This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kamrin, B. B. Search for Related Content PubMed PubMed Citation Articles by Kamrin, B. B. Social Bookmarking                         What's this?

Induced Collagenolytic Activity by Electrical Stimulation of Embryonic Fibroblasts in Tissue Culture

Department of Biology, New York University, Washington Square, New York 10003, USA

Continuous electrical stimulation (3.5 µ amp; 1 sine wave cycle per second) of tissue culture human embryonic fibroblasts in the presence of HCL demineralized bone significantly transformed the normal cells. After 8 to 12 days of stimulation, the cells lost the capacity to form sheets; the central nucleus moved peripherally and the cells acted as phagocytes; and multinuclear cells in association with bone showed osteoclastlike properties.

REFERENCES

• Jacobsen, N., and Goldhaber, P.: Bone Resorption Induced by Epithelial and Connective Tissue from Human Gingiva In Vivo, J Dent Res 51: 1682, 1972.[Free Full Text]
• Taylor, A.C.: Collagenolysis in Cultured Tissue: I. Digestion of Mesenteric Fibers by Enzymes from Explanted Gingival Tissue, J Dent Res 50: 1294-1299, 1971.[Abstract/Free Full Text]
• Bassett, C.A.L.: Biophysical Principles Affecting Bone Structure, in Bourne, G.H. (ed): The Biochemistry and Physiology of Bone, 2nd ed, Vol III, New York: Academic Press, 1972, pp 1-72.
• Bassett, C.A.L., and Pawluk, R.J.: Electrical Behavior of Cartilage During Loading, Science 178: 982-983, 1972.[Abstract/Free Full Text]
• Bassett, C.A.L.: Biological Significance of Piezoelectricity, Calcif Tissue Res 1: 252-272, 1968.[CrossRef][Medline] [Order article via Infotrieve]
• Becker, R.O.: Stimulation of Partial Limb Regeneration in Rats, Nature (London) 235: 109-111, 1972.[CrossRef][Medline] [Order article via Infotrieve]
• Becker, R.O., and Marino, A.A.: Piezoelectric Effect and Growth Control in Bone, Nature ( London) 228:473-474, 1970.[Medline] [Order article via Infotrieve]
• Becker, R.O., and Murray, D.G.: A Method of Producing Cellular Differentiation by Means of Small Electric Current, Trans NY Acad Sci 29: 606-615, 1967.[Medline] [Order article via Infotrieve]
• Israeli, E.; Karmi, Z.; Schur, Z.; and Barzilai, D.: Collagen Development in Tissue Culture In Vitro under Static Magnetic Fields, in Menczel, J., and Harrell, A. (eds): Calcified Tissue, New York: Academic Press, 1971, pp 133-136.
• Kamrin, B.B.; Rinaldi, R.; and Liboff, A.: Piezoelectric Properties of Collagen, Anat Rec 169: 352, 1971.
• Lavine, L.S.; Lustrin, I.; Shamos, M.H.; Rinaldi, R.; and Liboff, A.: Electric Enhancement of Bone Healing, Science 175: 1118-1121, 1972.[Abstract/Free Full Text]
• Marino, A.A., and Becker, R.O.: The Origin of Bio-Electric Effects in Mineralized Bone, J Dent Res 44: 1114, 1969.
• Norton, L.A., and Moore, R.R.: Bone Growth in Organ Culture Modified by an Electric Field, J Dent Res 51: 1492-1499, 1972.[Abstract/Free Full Text]
• Shamos, M.H., and Lavine, L.S.: Piezoelectricity as a Fundamental Property of Biological Tissues, Nature (London ) 213: 267-269, 1967.[CrossRef][Medline] [Order article via Infotrieve]
• Smith, S.H.: Induction of Partial Limb Regeneration in Rana pipiens by Galvanic Stimulation, Anat Rec 158: 89-97, 1967.[CrossRef][Medline] [Order article via Infotrieve]
• Eisenstein, R.: Pathological Calcification, in Bourne, G.H. (ed): The Biochemistry and Physiology of Bone, 2nd ed, Vol II, New York: Academic Press, 1972, pp 357-380.
• Hechter, O.: Reflections on General Membrane Structure: The Conference in Review, Ann NY Acad Sci 195: 506-519, 1972.[Medline] [Order article via Infotrieve]
• Ohki, S.: Excitability of Artificial Membranes as an Analogy to Excitable Biological Membrane, Ann NY Acad Sci 195: 457-477, 1972.[Medline] [Order article via Infotrieve]
• Friedenberg, Z.B.; Andrews, E.T.; Smolen-Ski, B.I.; Peal, B.W.; and Brighton, C.T.: Bone Reaction under Varying Amounts of Electric Current, Surg Gynecol Obstet 131: 894-899, 1970.[Medline] [Order article via Infotrieve]
• Hayflick, L., and Moorhead, P.S.: The Serial Cultivation of Human Diploid Cell Strains, Exp Cell Res 25: 585-621, 1961.[CrossRef][Medline] [Order article via Infotrieve]
• Hall, B.K.: The Formation of Adventitious Cartilage by Membrane Bone under the Influence of Mechanical Stimulation Applied In Vitro, Life Sci 6: 663-667, 1967.[CrossRef][Medline] [Order article via Infotrieve]
• Green, D.E.: Membrane Proteins: A Prospective, Ann NY Acad Sci 195: 150-172, 1972.[Medline] [Order article via Infotrieve]
• Green, H., and Goldberg, B.: Collagen Synthesis by Human Fibroblast Strains, Proc Soc Exp Biol Med 117: 258-261, 1964.[CrossRef][Medline] [Order article via Infotrieve]
• Hancox, N.M.: The Osteoclast, in Bourne, G.H. (ed): The Biochemistry and Physiology of Bone, 2nd ed, Vol I, New York: Academic Press, 1972, pp 45-67.
• Urist, M.R., and Strates, B.S.: Bone Morphogenetic Protein, Dent Res 50: 1392-1406, 1971.

Journal of Dental Research, Vol. 53, No. 6, 1475-1483 (1974)
DOI: 10.1177/00220345740530063001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kamrin, B. B. Search for Related Content PubMed PubMed Citation Articles by Kamrin, B. B. Social Bookmarking                         What's this?