|
Sign In to gain access to subscriptions and/or personal tools.
|
Localization of Immunoglobulin A in Electrophoretic Patterns of Proteins from Human Parotid Saliva
Salvador L. Bellavia
Department of Biological Chemistry, Faculty of Dentistry, Nationaal University of Cordoba, Department of Immunology and Serology, National University of Córdoba, Córdoba, Argentina
Mirian Galmarini
Department of Biological Chemistry, Faculty of Dentistry, Nationaal University of Cordoba, Department of Immunology and Serology, National University of Córdoba, Córdoba, Argentina
Carlos Yantorno
Department of Biological Chemistry, Faculty of Dentistry, Nationaal University of Cordoba, Department of Immunology and Serology, National University of Córdoba, Córdoba, Argentina
Proteins from human parotid saliva were separated by use of a standardized technique of electrophoresis on polyacrylamide gel. Several fractions were eluted from the gel and studied by immunochemical methods (immunodiffusion, hemagglutination, and immunoelectrophoresis). The presence of IgA was demonstrated in the three slowest bands of the pattern.
REFERENCES
- Bellavia, S.L.: Electrophoresis of Proteins from Human Parotid Saliva, Arch Oral Biol 16:751-758, 1971.[CrossRef][Medline]
[Order article via Infotrieve]
- Curby, W.A.: Device for Collection of Human Parotid Saliva, J Lab Clin Med 41:493-496, 1953.[Medline]
[Order article via Infotrieve]
- Davis, B.: Disc Electrophoresis. Part II. Method and Application to Human Serum Protein, Ann NY Acad Sci 121:404-427, 1964.[Medline]
[Order article via Infotrieve]
- Brusilow, S., and Kupchyk, L.: An Ultrafiltration Cell, Nature (Lond) 206:294, 1965.
- Battellino, L.J.; Ramos, F.A.; and Blanco, A.: Kinetic Properties of Rabbit Testicular Lactate Dehydrogenase Isozyme, J Biol Chem 243:5185-5192, 1968.[Abstract/Free Full Text]
- Ouchterlony, O.: Diffusion in Gel Methods for Immunological Analysis, Progr Allergy 5:1, 1958.[Medline]
[Order article via Infotrieve]
- Scheidegger, J.J.: Une Micro-Methode D' inmunoelectrophorese, Int Arch Appl Immunol Allergy 7:103, 1955.
- Boyden, S.V.: The Adsorption of Proteins on Erythrocytes Treated with Tannic Acid and Subsequent Hemagglutination by Antiproteinsera, J Exp Med 93:107, 1951.[Abstract]
- Lowry, O.H.; Rosebough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem 193:265-275, 1951.[Free Full Text]
- Tomasi, T.B.; Tan, E.M.; Solomon, A.; and Prendergast, R.A.: Characteristic of an Immune System Common to Certain External Secretions, J Exp Med 121:101-124, 1965.[Abstract/Free Full Text]
- : Tomasi, T.B., and Zigelbaun, S.: The Selective Occurrence of Gamma 1A Globulins in Certain Body Fluids, J Clin Invest 42:1452-1460, 1963.
- South, M.A.; Cooper, M.D.; Wollheim, F.A.; Hong, R.; and Good, R.A.: The IGA System, J Exp Med 123:615-627, 1966.[Abstract/Free Full Text]
- Brandtzaeg, P.; Fjellanger, I.; and Gjeruldsen, S.T.: Immunoglobulin M: Local Synthesis and Selective Secretion in Patients with Immunoglobulin A Deficiency, Science 160:789-791, 1968.[Abstract/Free Full Text]
- Tomasi, T.B., and Bienenstock, J.: Secretory Immunoglobulins, Adv lmmunol 9:1-96, 1968.
- Green, G.E.: Inherent Defense Mechanisms in Saliva, J Dent Res 45:624-629, 1966.[Free Full Text]
Journal of Dental Research, Vol. 51, No. 6,
1555-1560 (1972)
DOI: 10.1177/00220345720510060801
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
A.R. Johnson
Observations on Calcium-Induced Stability of Salivary Proteins in a Lipid-Extraction System
Journal of Dental Research,
May 1, 1976;
55(3):
470 - 475.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. J. Stiefel
Characteristics of an In Vitro Dental Pellicle
Journal of Dental Research,
January 1, 1976;
55(1):
66 - 73.
[Abstract]
[PDF]
|
|
|
|
|
