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Journal of Dental Research
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Some Characteristics of Collagenase Activity in Gingival Crevicular Fluid and Its Relationship to Gingival Diseases in Humans

Lorne M. Golub

School of Dental Medicine, State University of New York, Stony Brook, New York 11794, USA, Columbia University School of Dental and Oral Surgery, New York, New York 10032

Kenneth Siegel

School of Dental Medicine, State University of New York, Stony Brook, New York 11794, USA, Columbia University School of Dental and Oral Surgery, New York, New York 10032

N.S. Ramamurthy

School of Dental Medicine, State University of New York, Stony Brook, New York 11794, USA, Columbia University School of Dental and Oral Surgery, New York, New York 10032

Irwin D. Mandel

School of Dental Medicine, State University of New York, Stony Brook, New York 11794, USA, Columbia University School of Dental and Oral Surgery, New York, New York 10032

Collagenase activity in human gingival fluid was measured using a radioactive collagen fibril assay. The activity was positively correlated with the severity of gingival disease. The fluid collagenase seemed to be controlled by {alpha}2-macroglobulin, based on its activation by NaSCN, and to be present solely in the extracellular fraction. Examination of the collagen breakdown products by acrylamide gel electrophoresis indicated that the fluid collagenase was of tissue rather than bacterial origin.

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Journal of Dental Research, Vol. 55, No. 6, 1049-1057 (1976)
DOI: 10.1177/00220345760550060701
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