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Biochemical and Morphological Studies of Rat Submandibular Gland: I. Centrifugal Fractionation of Granule-Rich Fraction

Institute of Dental Research, Departments of Oral Pathology and Oral Biology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48104, USA

S.G. Chakrabarti

Institute of Dental Research, Departments of Oral Pathology and Oral Biology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48104, USA

Submandibular glands from male rats were homogenized in 0.34 M sucrose and 0.5 mM ethylenediaminetetraacetic acid in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through nuclepore filters to prepare a granule-rich fraction. Electron dense zymogen granules constituted approximately 85% of the particles in this fraction which also contained about a third of the total alkaline esterase activity in the gland.

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Journal of Dental Research, Vol. 54, No. 5, 938-947 (1975)
DOI: 10.1177/00220345750540053201


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