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Journal of Dental Research
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Proteolipid and Calculus Matrix Calcification In Vitro

J. Ennever

Dental Science Institute, The University of Texas Dental Branch, Houston, Texas 77030, USA

J.J. Vogel

Dental Science Institute, The University of Texas Dental Branch, Houston, Texas 77030, USA

L.J. Riggan

Dental Science Institute, The University of Texas Dental Branch, Houston, Texas 77030, USA

S.B. Paoloski

Dental Science Institute, The University of Texas Dental Branch, Houston, Texas 77030, USA

The initiator of calculus matrix calcification, in vitro, was isolated. Crude phospholipid, known to contain the factor, was separated into five fractions by column chromatography. A single protein-containing fraction induced apatite formation during incubation in a metastable calcium phosphate solution. The nucleating fraction was identified as a proteolipid.

REFERENCES

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  • Ennever, J.: Unpublished data.
  • Vogel, J.J.: Campbell, M.M.; and Ennever, J.: Calcification of a Lysozyme-Inositol Phosphatide Complex In Vitro, Proc Soc Exp Biol Med 143:677-680, 1973.[CrossRef][Medline] [Order article via Infotrieve]
  • Ennever, J.; Vogel, J.J.; Rider, L.J.; and Boyan-Salyers, B.: Nucleation of Micro-biologic Calcification by Proteolipid, Proc Soc Exp Biol Med 152:147-150, 1976.[CrossRef][Medline] [Order article via Infotrieve]
  • Ennever, J.; Vogel, J.J.; and Streck-Fuss, J.L.: Synthetic Medium for Calcification of Bacterionema matruchotii, J Dent Res 50:1027-1030, 1971.
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  • Rizzo, A.A.; Scott, D.B.; and Bladen, H.A.: Calcification of Oral Bacteria, Ann NY Acad Sci. 109:14-22, 1963.[CrossRef][Medline] [Order article via Infotrieve]
  • Ennever, J.; Vogel, J.J.; and Brown, L.R.: Survey of Microorganisms for Calcification in a Synthetic Medium, J Dent Res 51:1483-1486, 1972.[Abstract/Free Full Text]
  • Nicot, C.; Ngyen LE, T.; Leprêtre, M.; and Alfsen, A.: Study of Folch-Pi Apoprotein: I. Isolation of Two Components, Aggregation During Delipidation, Biochim Biophys Acta 32:109-123, 1973.
  • Vogel, J.J.; Campbell, M.M.; and Ennever, J.: Characteristics of Calcifiable Lipoprotein, J Dent Res (SpecialIssueA) 54: Abstract No. 319, 1974.
  • Zander, H.A.; Hazen, S.P.; and Scott, D.B.: Mineralization of Dental Calculus, Proc Soc Exp Biol Med 103:257-260, 1960.[CrossRef][Medline] [Order article via Infotrieve]
  • Gonzales, F., and Sognnaes, F.F.: Electronmicroscopy of Dental Calculus, Science 131:156-158, 1960.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 56, No. 2, 140-142 (1977)
DOI: 10.1177/00220345770560020701


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This Article
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