Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Schuster, G. S.
Right arrow Articles by Harms, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schuster, G. S.
Right arrow Articles by Harms, W. S.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Effect of Exogenous Lipid on Lipid Synthesis by Bone and Bone Cell Cultures

George S. Schuster

Departments of Oral Biology and Cell and Molecular Biology, Schools of Dentistry and Medicine, Medical College of Georgia, Augusta, Georgia 30902, USA

Thomas R. Dirksen

Departments of Oral Biology and Cell and Molecular Biology, Schools of Dentistry and Medicine, Medical College of Georgia, Augusta, Georgia 30902, USA

William S. Harms

Departments of Oral Biology and Cell and Molecular Biology, Schools of Dentistry and Medicine, Medical College of Georgia, Augusta, Georgia 30902, USA

Newborn rat calvaria and isolated calvaria cells are capable of de novo lipid synthesis when grown in the presence or absence of exogenous lipid sources. Synthesis decreases when exogenous lipids are supplied. Several cholesterol precursors were found in these tissues and the presence of dihydrocholesterol was established for the first time.

REFERENCES

  • Dirksen, T.R.; Marinetti, G.V.; and Peck, W.A.: Bone and Bone Cell Lipid Metabolism: I. The In Vitro Incorporation of 14C-glycerol and 14C-glucose into Lipids of Bone and Bone Cell Cultures, Biochim Biophys Acta 202: 67-79, 1970.[Medline] [Order article via Infotrieve]
  • Dirksen, T.R.; Peck, W.A.; and Marinetti, G.V.: Lipid Metabolism in Bone and Bone Cells: II. The In Vitro Incorporation of 32p-orthophosphate and 14C-serine into Lipids of Bone and Bone Cell Cultures, Biochim Biophys Acta 202: 80-90, 1970.[Medline] [Order article via Infotrieve]
  • Dirksen, T.R.: The In Vitro Incorporation of Acetate-14C into the Lipids of Newborn Rat Calvaria, Arch Biochem Biophys 134: 603-609, 1969.[CrossRef][Medline] [Order article via Infotrieve]
  • Bailey, J.M.: Cellular Lipid Nutrition and Lipid Transport, in Rothblat, G.H., and Kritchevsky, D. (eds): Lipid Metabolism in Tissue Culture Cells, Philadelphia: Wistar Institute Press, 1967, pp 85-109.
  • Bailey, J.M.; Howard, B.V.; Dunbar, L.M.; and Tillman, S.F.: Control of Lipid Metabolism in Cultured Cells, Lipids 7: 125-134, 1972. '[CrossRef][Medline] [Order article via Infotrieve]
  • Williams, C.D., and Avigan, J.: In Vitro Effects of Serum Proteins and Lipids on Lipid Synthesis in Human Skin Fibroblasts and Leukocytes Grown in Culture, Biochim Biophys Acta 260: 413-423, 1972.[Medline] [Order article via Infotrieve]
  • Irving, J.T.: Histochemical Changes in the Early Stages of Calcification, Clin Orthop Related Res 17: 92-102, 1960.
  • Irving, J.T.: Lipids and Calciphylaxis, Arch Oral Biol 10: 189-190, 1965.[Medline] [Order article via Infotrieve]
  • Irving, J.T., and Wuthier, R.E.: Further Observations on the Sudan Black Stain for Calcification, Arch Oral Biol 5:323-324, 1961.[CrossRef][Medline] [Order article via Infotrieve]
  • Peck, W.A.; Birge, S.J.; and Fedak, SA: Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation, Science 146: 1476-1477, 1964.[Abstract/Free Full Text]
  • Eagle, H.: Amino Acid Metabolism in Mammalian Cell Cultures, Science 130:432-437, 1959.[Free Full Text]
  • Chen, R.F.: Removal of Fatty Acids from Serum Albumin by Charcoal Treatment, J Biol Chem 242: 173-181, 1967.[Abstract/Free Full Text]
  • Geyer, R.P.: Uptake and Retention of Fatty Acids by Tissue Culture Cells, in Rothblat, G.H., and Kritchevsky, D. (eds): Lipid Metabolism in Tissue Culture Cells, Philadelphia: Wistar Institute Press, 1967, pp 33-44.
  • Dirksen, T.R., and McPherson, J.C.: Effect of Metabolic Inhibitors on Bone Lipid Synthesis from "C-acetate, Int J Biochem 4: 102-106, 1973.[Medline] [Order article via Infotrieve]
  • Folch, J.; Lees, M.; and Sloane, S.: A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J Biol Chem 226: 497-509, 1957.[Free Full Text]
  • Marinetti, G.V.: Chromatographic Separation, Identification, and Analysis of Phosphatides, J Lipid Res 3: 1-20, 1962.
  • Rouser, G.; Fleischer, S.; and Yamamoto, A.: Two Dimensional Thin Layer Chromatographic Separation of Polar Lipids and Determination of Phospholipids by Phosphorus Analysis of Spots, Lipids 4: 494, 1969.
  • Marinetti, G.V.; Erbland, J.F.; and Bros-Sard, M.: Biosynthesis of Phospholipids and Glycerides, in Dawson, R.M.C., and Rhodes, D.N. (eds): Metabolism and Physiological Significance of Lipids, New York: John Wiley & Sons, Ltd., 1964, pp 71-93.
  • Wuthier, R.E.: Two-Dimensional Chromatography on Silicic Gel-Loaded Paper for the Microanalysis of Polar Lipids, J Lipid Res 7: 544-550, 1966.[Abstract]
  • Bray, G.A.: A Simplified Efficient Liquid Scintillator for Counting Aqueous Solutions in a Liquid Scintillation Counter, Anal Biochem 1: 279-285, 1960.[CrossRef]
  • Entenman, C.: General Procedures for Separating Lipid Components of Tissue, in Colowich, S.P., and Kaplan, N.O. (eds): Methods of Enzymology, New York: Academic Press, Vol 3, 1957, pp 299-391.
  • Morris, L.J.: Separation of Higher Fatty Acid Isomers and Vinylogues by Thin Layer Chromatography, Chem Ind (London) 1238-1240, 1962.
  • DE Vries, B., and Jurriens, G.: Use of Silica Gel Impregnated with Silver Nitrate for the Separation of Lipids by Thin Layer Chromatography, Fette Seifen Anstrichm 65: 725-727, 1963.[CrossRef]
  • Avigan, J.; Dewitt, S.G.; and Steinberg, D.: Thin Layer Chromatography of Sterols and Steroids, J Lipid Res 4: 100-101, 1963.[Medline] [Order article via Infotrieve]
  • Kaufmann, H.P.; Wessels, H.; and Bondo-Padhyaya, C.: Thin Layer Chromatography of Fats: XI. The Analysis of the Lecithins and the Hydrolytic Degradation Products of Phosphatides, Fette Seifen Anstrichm 65: 543-547, 1963.[CrossRef]
  • Copius-Peereboom, J.W., and Beekes, H.W.: Analysis of Mixtures of Animal and Vegetable Fats: V. Separation of Sterol Acetate by Thin Layer Chromatography in Reverse-Phase Systems and on Silica Gel G Silver Nitrate Layers, J Chromatogr 17:99-113, 1965.[CrossRef][Medline] [Order article via Infotrieve]
  • Zak, B.; Moss, N.; Boyle, A.J.; and Zlatkis, A.: Reactions of Certain Unsaturated Steroids with the Acid Iron Reagent, Anal Chem 26: 776-777, 1954.
  • Frantz, I.D., JR., and Schroepfer, G.J., JR.: Sterol Biosynthesis, Ann Rev Biochem 36: 656-726, 1967.
  • Dempsey, M.E.: Pathways of Enzymic Synthesis and Conversion to Cholesterol of -5, 7, 24-cholestatrien-3 β-ol and Other Naturally Occurring Sterols, J Biol Chem 240: 4176-4188, 1965.[Free Full Text]
  • Beshoff, F., and Turner, J.G., JR.: Spectroscopic Data for Steroids Subjected to Zak Color Reaction, Clin Chem 4: 300-303, 1968.
  • Zlatkis, A.; Zak, B.; and Boyle, A.J.: A New Method for the Direct Determination of Serum Cholesterol, J Lab Clin Med 41: 486-492, 1953.[Medline] [Order article via Infotrieve]
  • Claude, J.R., and Beaumont, J.L.: Les transformations de la molecule de cholesterol in vitro. Leur importance au cours des analyses chromatographiques, J Chroma-tog 21:189-201, 1966.[CrossRef][Medline] [Order article via Infotrieve]
  • Van Leer, J.E., and Smith, L.L.: Chromatography of Some Cholesterol Autoxidation Products on Sephadex LH20. J Chromatogr 41: 37-42, 1969.[Medline] [Order article via Infotrieve]
  • Zakim, D., and Herman, R.H.: Regulation of Fatty Acid Synthesis, Am J Clin Nutr 22: 200-213, 1969.[Medline] [Order article via Infotrieve]
  • Bortz, W.M., and Lynen, F.: The Inhibition of Acetyl-CoA Carboxylase by Long Chain CoA Derivatives, Biochem Z 56: 658, 1963.
  • Levy, H.R.: Inhibition of Mammary Gland Actyl-CoA Carboxylase by Fatty Acids, Biochim Biophys Res Commun 13: 267, 1963.[CrossRef]
  • Jacobs, R.A.; Sly, W.S.; and Majerus, P.W.: The Regulation of Fatty Acid Biosynthesis in Human Skin Fibroblasts, J Biol Chem 248: 1268-1276, 1973.[Abstract/Free Full Text]
  • Boone, C.W.; Montel, N.; Caruso, T.D.; Kazam, E.; and Stevenson, R.E.: Quality Control Studies on Fetal Bovine Serum Used in Tissue Culture, In Vitro 7: 174-189, 1972.[CrossRef]
  • Scallen, T.J.; Dhar, A.K.; and Loughran, E.D.: Isolation and Characterization of C-4 Methyl Intermediates in Cholesterol Biosynthesis after Treatment of Rat Liver In Vitro with Cholestan-3 β, 5 a, 6 β-triol, J Biol Chem 246: 3168-3174, 1971.[Abstract/Free Full Text]
  • Scallen, T.J.; Schuster, M.W.; and Dhar, A.K.: Evidence for a Noncatalytic Carrier Protein in Cholesterol Biosynthesis, J Biol Chem 246: 224-230, 1971.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 54, No. 1, 131-139 (1975)
DOI: 10.1177/00220345750540010701


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
JDRHome page
B. Kadis, J.M. Goodson, S. Offenbacher, J.W. Bruns, and S. Seibert
Characterization of Osteoblast-like Cells from Fetal Rat Calvaria
Journal of Dental Research, November 1, 1980; 59(11): 2006 - 2013.
[Abstract] [PDF]


This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Schuster, G. S.
Right arrow Articles by Harms, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schuster, G. S.
Right arrow Articles by Harms, W. S.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?