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Journal of Dental Research
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Extracellular Dextran Hydrolase from Streptococcus mutans Strain 6715

David W. Ellis

Laboratory of Oral Microbiology, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA

Chris H. Miller

Laboratory of Oral Microbiology, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA

Streptococcus mutans strain 6715 was shown to produce an extracellular dextranase with endohydrolytic {alpha}-(1->6)-glucan-6-glucanohydrolase activity. The enzyme degraded soluble polymers produced by some oral streptococci but did not disperse the streptococcal plaques tested. Enzyme levels in the culture supernatant were elevated in sucrose and sucrose plus glucose cultures but remained at basal levels in glucose and dextran plus glucose cultures.

REFERENCES

  • Walker, G.J.: Some Properties of a Dextranglucosidase Isolated from Oral Streptococci and Its Use in Studies on Dextran Synthesis, J Dent Res 51:409-414, 1972.[Free Full Text]
  • Staat, R.H.; Gawronski, T.H.; and Schachtele, C.F.: Detection and Preliminary Studies on Dextranase-Producing Microorganisms from Human Dental Plaque, Infec Immun 8:1009-1016, 1973.[Abstract/Free Full Text]
  • Schachtele, C.F.; Staat, R.H.; and Har-Lander, S.K.: Dextranases from Oral Bacteria: Inhibition of Water-Insoluble Glucan Production and Adherence to Smooth Surfaces by Streptococcus mutans, Infect Immun 12:309-317, 1975.[Abstract/Free Full Text]
  • Mäkinen, K.K., and Paunio, I.K.: Exploitation of Blue Dextran as a Dextranase Substrate, Anal Biochem 39: 202-207, 1971.[CrossRef][Medline] [Order article via Infotrieve]
  • Staat, R.H., and Schachtele, C.F.: Evaluation of Dextranase Production by the Cariogenic Bacterium Streptococcus mutans, Infect Immun 9:467-469, 1974.[Abstract/Free Full Text]
  • Guggenheim, B., and Burkhardt, J.J.: Isolation and Properties of a Dextranase from Streptococcus mutans OMZ 176, Helv Odontol Acta 18:101-113, 1974.[Medline] [Order article via Infotrieve]
  • Dewar, M.D., and Walker, G.J.: Metabolism of the Polysaccharides of Human Dental Plaque: I. Dextranase Activity of Streptococci, and the Extracellular Polysaccharides Synthesized from Sucrose, Caries Res 9:21-35, 1975.[Medline] [Order article via Infotrieve]
  • Da Costa, T.; Bier, L.C.; and Gaida, F.: Dextran Hydrolysis by a Fusobacterium Strain Isolated from Human Dental Plaque, Arch Oral Biol 19: 341-342, 1974.[CrossRef][Medline] [Order article via Infotrieve]
  • Jordan, H.V.; Fitzgerald, R.J.; and Bowler, A.E.: Inhibition of Experimental Caries by Sodium Metabisulfite and Its Effect on the Growth and Metabolism of Selected Bacteria, J Dent Res 39:116-123, 1960.[Free Full Text]
  • Lowry, O.H.; Rosenbrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275, 1951.[Free Full Text]
  • Somogyi, M.: Determination of Blood Sugar, J Biol Chem 160:69-73, 1945.[Free Full Text]
  • Dubois, M.; Giles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F.: Colorimetric Method for Determination of Sugars and Related Substances, Anal Chem 28: 350-354, 1956.
  • Destefanis, V.A., and Ponte, J.G., JR.: Separation of Sugars by Thin-Layer Chromatography, J Chromatogr 34: 116-120, 1968.[CrossRef]
  • Mccabe, R.M.; Keyes, P.H.; and Howell, A., JR.: An In Vitro Method for Assessing the Plaque Forming Ability of Oral Bacteria, Arch Oral Biol 12:1653-1656, 1967.[CrossRef][Medline] [Order article via Infotrieve]
  • Fitzgerald, R.J.; Keyes, P.H.; Stoudt, T.H.; and Spinell, D.M.: The Effects of Dextranase Preparation on Plaque and Caries in Hamsters: A Preliminary Report, JADA 76:301-304.
  • Gibbons, R.J., and Fitzgerald, R.J.: Dextran-Induced Agglutination of Streptococcus mutans, and Its Potential Role in the Formation of Microbial Dental Plaques, J Bacteriol 98:341-346, 1969.[Abstract/Free Full Text]
  • Gibbons, R.J., and Nygaard, M.: Inter-bacterial Aggregation of Plaque Bacteria, Arch Oral Biol 15:1397-1400, 1970.[CrossRef][Medline] [Order article via Infotrieve]
  • Greer, S.B.; Hsiang, W.; Musil, G.; and Zinner, D.D.: Viruses of Cariogenic Streptococci, J Dent Res 50:1594-1604, 1971.[Free Full Text]
  • Van Houte, J.; Gibbons, R.J.; and Pulk-Kinen, A.J.: Adherence as an Ecological Determinant for Streptococci in the Human Mouth, Arch Oral Biol 16:1131-1141, 1971.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons, R.J.: Presence of an Invertase-Like Enzyme and a Sucrose Permeation System in Strains of Streptococcus mutans, Caries Res 6:122-131, 1972.[Medline] [Order article via Infotrieve]
  • Schachtele, C.F.; Loken, A.E.; and Knudson, D.J.: Preferential Utilization of the Glucose Moiety of Sucrose by a Cariogenic Strain of Streptococcus mutans, Infect Immun 5:531-536, 1972.[Abstract/Free Full Text]
  • Evans, R.T., and Genco, R.J.: Inhibition of Glucosyltransferase Activity by Antisera to Known Serotypes of Streptococcus mutans, Infect Immun 7:237-241, 1973.[Abstract/Free Full Text]
  • Pitts, G., and Keele, B.B., JR.: Bacterial Degradation of Streptococcal Glucans, J Dent Res 52:1303-1309, 1973.[Abstract/Free Full Text]
  • Tanzer, J.M.; Brown, A.T.; and McInerney, M.F.: Identification, Preliminary Characterization, and Evidence for Regulation of Invertase in Streptococcus mutans, J Bacteriol 116:192-202, 1973.[Abstract/Free Full Text]
  • Miller, C.H., and Kleinman, J.L.: Effect of Microbial Interactions on In Vitro Plaque Formation by Streptococcus mutans, J. Dent Res 53:427-434, 1974.[Abstract/Free Full Text]
  • Germaine, G.R.; Schachtele, C.F.; and Chludzinski, A.M.: Rapid Filter Paper Assay for the Dextransucrose Activity from Streptococcus mutans, J Dent Res 53:1355-1360, 1974.[Abstract/Free Full Text]
  • Chludzinski, A.M.; Germaine, G.R.; and Schachtele, C.F.: Purification and Properties of Dextransucrose from Streptococcus mutans, J Bacteriol 118:1-7, 1974.[Abstract/Free Full Text]
  • Freedman, M.L.; and Tanzer, J.L.: Dissociation of Plaque Formation from Glucan-Induced Agglutination in Mutants of Streptococcus mutans, Infect Immun 10:189-196, 1974.[Abstract/Free Full Text]
  • Tanzer, J.L.; Freedman, M.L.; Fitzgerald, R.J.; and Larson, R.H.: Diminished Virulence of Glucan Synthesis-Defective Mutants of Streptococcus mutans, Infect Immun 10: 197-203, 1974.[Abstract/Free Full Text]
  • Linzer, R., and Slade, H.D.: Purification and Characterization of Streptococcus mutans Group d Cell Wall Polysaccharide Antigen, Infect Immun 10:361-368, 1974.[Abstract/Free Full Text]
  • Germaine, G.R.; Chludzinski, A.M.; and Schachtele, C.F.: Streptococcus mutans Dextransucrase: Requirement for Primer Dextran, Infect Immun 120:287-294, 1974.
  • Streckfuss, J.L.; Smith, W.N.; Brown, L.R.; and Campbell, M.M.: Calcification of Selected Strains of Streptococcus mutans and Streptococcus sanguis, J Bacteriol 120: 502-506, 1974.[Abstract/Free Full Text]
  • Nalbandian, J.; Freedman, M.L.; Tanzer, J.M.; and Lovelace, S.M.: Ultrastructure of Mutants of Streptococcus mutans with Reference to Agglutination, Adhesion, and Extracellular Polysaccharides, Infect Immun 10:1170-1179, 1974.
  • Spinell, D.M., and Gibbons, R.J.: Influence of Culture Medium on the Glucosyl Transferase- and Dextran-Binding Capacity of Streptococcus mutans 6715 Cells, Infect Immun 10:1448-1451, 1974.[Abstract/Free Full Text]
  • Schachtele, C.F.: Glucose Transport in Streptococcus mutans: Preparation of Cytoplasmic Membranes and Characteristics of Phosphotransferase Activity, J Dent Res 54: 330-338, 1975.[Medline] [Order article via Infotrieve]
  • Iacono, V.J.; Taubman, M.A.; Smith, D.J.; and Levine, M.J.: Isolation and Immunochemical Characterization of the Group-Specific Antigen of Streptococcus mutans 6715, Infect Immun 11: 117-128, 1975.[Abstract/Free Full Text]
  • Terleckyj, B.; Willett, N.P.; and Shockman, G.D.: Growth of Several Strains of Oral Streptococci in a Chemically Defined Medium, Infect Immun 11:649-655, 1975.[Abstract/Free Full Text]
  • Terleckyj, B., and Shockman, G.D.: Amino Acid Requirements of Streptococcus mutans and Other Oral Streptococci, Infect Immun 11:656-664, 1975.[Abstract/Free Full Text]
  • Michalek, S.M.; McGhee, J.R.; and Navia, J.M.: Virulence of Streptococcus mutans: A Sensitive Method for Evaluating Cariogenicity in Young Gnotobiotic Rats, Infect Immun 12:69-75, 1975.[Abstract/Free Full Text]
  • Fitzgerald, R.J.; Spinell, D.M.; and Stoudt, T.H.: Enzymatic Removal of Artificial Plaques, Arch Oral Biol 13:125-128, 1968.[CrossRef][Medline] [Order article via Infotrieve]
  • König, K.G., and Guggenheim, B.: In Vivo Effects of Dextranase on Plaque and Caries, Helv Odontol Acta 12:48-55, 1968.[Medline] [Order article via Infotrieve]
  • Guggenheim, B.; König, K.G.; Mühlemann, H.R.; and Regolati, B.: Effects of Dextranase on Caries in Rats Harbouring an Indigenous Cariogenic Bacterial Flora, Arch Oral Biol 14:555-558, 1969.[CrossRef][Medline] [Order article via Infotrieve]
  • Block, P.L.; Dooley, C.L.; and Howe, E.E.: The Retardation of Spontaneous Periodontal Disease and the Prevention of Caries in Hamsters with Dextranase, J Periodontol 40:41-46, 1969.
  • Bowen, W.H.: The Effect of Dextranase on Caries Activity in Monkeys (Macaca irus), Br Dent J 131:445-449, 1971.[CrossRef][Medline] [Order article via Infotrieve]
  • Lobene, R.R.: A Clinical Study of the Effect of Dextranase on Human Dental Plaque, J ADA 82:132-135, 1971.
  • Caldwell, R.C.; Sandham, H.J.; Mann, W.V., JR.; Finn, S.B.; and Formicola, A.J.: The Effect of Dextranase on Human Dental Plaque, J ADA 82:124-131, 1971.
  • Keyes, P.H.; Hicks, M.A.; Goldman, B.M.; Mccabe, R.M.; and Fitzgerald, R.J.: Dispersion of Dextranous Bacterial Plaques on Human Teeth with Dextranase, JADA 82:136-141, 1971.
  • Nyman, S.; Lindhf, J.; and Janson, J.: The Effect of a Bacterial Dextranase on Human Dental Plaque Formation and Gingivitis Development, Odontol Revy 23:243-252, 1972.[Medline] [Order article via Infotrieve]
  • Guggenheim, B.; Regolati, B.; and Mühlemann, H.R.: Caries and Plaque Inhibition by Mutanase in Rats, Caries Res 62:289-297, 1972.
  • Minah, G.E.; Loesche, W.J.; and Dzie-Wiatkowski, D.D.: The In Vitro Effect of Fungal Dextranase on Human Dental Plaque, Arch Oral Biol 17:35-42, 1972.[CrossRef][Medline] [Order article via Infotrieve]
  • Murayamr, Y.; Wada, H.; Hayashi, H.; Uchida, T.; Yokomizo, I.; and Hamada, S.: Effects of Dextranase from Spicaria violaceae (IFO 6120) on the Polysaccharides Produced by Oral Streptococci and on Human Dental Plaque, J Dent Res 52:658-667, 1973.[Abstract/Free Full Text]
  • Gibbons, R.J., and Banghart, S.B.: Synthesis of Extracellular Dextran by Cariogenic Bacteria and Its Presence in Human Dental Plaque, Arch Oral Biol 12:11-24, 1967.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons, R.J., and Nygaard, M.: Synthesis of Insoluble Dextran and Its Significance in the Formation of Gelatinous Deposits by Plaque-Forming Streptococci, Arch Oral Biol 13:1249-1262, 1968.[CrossRef][Medline] [Order article via Infotrieve]
  • Guggenheim, B.: Enzymatic Hydrolysis and Structure of Water-Insoluble Glucan Produced by Glucosyltransferases from a Strain of Streptococcus mutans, Helv Odontol Acta 5:89-108, 1970.
  • Holtz, P.; Guggenheim, B.; and Schmid, R.: Carbohydrates in Pooled Dental Plaque, Caries Res 6: 103-121, 1972.[Medline] [Order article via Infotrieve]
  • Ebisu, S.; Misaki, A.; Kato, K.; and Kotani, S.: The Structure of Water-Insoluble Glucans of Cariogenic Streptococcus mutans, Formed in the Absence and Presence of Dextranase, Carbohydr Res 38:374-381, 1974.[CrossRef][Medline] [Order article via Infotrieve]
  • Tsuchiya, H.M.; Jeannes, A.; Bricker, H.M.; and Wilham, C.A.: Dextran-Degrading Enzymes from Molds, J Bacteriol 64: 513-519, 1952.[Free Full Text]
  • Chaiet, L.; Kempf, A.S.; Harman, R.; Eaczka, E.; Weston, R.; Nallstadt, K.; and Wolf, F.: Isolation of A Pure Dextranase from Penicillium funiculosum, Appl Microbiol 20:421-426, 1970.[Medline] [Order article via Infotrieve]
  • Guggenheim, B., and Rosemarie, H.: Purification and Properties of Alpha (1, 3) Glucanohydrolase from Trichoderma harzianum, J Dent Res 51:394-402, 1972.[Free Full Text]
  • Staat, R.H., and Schachtele, C.F.: Characterization of a Dextranase Produced by an Oral Strain of Actinomyces israelii, Infect Immun 12:556-563, 1975.[Abstract/Free Full Text]
  • Ebert, K.H., and Brosche, M.: Origin of Branches in Native Dextrans, Biopolymers 5:123-130,1967.[CrossRef][Medline] [Order article via Infotrieve]
  • Ebisu, S.; Misaki, A.; and Kotani, S.: The Structure of Water-Insoluble Glucans of Cariogenic Streptococcus mutans, Formed in the Absence and Presence of Dextranase, Carbohydr Res 38:374-381, 1974.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 56, No. 1, 57-69 (1977)
DOI: 10.1177/00220345770560011301
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