This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Strom, A. Articles by Tribble, J. L. Search for Related Content PubMed PubMed Citation Articles by Strom, A. Articles by Tribble, J. L. Social Bookmarking                         What's this?

Identification and Characterization of Species of the Family Bacteriodaceae by Polyacrylamide Gel Electrophoresis

College of Dentistry and School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68503, USA

J.K. Dyer

College of Dentistry and School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68503, USA

Connell Marsh

College of Dentistry and School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68503, USA

Jack L. Tribble

College of Dentistry and School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68503, USA

A method is described for identifying and characterizing microorganisms of the family Bacteroidaceae by their protein profiles. Protein profiles of six species and several strains of this family were compared using gradient polyacrylamide gel electrophoresis in the slab form. The protein profiles were sufficiently distinct and reproducible to allow identification of species, subspecies, and even minor strain differences. This technique is suggested as an adjunct to the present methods of characterization and identification of these microorganisms.

REFERENCES

• Smith, L., and Holdeman, L.V.: The Pathogenic Anaerobic Bacteria, Springfield, Ill: Charles C Thomas, 1968.
• Sutter, V.L.; Attebery, H.R.; Rosenblatt, J.E.; Bricknfll, K.S.,; and Finegold, S.M.: Anaerobic Bacteriology Manual, Los Angeles: UCLA Extension Division, 1972.
• Theodore, R.S.; Tully, J.G.; and Cole, R.M.: Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin, Appl Microbiol 21:272-277, 1972.
• Dyer, J.K., and Anderson, A.W.: Disc Gel Electrophoresis: An Aid in the Identification of the Various Types of Clostridium botulinum, J Dent Res 50: 431, 1971.
• Affronti, L.R.; Wright, G.L., JR.; and Reich, M.: Characterization and Comparison of Mycobacterial Antigens by Discontinuous Pore Gradient Gel Electrophoresis, Infect Immun 5: 474-481, 1972.[Abstract/Free Full Text]
• Simonson L.G., and Shklair, I.L.: Gel Electrophoresis of Intra-Cellular Components of Some Cariogenic Streptococci, J Dent Res 51: 488-491, 1972.[Abstract/Free Full Text]
• El-Sharkay, T.A., and Huisingh, D.: Differentiation Among Xanthomonas Species by Polyacrylamide Gel Electrophoresis of Soluble Proteins, J Gen Microbiol 68: 155-165, 1972.
• Holdeman, L.V., and Moore, W.E.C.: Anaerobic Laboratory Manual, Blacksburg, Va: Virginia Polytechnic Institute and State University, 1972.
• Wright, G.L., JR.; Farrell, K.B.; and Rob-Erts, D.B.: Gradient Polyacrylamide Gel Electrophoresis of Human Serum Proteins: Improved Discontinuous Gel Electrophoretic Technique and Identification of Serum Components, Clin Chim Acta 32: 285-296, 1971.[CrossRef][Medline] [Order article via Infotrieve]
• Prevot, A.R.: Manual for the Classification and Determination of Anaerobic Bacteria, 1st ed (American) FREDETTE, V. (trans), Philadelphia: Lea & Febiger, 1966.
• Finegold, S.M.; Harada, N.E.; and Miller, L.G.: Antibiotic Susceptibility Patterns as Aids in Classification and Characterization of Gram-Negative Anaerobic Bacilli, J Bacteriol 94: 1443-1450, 1967.[Abstract/Free Full Text]
• Ornstein, L.: Disc Electrophoresis: I. Background and Theory, Ann NY Acad Sci 121: 321-349, 1967.

Journal of Dental Research, Vol. 55, No. 2, 252-256 (1976)
DOI: 10.1177/00220345760550021501


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Strom, A. Articles by Tribble, J. L. Search for Related Content PubMed PubMed Citation Articles by Strom, A. Articles by Tribble, J. L. Social Bookmarking                         What's this?