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Journal of Dental Research
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Lactose Synthase Activity of Galactosyltransferase from Human Parotid Saliva

R. Nakamura

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

E. Iwasaki

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

S. Shibata

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

T. Matsumura

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

S. Shizukuishi

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

A. Tsunemitsu

Department of Preventive Dentistry, Osaka University Dental School, Osaka 530, Japan

Galactosyltransferase in human parotid saliva catalyzed the transfer of galactose from uridine 5'-diphospho (UDP)-galactose to D-glucose in the presence of exogenous a-lactalbumin. Some albumins other than {alpha}-lactalbumin did not have an effect on the appearance of lactose-synthesizing activity.

REFERENCES

  • Nakamura, R.; Watanabe T.; Yasutake, A.; and Tsunemitsu, A.: The Presence of Uridine-5'-Diphosphogalactose: N-Acetylglucosamine Galactosyltransferase in Human Saliva, J Dent Res 53:937, 1974.[Free Full Text]
  • Nakamura, R.; Watanabe, T.; and Tsunemitsu, A.: Some Properties of Uridine 5'-Diphosphogalactose: N-Acetylglucosamine Galactosyltransferase in Human Parotid Saliva, J Dent Res 54:808-813, 1975.[Abstract/Free Full Text]
  • Brodbeck, U.; Denton, W.L.; Tanahashi, N.; and Ebner, K.E.: The Isolation and Identification of the B Protein of Lactose Synthetase as {alpha}-Lactalbumin, J Biol Chem 242:1391-1397, 1967.[Abstract/Free Full Text]
  • Brew, K.; Vanaman, T.C.; and Hill, R.L.: The Role of a-Lactalbumin and the A Protein in Lactose Synthetase: A Unique Mechanism for the Control of a Biological Reaction, Proc Natl Acad Sci USA 59:491-497, 1968.[Free Full Text]
  • Morrison, J.F., and Ebner, K.E.: Studies on Galactosyltransferase: Kinetic Effects of a-Lactalbumin with N-Acetylglucosamine and Glucose as Galactosyl Group Acceptors, J Biol Chem 246:3992-3998, 1971.[Abstract/Free Full Text]
  • Watanabe, T.; Nakamura, R.; and Tsunemitsu, A.: Transglycosylation by the Exo-Type of β-N-Acetylglucosaminidase from Human Parotid Saliva, Arch Oral Biol 20:393-394, 1975.[CrossRef][Medline] [Order article via Infotrieve]
  • Brodbeck, U., and Ebner, K.E.: Resolution of a Soluble Lactose Synthetase into Two Protein Components and Solubilization of Microsomal Lactose Synthetase, J Biol Chem 241:762--764, 1966.[Abstract/Free Full Text]
  • Iwamoto, Y.; Nakamura, R.; Watanabe, T.; and Tsunemitsu, A.: Purification and Amino Acid Analysis of Human Parotid Saliva Lysozyme, J Dent Res 49:1104-1110, 1970.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 56, No. 2, 181-184 (1977)
DOI: 10.1177/00220345770560021301


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R. Nakamura, T. Taniguchi, H. Nonaka, S. Shizukuishi, and A. Tsunemitsu
Purification and Characterization of Galactosyltransferase in Human Parotid Saliva
Journal of Dental Research, August 1, 1980; 59(8): 1374 - 1381.
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This Article
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