Robert W. Longton
Dental Sciences Department, Naval Medical Research Institute, Bethesda Maryland 20014, USA
A method of polyacrylamide gel electrophoresis for examining histidine-rich-polypeptides in human saliva is described. Comparison is made to several commonly used electrophorretic techniques. The described method allows for the resolution of seven histidine-rich-polypeptide fractions and is convenient and quite reproducible.
REFERENCES
- Mandel, I.D.: Electrophoretic Studies of Saliva, J Dent Res 45:634-643, 1966.[Free Full Text]
- Baum, B.J.; Bird, J.L.; Millar, D.B.; and Longton, R.W.: Studies on Histidine-Rich-Polypeptides from Human Parotid Saliva, Arch Biochem Biophys 177:427-436, 1976.[CrossRef][Medline]
[Order article via Infotrieve]
- Hay, D.I.: Fractionation of Human Salivary Proteins and the Isolation of an Histidine-Rich-Acidic Peptide Which Shows High Affinity for Hydroxyapatite Surfaces, Arch Oral Biol 20:553-558, 1975.[CrossRef][Medline]
[Order article via Infotrieve]
- Holbrook, I.B., and Molan, P.C.: The Identification of a Peptide in Human Parotid Saliva Particularly Active in Enhancing the Glycolytic Activity of Salivary Microorganisms, Biochem J 119:489-492, 1975.
- Azen, E.A.: Genetic Polymorphism of Basic Proteins from Parotid Saliva, Science 176: 673-674, 1972.[Abstract/Free Full Text]
- Davis, B.J.: Disc Electrophoresis. II. Method and Application to Human Serum Proteins, Ann NY Acad Sci 121:404-427, 1964.[Medline]
[Order article via Infotrieve]
- Reisfeld, R.A.; Lewis, U.J.; and Wil-Liams, D.E.: Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels, Nature 195:281-283, 1962.[CrossRef][Medline]
[Order article via Infotrieve]
- Panyim, S., and Chalkey, A.: High Resolution Acrylamide Gel Electrophoresis of Histones, Arch Biochem Biophys 130:337-346, 1969.[CrossRef][Medline]
[Order article via Infotrieve]
- Furthmayr, H., and Timpl, R.: Characterization of Collagen Peptides by Sodium Dodecylsulfate-Polyacrylamide Electrophoresis, Anal Biochem 41:510-516, 1971.[CrossRef][Medline]
[Order article via Infotrieve]
- Bonilla, C.A., and Stringham, JR, R.M.: Electrophoresis of Human Salivary Secretions at Acid pH, J Chromatog 50:345-348, 1970.[CrossRef][Medline]
[Order article via Infotrieve]
- Azen, E.A.: Properties of Salivary Basic Proteins Showing Polymorphism, Biochem Genet 9:69-86, 1973.[CrossRef][Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 56, No. 9,
1115-1118 (1977)
DOI: 10.1177/00220345770560091801
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