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Characteristics of an In Vitro Dental Pellicle
Doris J. Stiefel
Center for Research in Oral Biology, University of Washington, Seattle, Washington 98195, USA
Dental pellicle was formed in vitro after two-hour incubation of extracted human molars in parotid saliva. Control teeth were incubated in distilled water. Electropherograms of proteins subsequently eluted from the teeth indicated selective salivary adsorption and desorption. In addition, extra-salivary pellicle constituents, comprising albumin and alkaline phosphatase, were identified.
REFERENCES
- Dawes, C.; Jenkins, G.N.; and Tonge, C.H.: Nomenclature of the Integuments of the Enamel Surface of Teeth, Br Dent J 153: 65-68, 1963.
- Meckel, A.H.: The Formation and Properties of Organic Films on Teeth, Arch Oral Biol 10: 585-597, 1965.[CrossRef][Medline]
[Order article via Infotrieve]
- Armstrong, W.G.: The Composition of Organic Films Formed on Human Teeth, Caries Res 1: 89-103, 1967.[Medline]
[Order article via Infotrieve]
- Ericson, T.: Salivary Glycoproteins-Composition and Adsorption to Hydroxylapatite in Relation to the Formation of Dental Pellicles and Calculus, Acta Odontol Scand 26: 3-21, 1968.[CrossRef][Medline]
[Order article via Infotrieve]
- Belcourt, A.; Frank, R.M.; and Houver, G.: Amino Acid Analysis of the Acquired Exogenous Pellicle and the Proteins of Human Superficial Enamel, J Biol Buccale 2: 161-179, 1974.[Medline]
[Order article via Infotrieve]
- McGaughey, C., and Stowell, E.C.: Adsorption of Salivary Proteins by Hydroxyapatite: Effect of Phosphate Ions and Interdependence of Effects of Phosphate and Hydrogen Ions, J Dent Res 53: 121-126, 1974.[Abstract/Free Full Text]
- Armstrong, W.G.: Characterization Studies on the Specific Human Salivary Proteins Adsorbed In Vitro by Hydroxyapatite, Caries Res 5: 215-227, 1971.[Medline]
[Order article via Infotrieve]
- Hay, D.I.: Some Observations on Human Saliva Proteins and Their Role in the Formation of the Acquired Enamel Pellicle, J Dent Res 48: 806-810, 1969.[Abstract/Free Full Text]
- Hay, D.I.: The Interaction of Human Parotid Salivary Proteins with Hydroxyapatite, Arch Oral Biol 18: 1517-1529, 1973.[CrossRef][Medline]
[Order article via Infotrieve]
- Pruitt, K.M.; Caldwell, R.C.; Jamieson, A.D.; and Taylor, R.E.: The Interaction of Salivary Proteins with Tooth Surface, J Dent Res 48: 818-823, 1969.[Abstract/Free Full Text]
- Hay, D.I.: The Adsorption of Salivary Proteins by Hydroxyapatite and Enamel, Arch Oral Biol 12: 937-946, 1967.[CrossRef][Medline]
[Order article via Infotrieve]
- Mayhall, C.W.: Concerning the Composition and Source of the Acquired Enamel Pellicle of Human Teeth, Arch Oral Biol 15: 1327-1341, 1970.[CrossRef][Medline]
[Order article via Infotrieve]
- Sonju, T., and Rolla, G.: Chemical Analysis of the Acquired Pellicle Formed in Two Hours on Cleaned Human Teeth In Vivo, Caries Res 7: 30-38, 1973.[Medline]
[Order article via Infotrieve]
- Sonju, T.: CHRISTENSEN, T.B.; KORNSTAD, L.; and RoLLA, G.: Electron Microscopy , Carbohydrate Analyses and Biological Activities of the Proteins Adsorbed in Two Hours on Tooth Surfaces In Vivo, Caries Res 8: 113-122, 1974.
- Mandel, I.D.: Relation of Saliva and Plaque to Caries, J Dent Res 53: 246-266, 1974.[Free Full Text]
- Curby, W.A.: Device for Collection of Human Parotid Saliva, J Lab Clin Med 41: 493-496, 1953.[Medline]
[Order article via Infotrieve]
- Dawes, C.: The Effects of Flow Rate and Duration of Stimulation on the Concentrations of Protein and the Main Electrolytes in Human Parotid Saliva, Arch Oral Biol 14: 277-294, 1969.[CrossRef][Medline]
[Order article via Infotrieve]
- Dawes, C., and Chebib, F.S.: The Influence of Previous Stimulation and the Day of the Week on the Concentrations of Protein and the Main Electrolytes in Human Parotid Saliva, Arch Oral Biol 17: 1289-1301, 1972.[CrossRef][Medline]
[Order article via Infotrieve]
- Ferguson, D.B.; and Fort, A.: Circadian Variations in Calcium and Phosphate Secretion from Human Parotid and Submandibular Salivary Glands, Caries Res 7: 19-29, 1973.[Medline]
[Order article via Infotrieve]
- Lowry, O.H.; Roseborough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275, 1951.[Free Full Text]
- Davis, B.J.: Disc Electrophoresis: II. Method and Application to Human Serum Protein, Ann NY Acad Sci 121: 404-427, 1964.[Medline]
[Order article via Infotrieve]
- Reisfeld, R.A.; Lewis, V.J.; and Williams, D.E.: Disc Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels, Nature (Lond) 195: 281-283, 1962.[CrossRef][Medline]
[Order article via Infotrieve]
- Neidle, A., and Waelsch, H.: Histones: Species and Tissue Specificity, Science 145: 1059-1061, 1964.[Abstract/Free Full Text]
- Stiefel, D.J., and Keller, P.J.: Preparation and Some Properties of Human Pancreatic Amylase Including a Comparison with Human Parotid Amylase, Biochim Biophys Acta 302: 345-361, 1973.[Medline]
[Order article via Infotrieve]
- Linhardt, K., and Walter, K.: Phosphatases (Phosphomonoesterases), in Bercmeyer, H.U. (ed): Methods of Enzymatic Analysis, Germany: Verlag Chemie, 1963, pp 779-787.
- Laurell, C.B.: Antigen-Antibody Crossed Electrophoresis, Anal Biochem 10: 358-361, 1965.[CrossRef][Medline]
[Order article via Infotrieve]
- Keller, P.J., Wanatane, S.: Personal communication, 1974.
- Bellavia, S.L.; Galmarini, M.; and Yan-Torno, C.: Localization of Immunoglobulin A in Electrophoretic Patterns of Proteins from Human Parotid Saliva, J Dent Res 51: 1555-1560, 1972.[Abstract/Free Full Text]
- Oppenheim, F.G., and Hay, D.I.: Further Studies of Human Serum Albumin in Oral Fluid, Helv Odontol Acta 16: 22-26, 1972.[Medline]
[Order article via Infotrieve]
- Gibbons, R.J., and Van Houte, J.: On the Formation of Dental Plaques, J Periodontol 44: 347-360, 1973.[Medline]
[Order article via Infotrieve]
- Orstavik, D., and Kraus, F.W.: The Acquired Pellicle: Enzyme and Antibody Activities, Scand J Dent Res 82: 202-205, 1974.[Medline]
[Order article via Infotrieve]
- Sonju, T.; Glantz, P.O.; and Rolla, G.: Protein Adsorption to Solid Surfaces in the Mouth, J Dent Res 53 (Special Issue): Abstract No. 188, 1974.
- Ten Cate, A.R.: The Distribution of Alkaline Phosphatase in the Human Tooth Germ, Arch Oral Biol 7: 195-205, 1962.[Medline]
[Order article via Infotrieve]
- Leonard, E.P., and Provenza, D.V.: Alkaline Phosphatase Activity in Sequential Mouse Molar Tooth Development-An Electron Microscopic Study, Histochemie 34: 343-354, 1973.[Medline]
[Order article via Infotrieve]
- Weatherall, J.A.; Robinson, C.; and Halls-Worth, A.S.: Variations in the Chemical Composition of Human Enamel, J Dent Res 53: 180-192, 1974.[Free Full Text]
- Moreno, E.C., and Zahradnik, R.T.: Chemistry of Enamel Subsurface Demineralization In Vitro, J Dent Res 53: 226-243, 1974.[Free Full Text]
Journal of Dental Research, Vol. 55, No. 1,
66-73 (1976)
DOI: 10.1177/00220345760550012601

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