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Electron Microscope Comparison of Freeze-Substitution and Conventional Chemical Fixation of Undecalificied Human Dentin

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

J. David Brilliant

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

D.W. Foreman

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

Undecalcified human dentin was prepared for electron microscopy by conventional chemical fixation with alcoholic dehydration and by freeze-substitution with cryodehydration. Comparisons are reported concerning effects of different preparation methods. Morphology remained unaltered in the pre-dentin and mineralized matrices. The technique comparison permitted inferences regarding hydration states and relative amounts of protein in different areas of the odontoblast cell.

REFERENCES

• Bullivant, S.: Present Status of Freezing Techniques, in Parsons, D.F. (ed): Some Biological Techniques in Electron Microscopy, New York: Academic Press, Inc., 1970, pp 101-146.
• Kay, D.H. (ed): Techniques for Electron Microscopy, 2nd ed, Philadelphia: F.A. Davis, 1965, pp 206-207.
• Bennett, H.S., and Luft, J.H.: s-Collidine as a Basis for Buffering Fixatives, J Biophys Biochem Cytol 6: 113-114, 1959.[Medline] [Order article via Infotrieve]
• Reynolds, E.S.: The Use of Lead Citrate at High pH as an Electron Opaque Stain in Electron Microscopy, J Cell Biol 17: 208-212, 1963.[Free Full Text]
• Humason, G.L.: Animal Tissue Techniques, 2nd ed, San Francisco: W. H. Freeman & Co., 1967, p 493.
• Frank, R.M.: Ultrastructural Relationship Between the Odontoblast, Its Process, and the Nerve Fiber, in Symons, N.B.B. (ed): Dentin and Pulp: Their Structure and Reactions, London: E. & S. Livingstone, Ltd., 1968, pp 115-145.
• Frank, R.M., and Sognnaes, R.F.: Teeth, in Greep, R.O., and Weiss, L. (eds): Histology, 3rd ed, New York: McGraw-Hill, 1973, pp 511-538.
• Johansen, E., and Parks, H.F.: Electron-Microscopic Observations on Sound Human Dentin, Arch Oral Biol 7: 185-193, 1962.[Medline] [Order article via Infotrieve]
• Nalbandian, J., and Frank, R.M.: Microscopic electronique de l'odontoblaste humain et de son prolongement au cours de la dentinogenese, C R Soc Biol, (Paris) 156: 1498-1499, 1962.
• Noble, H.W.; Carmichael, A.F.; and Rankine, D.M.: Electron Microscopy of Human Developing Dentine, Arch Oral Biol 7: 395-399, 1962.[Medline] [Order article via Infotrieve]
• Nylen, M.U., and Scott, D.B.: An Electron Microscopic Study of Odontogenesis, J Indiana State Dent Assoc 39: 406-421, 1960.
• Roane, J.B., Foreman, D.W., Melfi, R.C.; and Marshall, F.J.: An Ultrastructural Study of Dentinal Innervation in the Adult Human Tooth, Oral Surg 25: 94-104, 1973.
• Pease, D.D.: The Preservation of Tissue Fine Structure During Rapid Freezing, J Ultrastruct Res 21:98-124, 1967.[CrossRef][Medline] [Order article via Infotrieve]
• Frank, R.M.: Attachment Sites Between the Odontoblast Process and the Intradentinal Nerve Fibers, Arch Oral Biol 13: 833-834, 1968.[Medline] [Order article via Infotrieve]
• Pease, D.C.: Eutectic Ethylene Glycol and Pure Propylene Glycol as Substituting Media for the Dehydration of Frozen Tissues, J Ultrastruct Res 21:75-97, 1967.[Medline] [Order article via Infotrieve]
• Dallam, R.D.: Determination of Protein and Lipid Lost During Osmic Acid Fixation of Tissues and Cellular Particulates, J Histochem Cytochem 5: 178-181, 1957.[Medline] [Order article via Infotrieve]
• Luft, J.H., and Wood, R.L.: The Extraction of Tissue Protein During and After Fixation with Osmium Tetroxide in Various Buffer Systems, J Cell Biol 19 (suppl): 46A, 1963.
• Hayat, M.A.: Principles and Techniques of Electron Microscopy, Vol 1, New York: Van Nostrand Reinhold Co., 1970, pp 35-105.
• Sabatini, D.D.; Bensch, K.; and Barrnett, R.J.: Cytochemistry and Electron Microscopy: The Preservation of Cellular Structure and Enzymatic Activity by Aldehyde Fixation, J Cell Biol 17: 19-58, 1963.[Abstract/Free Full Text]
• Meryman, H.T. (ed): Cryobiology, New York: Academic Press, Inc., 1966, p 1.
• Ling, G.N.: All or-None Adsorption by Living Cells and Model Protein-Water Systems: Discussion of the Problem of "Permease-Induction" and Determination of Secondary and Tertiary Structures of Proteins, Fed Proc 25: 958-970, 1966.[Medline] [Order article via Infotrieve]
• Weiss, L.: The Cell, in Greep, R.O., and Weiss, L. (ed): Histology, 3rd ed, New York: McGraw-Hill, 1973, pp 1-83.
• Reith, R.J.: Collagen Formation in Developing Molar Teeth of Rats, J Ultrastruct Res 21: 383-414, 1968.
• Sicher, H., and Bhaskar, S.N. (eds): Orban's Oral Histology and Embryology, 7th ed, St. Louis: C. V. Mosby, 1972, pp 99-100.

Journal of Dental Research, Vol. 55, No. 3, 400-410 (1976)
DOI: 10.1177/00220345760550031801


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hicks, M. L. Articles by Foreman, D.W. Search for Related Content PubMed PubMed Citation Articles by Hicks, M. L. Articles by Foreman, D.W. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Social Bookmarking                         What's this?