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Observations on Calcium-Induced Stability of Salivary Proteins in a Lipid-Extraction System

Pediatric Dentistry, School of Dentistry and School of Medicine, University of California, Los Angeles, California 90024, USA

Modification of a lipid extraction method was used to demonstrate the ability of calcium to stabilize salivary proteins in an aqueous solution. Gel electrophoretic patterns showed that slowly migrating components were most affected. Several biologically active factors are implicated to be among those stabilized.

REFERENCES

• Pigman, W.: Some Recent Developments in the Composition and Physiology of Human Saliva, JADA 54: 469-472, 1957.
• Oppenheim, F.G., and Hay, D.I.: Further Studies of Human Serum Albumin in Oral Fluids, Helv Odontol Acta 16: 22-26, 1972.[Medline] [Order article via Infotrieve]
• Von Hippel, P.H., and Schleich, T.W.: The Effects of Neutral Salts on the Structure and Conformational Stability of Macromolecules in Solution, in Fasman, G.D., and Timasheff, S.N. (eds): Structure and Stability of Biological Macromolecules, Vol 2, New York: Marcel Decker, Inc., 1969, pp 417-574.
• Ostwald, W.: Über feinere Quellungsercheinungen von Gelatine in Salzlösungen nebst allgemeineren Bemerkungen zur physilkalish-chemischen Analyse de Quellungskuren in Electrolyten, Pfluegers Arch 111: 581-606, 1906.
• Edsall, J.T., and Wyman, J.: Biophysical Chemistry Vol 1, New York: Academic Press, Inc., 1958, pp 263-282.
• Grøn, P.: The Demonstration of a Dicalcium Phosphate Stabilizing Factor in Human Saliva, Arch Oral Biol 18: 1379-1383, 1973.[Medline] [Order article via Infotrieve]
• Grøn, P.: The State of Calcium and Inorganic Orthophosphate Stabilizing Factor in Human Saliva, Arch Oral Biol 18: 1365-1378, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Dreisbach, R.H.: Calcium Binding by Normal Human Saliva, J Dent Res 39: 1133-1140, 1960.[Free Full Text]
• Dirksen, T.R.: Lipid Constituents of Whole and Parotid Saliva, J Dent Res 42: 920-924, 1963.[Free Full Text]
• Manly, R.J., and Manly, K.F.: Influence of Cephaline on Solution Rate of Tooth Enamel, J Dent Res 42: 565-567, 1963.[Free Full Text]
• Icotmore, D.M.; Nichols, G., JR.; and Wuthier, R.E.: Phospholipid-Calcium Phosphate Complex: Enhanced Calcium Migration in the Presence of Phosphate, Science 172: 1339-1341, 1971.[Abstract/Free Full Text]
• Starcher, B.C., and Urry, D.W.: Elastin Coacervate As a Matrix for Calcification, Biochem Biophys Res Comm 53: 210-216, 1973.[CrossRef][Medline] [Order article via Infotrieve]
• Peterson, E.A., and Chiazze, E.A.: Some Experimental Factors in the Gradient Chromatography of Serum Proteins, Arch Biochem Biophys 99: 136-147, 1962.[Medline] [Order article via Infotrieve]
• Folch, J., and Lees, M.: Proteolipides: A New Type of Tissue Lipoproteins, J Biol Chem 191:807-817, 1951.[Free Full Text]
• Folch, J.; Ascoli, I.; Lees, M.; Meath, J.A.; and Lebaron, F.N.: Preparation of Lipide Extracts from Brain Tissue, J Biol Chem 191: 833-841, 1951.[Free Full Text]
• Tonzetich, J., and Friedman, S.D.: The Regulation of Metabolism by the Cellular Elements of Saliva, Ann NY Acad Sci 131: 815-829, 1965.[Medline] [Order article via Infotrieve]
• Nerenberg, S.T.: Electrophoresis-A Practical Laboratory Manual, Philadelphia: F.A. Davis Co., 1966, pp 218-231.
• Meyer, T.S., and Lamberts, B.L.: Use of Coomassie Brilliant Blue R250 for the Electrophoresis of Microgram Quantities of Parotid Saliva Proteins on Acrylamide Gel Strips, Biochim Biophys Acta 107: 144-145, 1965.[Medline] [Order article via Infotrieve]
• Perkin-Elmer Corp.: Analytical Methods for Atomic Absorption Spectrophotometry, revised ed, Norwalk, Conn: Perkin-Elmer Corp., 1968, p Ca8.
• Altman, P.L., and Dittmer, D.S.: Blood and Other Body Fluids, Washington, DC: Federation of American Societies for Experimental Biology, 1961, p 399.
• Seidell, A.: Solubilities of Inorganic and Metallic Organic Compounds, Vol 1, 3rd ed, New York: D. Van Nostrand Co., Inc., 1953, 292.
• Papanayotou, P.H.; Dozi-Vassiliades, J.; and Kovatsis, A.: Free Amino Acids in, Human Saliva, J Dent Res 52: 418-419, 1973.[Abstract/Free Full Text]
• Moor, J.R., and Gilligan, D.R.: Paper Partition Chromatography of Free Amino Acids and Peptides of Normal Human Saliva, J Natl Cancer Inst 12: 691-697, 1951.[Medline] [Order article via Infotrieve]
• Dawes, C.: Disodium Ethylendiaminetetraacetate As an Aid for the Reconstitution of Lypophilized Human Salivary Proteins Before Paper Electrophoresis, Arch Oral Biol 8: 653-656, 1963.[CrossRef]
• Fridhandler, L.; Berk, J.E.; and Wong, D.: Affinity Characteristics of Amylase-Binding Substance (s) Prepared from Macroamylase Complexes, Clin Chem 20: 22-25, 1974.[Medline] [Order article via Infotrieve]
• Bellavia, S.L.; Galmarini, M.; and Yan-Torno, C.: Localization of Immunoglobulin A in Electrophoretic Patterns of Proteins from Human Parotid Saliva, J Dent Res 51: 1555-1560, 1972.[Abstract/Free Full Text]
• Robinovitch, M.R., and Sreebny, L.M.: Separation of Rat Parotid Isoamylases by Preparative Polyacrylamide Gel Electrophoresis, Arch Oral Biol 15: 1381-1384, 1970.[Medline] [Order article via Infotrieve]
• Abe, K.; Sugihara, R.; Fujita, S.; Tokunaga, A.; Abe, T.; and Yokota, Y.: Disc Electrophoresis of Intact Human Saliva, J Osaka Odont Soc 32: 200-209, 1969.
• Joos, R.W., and Carr, C.W.: The Binding of Calcium to Phospholipid-Protein Complexes, Proc Soc Exp Biol Med 132: 865-870, 1969.[CrossRef][Medline] [Order article via Infotrieve]
• Eilberg, R.G.; Gould, D.; and Sobel, A.E.: Nucleating Substances in Human Saliva, Nature ( Lond) 207: 481-483, 1965.[CrossRef][Medline] [Order article via Infotrieve]
• Bettelheim, F.A.: On the Aggregation of a Calcium Precipitable Glycoprotein from Human Submaxillary Saliva, Biochim Biophys Acta 236: 702-705, 1971.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 55, No. 3, 470-475 (1976)
DOI: 10.1177/00220345760550032801


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